Citation: Cai-Sheng Wu, Ying Jin, Jin-Lan Zhang, Yan Ren, Zhi-Xin Jia. Simultaneous determination of seven prohibited substances in cosmetic products by liquid chromatography-tandem mass spectrometry[J]. Chinese Chemical Letters, ;2013, 24(6): 509-511. shu

Simultaneous determination of seven prohibited substances in cosmetic products by liquid chromatography-tandem mass spectrometry

1.
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China

Corresponding author: Jin-Lan Zhang,
Received Date: 27 January 2013
Available Online: 8 March 2013

In this paper, we developed and validated a simple, sensitive, and selective high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method to identify and measure the following prohibited substances that may be found in cosmetic products: minoxidil, hydrocortisone, spironolactone, estrone, canrenone, triamcinolone acetonide and progesterone. Chromatographic separation was performed on a Waters Symmetry C18 (100 m×2.1 mm, 3.5 μm particle size) with a gradient elution system composed of 0.2% (v/v) formic acid aqueous solution and methanol containing 0.2% (v/v) formic acid at a flow rate of 0.3 mL/min. The substances were detected using a triple quadrupole mass spectrometer in the multiple reaction monitoring mode with an electrospray ionization source. All of the calibration curves showed good linearity (r > 0.999) within the tested concentration ranges. The limit of detection was <25 pg. The relative standard deviations for intraday precision for each of the prohibited substances were <3.5% at two concentration levels (2 μg/g, 10 μg/g). The relative recovery rate for each of the prohibited substances ranged from 91.8% to 111% at three concentration levels (0.1 μg/g, 2 μg/g, 10 μg/g), including the limit of quantification. In conclusion, we have developed and validated a method that can identify seven prohibited substances in cosmetic products.
- High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS),
- Cosmetic products,
- Prohibited substances,
- Minoxidil,
- Steroids
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沈阳化工大学材料科学与工程学院沈阳 110142