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Citation: LI Xia-Lan, WEI Guo-Dong, WANG Zhao-Jing, CAI E-Na, CHEN Pei-Qing. Purification and Physicochemical Characteristics of Polysaccharide from Leaf Mustard Isolated with Dilute Alkali[J]. Chinese Journal of Applied Chemistry, ;2006, 23(11): 1304-1306. shu

Purification and Physicochemical Characteristics of Polysaccharide from Leaf Mustard Isolated with Dilute Alkali

1.
Department of Bioengineering, Huaqiao University, Quanzhou 362011

Corresponding author: LI Xia-Lan,
Received Date: 24 December 2005
Available Online: 30 March 2006
Fund Project: 2005年国务院侨办基金(05Q0016) (05Q0016)

After extraction with hot water for several times,the leaf mustard was dried and then a polysaccharide named ALP-2 was isolated from the dried leaf mustard with dilute alkali extraction,alcohol precipitation,and DEAE-Sepharose FF column chromatography.ALP-2 was identified by means of cellulose acetate film electrophoresis and HPLC to be a homogeneous polysaccharide,a non-starch polysaccharide without any protein and nucleic acid.Its average molecular weight was 459 750.The analysis by virtue of HPLC after its complete hydrolysis by acid showed that ALP-2 mainly consisted of rhamnose, arabinose,glucose,galactose,and a unknown monosaccharide.
- leaf mustard,
- polysaccharide,
- isolation,
- purification,
- physicochemical characteristics
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