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Citation: SUN Deng-Ming, HU Wen-Na, MA Wei. Preparation of Poly(L-Argenine)-Modified Electrode and Determination of Uric Acid[J]. Chinese Journal of Applied Chemistry, ;2008, 25(8): 913-917. shu

Preparation of Poly(L-Argenine)-Modified Electrode and Determination of Uric Acid

1.
Department of Chemistry, Huaibei Coal Normal College, Huaibei 235000

Corresponding author: SUN Deng-Ming,
Received Date: 26 July 2007
Available Online: 20 September 2007
Fund Project: 安徽省重点科研计划项目(07020203011) (07020203011)安徽省教育厅自然科学基金(2005KJ140) (2005KJ140)安徽省绿色化学重点实验室基金(KLSF(Ⅰ)07)资助项目 (KLSF(Ⅰ)07)

Poly(L-argenine)-modified glassy carbon electrode was prepared by cyclic voltammetry. The electrochemical behavior of uric acid at it was studied, and a novel method was proposed for the determination of uric acid. In a phosphate buffer solution of pH=7.0, a sensitive oxidation peak and a weak reduction peak were observed at E_pa=0.398 V and E_pc=0.302 V(vs Ag/AgCl) at a scan rate of 200 mV/s. The oxidation peak current was well proportional to the concentration of uric acid, and the linear ranges for the determination of uric acid were 3.0×10^-6~8.0×10^-5 mol/L and 8.0×10^-5~2.5×10^-4 mol/L by linear sweep volta-mmetry. The detection limit was 1.0×10^-6 mol/L. The method was applied to the determination of uric acid in urine with satisfactory results.
- L-argenine,
- modified electrode,
- uric acid,
- linear sweep voltammetry
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