Citation: Lei Nie, Guo-Bin Xu, Xiao-Yan Wang, Yun Liu, Peng-Yuan Yang. Coupling microchip electrophoresis with MALDI-TOF-MS based on a freezing technique[J]. Chinese Chemical Letters, ;2013, 24(6): 491-493. shu

Coupling microchip electrophoresis with MALDI-TOF-MS based on a freezing technique

Lei Nie ^a ,
Guo-Bin Xu ^a,b ,
Xiao-Yan Wang ^a ,
Yun Liu ^a ,
Peng-Yuan Yang ^a,b,,

1.
a Department of Chemistry, Fudan University, Shanghai 200433, China;
2.
b Institutes of Biomedical Sciences of Shanghai Medical College, Fudan University, Shanghai 200433, China

Corresponding author: Peng-Yuan Yang,
Received Date: 29 January 2013
Available Online: 31 March 2013

A freezing technique protocol was proposed for coupling microchip electrophoresis withmatrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The microfluidic flow was frozen immediately after electrophoresis on microfluidic chip and the separated analyte molecules were kept in their zone pattern in the electrophoresis. Then, the frozen-chip was lyophilized and sent into TOF-MS instrument as a MALDI target, and the analyte molecules in the microfluidic channels were subjected to analysis by mass spectrometry. This approach could eliminate sample cross-contamination, providing a new interface for microchip electrophoresis and MALDI-MS.
- Microchip electrophoresis,
- MALDI-TOF-MS,
- Peptide analysis,
- Freezing technique
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