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Citation: LIU Xiao-Fei, LI Lin, SONG Lin, XIAO Mei. Effect of Low Molecular Mass Chitosan and Its Water-soluble Derivatives on DNA and mRNA[J]. Chinese Journal of Applied Chemistry, ;2007, 24(8): 893-898. shu

Effect of Low Molecular Mass Chitosan and Its Water-soluble Derivatives on DNA and mRNA

LIU Xiao-Fei ^a,, ,
LI Lin ^a ,
SONG Lin ^a ,
XIAO Mei ^b

1.
a School of Material Science & Engineering, Tianjin University, Tianjin 300072;
2.
b Tasly R & D Institute, Tianjin

Corresponding author: LIU Xiao-Fei,
Received Date: 31 August 2006
Available Online: 8 October 2007
Fund Project: 国家自然科学基金资助项目(50173019) (50173019)

The binding abilities of chitosan of low molecular mass and its water-soluble derivatives (O-CMCS and O-HECS) with of plasmids DNA pBR322 and pUC18 were studied in vitro at different mass fractions and reactive time.The results show that raw chitosan had strong effect on both plasmids and the influencing degrees were stable.The effect of the chitosan derivatives on the plasmids depended on space effect and the quantity of the amido groups.At appropriate mass fractions (larger than 1×10^-2) and relative molecular mass (2 000,5 000,8 000 and higher than 8 000),the chitosan derivatives may have strong abilities to combine with DNA.The influencing degrees of chitosan and its derivatives on the plasmids have nothing to do with time,because the combination is based on charge attraction and the process is transitory.The experiment focused on the relationship between chitosans (with molecular Mass of 3 000~5 000) and mRNA showed that raw chitosan and O-CMCS could hinder the duplication of mRNA,and O-CMCS had a greater influence.The results might be caused by stronger protonating ability and huge volume of O-CMCS.The experiment of E.coli transfection in vivo showed that chitosan and O-CMCS can not only bind DNA but also directly hinder the transfection from the DNA to E.coli.
- chitosan,
- chitosan derivative,
- binding ability,
- plasmid DNA,
- mRNA
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