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Citation: HUANG Bao-Mei, YAO Cheng-Wei, CHENG Hong. Determination of Malachite Green in Fishes by Capillary Electrophoresis Combined with Conductive Detection[J]. Chinese Journal of Applied Chemistry, ;2007, 24(3): 327-330. shu

Determination of Malachite Green in Fishes by Capillary Electrophoresis Combined with Conductive Detection

1.
a Chemical Department of Mianyang Normal University, Mianyang 621000;
2.
b The 4th Institute of CARDC, Mianyang

Corresponding author: HUANG Bao-Mei,
Received Date: 23 April 2006
Available Online: 20 October 2006
Fund Project: 绵阳师范学院资助项目(07121111) (07121111)

A method for determination of Malachite green in fishes was established by high performance capillary electrophoresis using self-constructed conductivity detection system. Several parameters affecting the determination were studied, including concentration of buffer, value of pH, organic concentration, separation voltage, and sampling time. Under the following condions:uncoated fused silica capillary(45 cm×75 μm) as separation channel, HAC-NH₄AC(concentration of NH₄AC 12 mmol/L, pH=4.0) as running buffer, 20 kV as separation voltage, a linearity exists between peak height(y) and mass concentration of malachite green(c) in the range of 7.2~180 mg/L with regression equation y=2.02+102c, linearity coefficient is 0.996 2, determination limit is 1.8 mg/L and average recovery rate is from 90.0%~102.0%. The results show that the method is simple, quick, accurate and has high recovery.
- capillary electrophoresis,
- Malachite green,
- conductive detection
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