Citation: Li Huifang, Leng Xin, Yan Lirun, Yang Bingqin, Bai Yinjuan. A Series of Red Dyes: Synthesis, Optical Properties and Application in Protein Labeling[J]. Chinese Journal of Organic Chemistry, ;2019, 39(3): 793-799. doi: 10.6023/cjoc201807036 shu

A Series of Red Dyes: Synthesis, Optical Properties and Application in Protein Labeling

Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an 710127

Corresponding author: Yang Bingqin, yangbq@nwu.edu.cn Bai Yinjuan, baiyinjuan@nwu.edu.cn
Received Date: 22 July 2018
Revised Date: 11 September 2018

Fund Project: the National Natural Science Foundation of China 21172178Project supported by the National Natural Science Foundation of China (No. 21172178)

Figures(10)

A series of red fluorescent dyes were synthesized using 3-N, N-diethylaminophenol. Their structures were characterized by ¹H NMR, ¹³C NMR, IR and HRMS. The optical properties of these dyes were detected. Bovine serum albumin was labeled by new dyes. The fluorescence signal values of these dyes and cyanine-3 (Cy3) in lectin microarrays were compared. The results show that the maximum emission wavelengths of these dyes are above 600 nm and the Stokes shifts are about 70 nm. The dye/protein (D/P) labeling efficiencies of these dyes are as high as 1.5~1.8. The fluorescence signal of new dyes is equal to Cy3 in lectin microarray. Hence, these dyes can also be used as fluorescent labels for detection of sugar chain changes in lectin microarrays.
- fluorescent dye,
- synthesis,
- spectral property,
- protein label,
- lectins microarray
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