Citation: Bao Hai SHAO, Jian Wei YAN, Xiu Zhu XU, Xiao Yun FU. Determination of Constituents in Hair Dyes by Reversed-phase High-Performance Liquid Chromatography[J]. Chinese Chemical Letters, ;2000, 11(6): 471-472.
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A reversed-phase high-performance liquid chromatographic method for the separation and quantitation of 1,4-phenylenediamine, 4-aminophenol, 3-aminophenol, 1,3-benzendiol and 2,6-phenylenediamine in hair dyes was developed. The separation was carried out on a μ-Bondapak C18 column with methanol-water containing 0.1% triethylamine and 0.02 mol/L NH4Ac (pH=5.20) (10:90, vol./vol.) as the mobile phase. Two real samples were analyzed.
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