Citation: Ying Hua QIU, Al Jin HUANG, Yi Liang SUN. Triethylamine or diethylenetriamine as dynamic modifier for suppressing basic protein adsorption in capillary electrophoresis[J]. Chinese Chemical Letters, ;1999, 10(3): 227-230. shu

Triethylamine or diethylenetriamine as dynamic modifier for suppressing basic protein adsorption in capillary electrophoresis

Department of Chemistry, Peking University, Beijing 100871

Received Date: 6 October 1998

Fund Project: The financial support of this work by the Doctoral Program Foundation of the National Educational Commission of China is gratefully acknowledged.

Either triethylamine or diethylenetriamine can be conveniently used as a dynamic modifier to suppress the adsorption of basic proteins in capillary zone electrophoresis.Sufficiently high column efficiencies (>2×10⁵ plates/m for cytochrome C) were obtained with either of them along with the improvement of the peak shapes and repeatability of migration time.The relationship between the adsorption suppression effect of the modifier and its structure was discussed.
- Capillary zone electrophoresis,
- basic proteins,
- dynamic modifier,
- Triethylamne,
- diethylenetriamine
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