Citation: Wen Qing WANG, Xiang DING, Jian JIANG, Guang Zhong TU, Li Bin MA, Shao Liang HONG. NMR STUDIES ON THE WEAK INTERACTION OF FOUR-MONONUCLEOTIDES WITH TRYPTAMIDE AND LEUCINAMIDE[J]. Chinese Chemical Letters, ;1993, 4(5): 431-434. shu

NMR STUDIES ON THE WEAK INTERACTION OF FOUR-MONONUCLEOTIDES WITH TRYPTAMIDE AND LEUCINAMIDE

a Department of Technical Physics, Peking University, Beijing, 100871;
b Institute of Microchemistry, Beijing, 100871

Received Date: 18 January 1993

NMR spectroscopy analysis was introduced to investigate the weak interaction of four 5'-mononucleotides with tryptamide and leucinamide. Assuming a 1:1 complex formation, the association constants were determined by nonlinear regression analysis. The association affinity of 4 mononucleotides with tryptamide decreased in the order: G > A > C > U, indicating that purine bases stack more strongly with tryptamide than pyrimidine bases. But with leucinamide the affinity decreases in the order: G > C > A=U, suggesting an amino acid-anticodonic preferential interaction. The implication of the present data to the origin of genetic code was briefly discussed.
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