Citation: Zhang Kun, Xie Xiangqian, Shi Haixia, Shen Yuemao, Wang Haoxin. ortho-Dialkyl-Substituted Aromatic Acids from Verrucosispora sp. NS0172[J]. Chinese Journal of Organic Chemistry, ;2020, 40(4): 1038-1042. doi: 10.6023/cjoc201908033 shu

ortho-Dialkyl-Substituted Aromatic Acids from Verrucosispora sp. NS0172

Zhang Kun ^a,† ,
Xie Xiangqian ^b,† ,
Shi Haixia ^a ,
Shen Yuemao ^a,b,, ,
Wang Haoxin ^a,,

a.
State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao 266237
b.
Key Laboratory of Chemical Biology(Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan 250012

Corresponding author: Shen Yuemao, yshen@sdu.edu.cn Wang Haoxin, wanghaoxin@sdu.edu.cn

^†

Received Date: 26 August 2019
Revised Date: 8 November 2019
Available Online: 11 December 2019
Fund Project: Project supported by the National Natural Science Foundation of China (Nos. 31570039, 81530091), and the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT_17R68)the National Natural Science Foundation of China 81530091the National Natural Science Foundation of China 31570039the Program for Changjiang Scholars and Innovative Research Team in University IRT_17R68

Figures(2)

Two new ortho-dialkyl-substituted aromatic acids 1 and 2 were isolated from Verrucosispora sp. NS0172. The chemical structures of 1 and 2 were determined by spectroscopic methods including 1D- and 2D-NMR and HR-ESIMS experiments. The cytotoxicity of compounds 1 and 2 was evaluated by methyl thiazolyl tetrazolium (MTT) assay, and compound 1 showed potent antiproliferative activity against human hepatocellular carcinoma SMMC-7721 (IC₅₀ 7.74 μmol·L^-1). Compounds 1 and 2 were tested for the antimicrobial and antifungal activities by filter paper disc diffusion assay, and both of them showed no evident activities at a dose of 40 μg/disc. This study is the first report of discovering of ortho-dialkyl-substi-tuted aromatic acids from Verrucosispora, which sets the foundation for future biosynthetic study of this class of natural products in Verrucosispora.
- verrucosispora,
- actinomycetes,
- natural product,
- ortho-dialkyl-substituted aromatic acids
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