Citation: Qin WEI, Dan WU, Bin DU, Qing Yu OU. Studies on the Phenylfluorone-Mo(VI) Complex as Interacting Mode Spectroscopic Probe of Protein in OP Microemulsion Medium[J]. Chinese Chemical Letters, ;2004, 15(6): 667-670. shu

Studies on the Phenylfluorone-Mo(VI) Complex as Interacting Mode Spectroscopic Probe of Protein in OP Microemulsion Medium

Qin WEI ^1,2, ,
Dan WU ¹ ,
Bin DU ¹ ,
Qing Yu OU ²

1.
School of Chemistry & Chemical Engineering, Jinan University, Jinan 250022;
2.
Lanzhou Institute of Chemical Physics, Chinese Academy of Science, Lanzhou 730000

Received Date: 13 May 2003

Fund Project: Financial support from Shangdong Provincial Science Foundation (Y2000B09) is gratefully acknowledged.

The application of phenylfluorone (PF)-Mo(VI) complex as a spectroscopic probe is studied. In the presence of OP microemulsion at pH 3.04, PF-Mo(VI) complex combines protein rapidly to form a stable compound and the absorbance at 527 nm is in proportion to the concentration of protein in the range 0~16 μg mL^-1 for bovine serum albumin (BSA). OP microemuslion media is introduced into protein determination, it has increased markedly the sensitivity of the system. The molar absorption coefficient was 5.98(10⁶ L mol^-1 cm^-1 for BSA. The assay, with sensitivity, simplicity and tolerance to many foreign substances, is applied to the determination of protein in samples with satisfactory results. Moreover, the binding number of BSA with the complex, which is determined by molar ratio and slope ratio methods, is in good agreement.
- Protein,
- spectrophotometry,
- PF-Mo(VI) complex,
- microemulsion,
- enhanced sensitivity
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