Citation: Chang Yongxina, Li Baia, Gao Yunfana, Xu Kuoxi. A Novel Schiff-Base Fluorescent Probe for Cr³⁺ and Its Bioimaging in Cells[J]. Chinese Journal of Organic Chemistry, ;2019, 39(4): 1023-1028. doi: 10.6023/cjoc201809028 shu

A Novel Schiff-Base Fluorescent Probe for Cr³⁺ and Its Bioimaging in Cells

Chang Yongxina ^a ,
Li Baia ^a ,
Gao Yunfana ^b ,
Xu Kuoxi ^a,b,,

a.
Institute of Functional Organic Molecular Engineering, Engineering Laboratory for Flame Retardant and Functional Materials of Henan Province, College of Chemistry and Chemical Engineering, Henan University, Kaifeng, 475004
b.
Henan University Mingsheng College, Kaifeng, 475004

Corresponding author: Xu Kuoxi, xukx@henu.edu.cn
Received Date: 21 September 2018
Revised Date: 29 October 2018
Available Online: 7 April 2018
Fund Project: Project supported by the National Natural Science Foundation of China (No. U1404207)the National Natural Science Foundation of China U1404207

Figures(8)

Trivalent chromium (Cr³⁺) plays an important role in the metabolismof fats, nucleic acids, carbohydrates and proteins. Moreover, Cr³⁺ is considered to be a carcinogen and is extremely harmful to humans. Therefore, Cr³⁺ fluorescent probe L was synthesized by reaction of 8-hydroxyquinolinaldehyde with thiocarbazone. The probe L showed high selectivity towards Cr³⁺ ion through the color of solution changed from colorless to yellow for naked-eye detection and significant fluorescence intensity enhanced in CH₃CN/H₂O (V:V=1:2, Tris buffer 50 mmol/L, pH=7.3) solution. The 1:1 binding stoichiometry between probe and Cr³⁺ was determined from Job's plot, fluorescence titration, ESI-MS and density functional theory (DFT) calculations. The association constant (K_a) and the detection limit for Cr³⁺ were found to be to be 1.00×10⁵ and 2.85×10^-7 mol/L, respectively. Moreover, bioimaging experiments showed that L could sense Cr³⁺ ion in living cells with a fluorescence enhancement signal.
- Cr³⁺,
- fluorescent probe,
- DFT,
- bioimaging
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A Novel Schiff-Base Fluorescent Probe for Cr3+ and Its Bioimaging in Cells

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通讯作者: 陈斌, bchen63@163.com

A Novel Schiff-Base Fluorescent Probe for Cr³⁺ and Its Bioimaging in Cells