Citation: Xiao-Yun Ren, Ying Xue, Jian Liang, Li-Sheng Ding, Xun Liao. Selective extraction of flavonoids from Ginkgo biloba leaves using human serum albumin functionalized magnetic nanoparticles[J]. Chinese Chemical Letters, ;2013, 24(12): 1099-1102. shu

Selective extraction of flavonoids from Ginkgo biloba leaves using human serum albumin functionalized magnetic nanoparticles

1.
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China

Corresponding author: Xun Liao,
Received Date: 31 May 2013
Available Online: 21 June 2013
Fund Project:

In a novel procedure, human serum albumin functionalized magnetic nanoparticles (HSA-MNPs) were used to selectively extract nine flavonoids in the extract of Ginkgo biloba leaves. The chemical structures of those flavonoids were characterized with high performance liquid chromatography coupled with mass spectrometry (HPLC-MS). The selective extraction with HSA-MNPs coupled with structural elucidation with HPLC-MS shows powerful potential for analysis of bioactive components in traditional Chinese medicines.
- Ginkgo biloba,
- Flavonoids,
- HSA-MNPs,
- High performance liquid chromatography,
- Mass spectrometry
1. [1]
  [1] L.Z. Lin, P. Chen, M. Ozcan, J.M. Harnly, Chromatographic profiles and identification of new phenolic components of Ginkgo biloba leaves and selected products, J. Agric. Food Chem. 56 (2008) 6671-6679.
2. [2]
  [2] K.L. Chiu, Y.C. Cheng, J.H. Chen, C.M.J. Chang, P.W. Yang, Supercritical fluids extraction of Ginkgo ginkgolides and flavonoids, J. Supercrit. Fluids 24 (2002) 77-87.
3. [3]
  [3] C. Gaudineau, R. Beckerman, S. Welbourn, K. Auclair, Inhibition of human P450 enzymes by multiple constituents of the Ginkgo biloba extract, Biochem. Biophys. Res. Commun. 318 (2004) 1072-1078.
4. [4]
  [4] T.L.Wadsworth, T.L. McDonald, D.R. Koop, Effects of Ginkgo biloba extract (EGb 761) and quercetin on lipopolysaccharide induced signaling pathways involved in the release of tumor necrosis factor alpha, Biochem. Pharmacol. 62 (2001) 963-974.
5. [5]
  [5] K. Yamasaki, T. Maruyama, U. KraghHansen, M. Otagiri, Characterization of site I on human serum albumin: concept about the structure of a drug binding site, Biophys. Acta 1295 (1996) 147-157.
6. [6]
  [6] L.S. Qing, Y. Xue, W.L. Deng, et al., Ligand fishing with functionalized magnetic nanoparticles coupled with mass spectrometry for herbal medicine analysis, Anal. Bioanal. Chem. 399 (2011) 1223-1231.
7. [7]
  [7] L.S. Qing, Y. Xue, Y. Zheng, et al., Ligand fishing from Dioscorea nipponica extract using human serum albumin functionalized magnetic nanoparticles, J. Chromatogr. A 1217 (2010) 4663-4668.
8. [8]
  [8] X. Wen, J. Yang, B. He, Z. Gu, Preparation of monodisperse magnetite nanoparticles under mild conditions, Curr. Appl. Phys. 8 (2008) 535-541.
9. [9]
  [9] C. Vogt, M.S. Toprak, M. Muhammed, et al., High quality and tuneable silica shell magnetic core nanoparticles, J. Nanopart. Res. 12 (2010) 1137-1147.
10. [10]
  [10] B. Liu, W.X. Xie, D.P. Wang, et al., Preparation and characterization of magnetic luminescent nanocomposite particles, Mater. Lett. 62 (2008) 3014-3017.
11. [11]
  [11] Y.B. Ji, Q.S. Xu, Y.Z. Hu, Y.V. Heyden, Development, optimization and validation of a fingerprint of Ginkgo biloba extracts by high-performance liquid chromatography, J. Chromatogr. A 1066 (2005) 97-104.
12. [12]
  [12] L. Zhang, Z.X. Xiang, Determination of the flavonoids from Ginkgo biloba extract by high performance liquid chromatography, Chin. Chem. Lett. (2002) 968-970.
13. [13]
  [13] J.J. Song, G.Z. Fang, Y. Zhang, Q.L. Deng, S. Wang, Fingerprint analysis of Ginkgo biloba leaves and related health foods by high performance liquid chromatography/ electrospray ionization mass spectrometry, J. AOAC Int. 93 (2010) 1798-1805.
14. [14]
  [14] X. Yao, G. Zhou, Y. Tang, et al., UPLC-PDA-TOF/MS coupled with multivariate statistical analysis to rapidly analyze and evaluate Ginkgo biloba leaves from different origin, Drug Test Analysis (2013).
15. [15]
  [15] M. Dubber, V. Sewram, N. Mshicileli, G.S. Shephard, I. Kanfer, The simultaneous determination of selected flavonol glycosides and aglycones in Ginkgo biloba oral dosage forms by high performance liquid chromatography-electrospray ionisation mass spectrometry, J. Pharmaceut. Biomed. 37 (2005) 723-731.
16. [16]
  [16] X.P. Ding, J. Qi, Y.X. Chang, et al., Quality control of flavonoids in Ginkgo biloba leaves by high-performance liquid chromatography with diode array detection and on line radical scavenging activity detection, J. Chromatogr. A 1216 (2009) 2204-2210.
17. [17]
  [17] A. Hasler, O. Sticher, B. Meier, Identification and determination of the flavonoids from Ginkgo biloba by high performance liquid chromatography, J. Chromatogr. 605 (1992) 41-48.
18. [18]
  [18] S. Ding, E. Dudley, S. Plummer, et al., Fingerprint profile of Ginkgo biloba nutritional supplements by LC/ESI-MS/MS, Phytochemistry 69 (2008) 1555-1564.
19. [19]
  [19] L.S. Qing, X.Q. Shan, X.M. Xu, et al., Rapid probe and isolation of bioactive compounds from Dioscorea panthaica using human serum albumin functionalized magnetic nano-particles (HSA-MNPs) based ligand fishing coupled with electrospray ionization mass spectrometry, Rapid Commun. Mass Spectrom. 24 (2010) 3335-3339.
20. [20]
  [20] Z. Jurasekova, G. Marconi, S. Sanchez-Cortes, A. Torreggiani, Spectroscopic and molecular modeling studies on the binding of the flavonoid luteolin and human serum albumin, Biopolymers 91 (2009) 917-927.
21. [21]
  [21] J.B. Xiao, T.T. Chen, H. Cao, L.S. Chen, F. Yang, Molecular property affinity relationship of flavanoids and flavonoids for HSA in vitro, Mol. Nutr. Food Res. 55 (2011) 310-317.
1. [1]
  Tian Feng , Yun-Ling Gao , Di Hu , Ke-Yu Yuan , Shu-Yi Gu , Yao-Hua Gu , Si-Yu Yu , Jun Xiong , Yu-Qi Feng , Jie Wang , Bi-Feng Yuan . Chronic sleep deprivation induces alterations in DNA and RNA modifications by liquid chromatography-mass spectrometry analysis. Chinese Chemical Letters, 2024, 35(8): 109259-. doi: 10.1016/j.cclet.2023.109259
2. [2]
  Cheng Guo , Xiaoxiao Zhang , Xiujuan Hong , Yiqiu Hu , Lingna Mao , Kezhi Jiang . Graphene as adsorbent for highly efficient extraction of modified nucleosides in urine prior to liquid chromatography-tandem mass spectrometry analysis. Chinese Chemical Letters, 2024, 35(4): 108867-. doi: 10.1016/j.cclet.2023.108867
3. [3]
  Junmeng Luo , Qiongqiong Wan , Suming Chen . Chemistry-driven mass spectrometry for structural lipidomics at the C=C bond isomer level. Chinese Chemical Letters, 2025, 36(1): 109836-. doi: 10.1016/j.cclet.2024.109836
4. [4]
  Yang Feng , Yang-Qing Tian , Yong-Qiang Zhao , Sheng-Jun Chen , Bi-Feng Yuan . Dynamic deformylation of 5-formylcytosine and decarboxylation of 5-carboxylcytosine during differentiation of mouse embryonic stem cells into mouse neurons. Chinese Chemical Letters, 2024, 35(11): 109656-. doi: 10.1016/j.cclet.2024.109656
5. [5]
  Qiongqiong Wan , Yanan Xiao , Guifang Feng , Xin Dong , Wenjing Nie , Ming Gao , Qingtao Meng , Suming Chen . Visible-light-activated aziridination reaction enables simultaneous resolving of C=C bond location and the sn-position isomers in lipids. Chinese Chemical Letters, 2024, 35(4): 108775-. doi: 10.1016/j.cclet.2023.108775
6. [6]
  Yao-Hua Gu , Yu Chen , Qing Li , Neng-Bin Xie , Xue Xing , Jun Xiong , Min Hu , Tian-Zhou Li , Ke-Yu Yuan , Yu Liu , Tang Tang , Fan He , Bi-Feng Yuan . Metabolome profiling by widely-targeted metabolomics and biomarker panel selection using machine-learning for patients in different stages of chronic kidney disease. Chinese Chemical Letters, 2024, 35(11): 109627-. doi: 10.1016/j.cclet.2024.109627
7. [7]
  Feng-Qing Huang , Yu Wang , Ji-Wen Wang , Dai Yang , Shi-Lei Wang , Yuan-Ming Fan , Raphael N. Alolga , Lian-Wen Qi . Chemical isotope labeling-assisted liquid chromatography-mass spectrometry enables sensitive and accurate determination of dipeptides and tripeptides in complex biological samples. Chinese Chemical Letters, 2024, 35(11): 109670-. doi: 10.1016/j.cclet.2024.109670
8. [8]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
9. [9]
  Wei Shao , Wanqun Zhang , Pingping Zhu , Wanqun Hu , Qiang Zhou , Weiwei Li , Kaiping Yang , Xisheng Wang . Design and Practice of Ideological and Political Cases in the Course of Instrument Analysis Experiment: Taking the GC-MS Experiment as an Example. University Chemistry, 2024, 39(2): 147-154. doi: 10.3866/PKU.DXHX202309048
10. [10]
  Lu Huang , Jiang Wang , Hong Jiang , Lanfang Chen , Huanwen Chen . On-line determination of selenium compounds in tea infusion by extractive electrospray ionization mass spectrometry combined with a heating reaction device. Chinese Chemical Letters, 2025, 36(1): 109896-. doi: 10.1016/j.cclet.2024.109896
11. [11]
  Yanhua Chen , Xian Ding , Jun Zhou , Zhaoying Wang , Yunhai Bo , Ying Hu , Qingce Zang , Jing Xu , Ruiping Zhang , Jiuming He , Fen Yang , Zeper Abliz . Plasma metabolomics combined with mass spectrometry imaging reveals crosstalk between tumor and plasma in gastric cancer genesis and metastasis. Chinese Chemical Letters, 2025, 36(1): 110351-. doi: 10.1016/j.cclet.2024.110351
12. [12]
  Haiyan Lu , Jiayue Ye , Yiping Wei , Hua Zhang , Konstantin Chingin , Vladimir Frankevich , Huanwen Chen . Tracing molecular margins of lung cancer by internal extractive electrospray ionization mass spectrometry. Chinese Chemical Letters, 2025, 36(2): 110077-. doi: 10.1016/j.cclet.2024.110077
13. [13]
  Xu Zhang , Jiang Li , Kai-Zhou Lu , Ya-Nan Yang , Jian-Shuang Jiang , Xiang Yuan , Zi-Ming Feng , Fei Ye , Pei-Cheng Zhang . Neosophoflavonoids A–C, A class of highly oxidized hybrid flavonoids from Sophora flavescens with antidiabetic effects. Chinese Chemical Letters, 2024, 35(10): 109456-. doi: 10.1016/j.cclet.2023.109456
14. [14]
  Haoyang Wang , Ronghao Zhang , Yanlun Ren , Li Zhang . A convenient method for measuring gas-liquid volumetric mass transfer coefficient in micro reactors. Chinese Chemical Letters, 2024, 35(4): 108833-. doi: 10.1016/j.cclet.2023.108833
15. [15]
  Kexin Yuan , Yulei Liu , Haoran Feng , Yi Liu , Jun Cheng , Beiyang Luo , Qinglian Wu , Xinyu Zhang , Ying Wang , Xian Bao , Wanqian Guo , Jun Ma . Unlocking the potential of thin-film composite reverse osmosis membrane performance: Insights from mass transfer modeling. Chinese Chemical Letters, 2024, 35(5): 109022-. doi: 10.1016/j.cclet.2023.109022
16. [16]
  Tong Zhang , Xiaojing Liang , Licheng Wang , Shuai Wang , Xiaoxiao Liu , Yong Guo . An ionic liquid assisted hydrogel functionalized silica stationary phase for mixed-mode liquid chromatography. Chinese Chemical Letters, 2025, 36(1): 109889-. doi: 10.1016/j.cclet.2024.109889
17. [17]
  Wangyan Hu , Ke Li , Xiangnan Dou , Ning Li , Xiayan Wang . Nano-sized stationary phase packings retained by single-particle frit for microchip liquid chromatography. Chinese Chemical Letters, 2024, 35(4): 108806-. doi: 10.1016/j.cclet.2023.108806
18. [18]
  Jianwen Zhao , Shuai Wang , Shanshan Zhao , Liwei Chen , Fangang Meng , Xuelin Tian . A non-fluorinated liquid-like membrane with excellent anti-scaling performance for membrane distillation. Chinese Chemical Letters, 2025, 36(1): 109883-. doi: 10.1016/j.cclet.2024.109883
19. [19]
  Kunsong Hu , Yulong Zhang , Jiayi Zhu , Jinhua Mai , Gang Liu , Manoj Krishna Sugumar , Xinhua Liu , Feng Zhan , Rui Tan . Nano-engineered catalysts for high-performance oxygen reduction reaction. Chinese Chemical Letters, 2024, 35(10): 109423-. doi: 10.1016/j.cclet.2023.109423
20. [20]
  Yongkang Yue , Zhou Xu , Kaiqing Ma , Fangjun Huo , Xuemei Qin , Kuanshou Zhang , Caixia Yin . HSA shrinkage optimizes the photostability of embedded dyes fundamentally to amplify their efficiency as photothermal materials. Chinese Chemical Letters, 2024, 35(8): 109223-. doi: 10.1016/j.cclet.2023.109223

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(625)
HTML views(31)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142