Citation: Zhou Tingting, Yang Yutao, Zhou Keyan, Xu Wenzhi, Li Wei. An "Off-On" Fluorescent Probe for Biothiols and Its Application in Bioimaging[J]. Chinese Journal of Organic Chemistry, ;2019, 39(12): 3498-3504. doi: 10.6023/cjoc201906004 shu

An "Off-On" Fluorescent Probe for Biothiols and Its Application in Bioimaging

Zhou Tingting ^a,b ,
Yang Yutao ^a,b,, ,
Zhou Keyan ^a,b ,
Xu Wenzhi ^a,b ,
Li Wei ^a,b,,

a.
College of Chemistry & Environmental Science, Hebei University, Baoding 071002
b.
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Hebei University, Baoding 071002

Corresponding author: Yang Yutao, yutaoyang2016@163.com Li Wei, liweihebeilab@163.com
Received Date: 5 June 2019
Revised Date: 6 July 2019
Available Online: 1 December 2019
Fund Project: the Science Foundation for Youth of Hebei Province B2017201093the National Natural Science Foundation of China 21807021Project supported by the National Natural Science Foundation of China (No. 21807021), the Priority Strategy Project of Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education (No. ts2019004), the Science Foundation for Youth of Hebei Province (No. B2017201093) and the Hebei University Science Foundation for High-Level Personnelthe Priority Strategy Project of Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education ts2019004

Figures(7)

An "off-on" fluorescent probe CO-NBS based coumarin derivative was designed and synthesized, which can respond selectively with biothiols including cysteine (Cys), homocysteine (Hcy) and glutathione (GSH). The optical properties were studied by UV-Vis absorption spectrum and fluorescence spectrum. The results showed that probe was capable to respond rapidly, sensitively and selectively to Cys, Hcy and GSH with the detection limits of 92, 30 and 62 nmol/L, respectively. In addition, with low cytotoxicity and good biocompatibility, the probe can be successfully applied to image biothiols in living cells and in living zebrafish.
- fluorescent probe,
- coumarin derivatives,
- biothiols,
- fluorescence imaging
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