Citation: Yu Wang, Dian-Ming Zhou, Zhan Wu, Li-Juan Tang, Jian-Hui Jiang. Terminal protection of small molecule-linked ssDNA-SWNT nanoassembly for sensitive detection of small molecule and protein interaction[J]. Chinese Chemical Letters, ;2013, 24(2): 107-110. shu

Terminal protection of small molecule-linked ssDNA-SWNT nanoassembly for sensitive detection of small molecule and protein interaction

1.
State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China

Corresponding author: Li-Juan Tang, Jian-Hui Jiang,
Received Date: 3 December 2012
Available Online: 31 December 2012
Fund Project: EU FP7-HEALTH-2010 Programme-GlycoHIT (No. 260600) (No. 2011CB911000) CSIRT Program and NSF of Hunan Province (No. 10JJ7002). (No. 260600)

The interactions between small molecules and proteins constitute a critical regulatory mechanism in many fundamental biological processes. A novel biosensing strategy has been developed for sensitive and selective detection of small molecule and protein interaction on the basis of terminal protection of small molecule-linked ssDNA-SWNT nanoassembly. The developed strategy is demonstrated using folate and its binding protein folate receptor (FR) as a model case. The results reveal the developed technique displays superb resistance to non-specific binding, very low detection limit as low as subnanomolar, and a wide dynamic range from 100 pmol/L to 500 nmol/L of FR. Thus, it may offer a simple, cost-effective, highly selective and sensitive platform for homogeneous fluorescence detection of small molecule-protein interaction and related biochemical studies.
- Small molecule-protein interaction,
- Terminal protection,
- SWNT
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沈阳化工大学材料科学与工程学院沈阳 110142