Citation: He Jie, Cao Yong, Yuan Qiang. Preparation and Photocytotoxicity in vitro of Oridonin-porphyrinchitosan Microspheres[J]. Chinese Journal of Organic Chemistry, ;2017, 37(3): 759-766. doi: 10.6023/cjocx201607022 shu

Preparation and Photocytotoxicity in vitro of Oridonin-porphyrinchitosan Microspheres

He Jie ^a ,
Cao Yong ^a ,
Yuan Qiang ^b,*,,

a.
College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053
b.
College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053

Corresponding author: Yuan Qiang, yuanqiang0825@sina.com
Received Date: 14 July 2016
Revised Date: 21 October 2016

Fund Project: the Zhejiang Science and Technology Program of Traditional Chinese Medicine 2017ZB027

Figures(6)

A emulsion crosslinking method to prepare oridonin-porphyrin-chitosan microspheres with oridonin and 5-p-(6-bromohexylaminophenyl)-10, 15, 20-triphenylporphyrin-chitosan (BHP-CS) is reported, which in turn was synthesized from 5-p-(6-bromo-hexylaminophenyl)-10, 15, 20-triphenylporphyrin (BHP) and chitosan. The porphyrin grafting rate of BHP-CS reached 30.80% on average, the drug loading rate and entrapment rate of the prepared microspheres reached 12.41% and 8.72% measured by HPLC, and the accumulated release rate was 81.74% in 48 h in vitro. The thiazolyl blue tetrazolium bromide (MTT) method was used to evaluate the photocytotoxicities of these derivatives against MCF-7 cells. The results revealed that oridonin-porphyrin-chitosan microspheres showed high photocytotoxicity in concentrations of 25, 50 and 100 μmol/mL against MCF-7 cells, and the inhibit ratio of MCF-7 cells under light irradiation for 30 min were (31.55±1.70)%, (71.03±0.76)% and (82.74±0.38)%, respectively.
- oridonin,
- porphyrin,
- microspheres,
- photocytotoxicity,
- antitumor
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