Citation: Ying Xin ZHAO, Di Hua SHANGGUAN, Rui ZHAO, Tian Sheng SHU, Guo Quan LIU. High Performance Affinity Chromatography of Antithrombin Ⅲ Based on Monodisperse Poly (glycidyl methacrylate) Beads[J]. Chinese Chemical Letters, ;2001, 12(5): 443-446. shu

High Performance Affinity Chromatography of Antithrombin Ⅲ Based on Monodisperse Poly (glycidyl methacrylate) Beads

Center for Molecular Science, Institute of Chemistry Chinese Academy of Science, Beijing 100080

Received Date: 16 October 2000

Fund Project: This work was supported by the NNSF of China, Chinese Academy of Sciences and 863 Project. We are grateful to Hualan Bioengineering Company Ltd. for providing some biological samples and financial help.

A new approach for the separation of antithrombin Ⅲ with high performance affinity chromatography (HPAC) was described. A novel monodisperse,non-porous,cross-linked poly (glycidyl methacrylate) beads (PGMA) were used as the affinity support. With the water-soluble carbodiimide,heparin was linked covalently to amino-PGMA-beads,which was prepared by amination of PGMA. The adsorbent obtained exhibits high binding activity to antithrombin Ⅲ (ATⅢ),good resolution and excellent mechanical properties and can be used under high flow rate.
- High performance affinity chromatography,
- antithrombin Ⅲ,
- heparin.
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