Citation: Wang Xiaofen, Wei Chao, Li Xueyan, Zheng Xueyang, Geng Xiaowei, Zhang Pingzhu, Li Xiaoliu. Lysosome-Targeted Dual-Photon Nitroxyl Fluorescent Probe: Synthesis and Application in Living Cell Imaging[J]. Chinese Journal of Organic Chemistry, ;2019, 39(2): 469-474. doi: 10.6023/cjoc201807032 shu

Lysosome-Targeted Dual-Photon Nitroxyl Fluorescent Probe: Synthesis and Application in Living Cell Imaging

Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Key Laboratory of Chemical Biology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding 071002

Corresponding author: Wei Chao, weichao@hbu.edu.cn Li Xiaoliu, lixl@hbu.edu.cn
Received Date: 20 July 2018
Revised Date: 27 August 2018
Available Online: 17 February 2018
Fund Project: the Natural Science Foundation of Hebei Provience 2018201234the Science Technology Research and Development Guidance Program Project of Baoding City 16zg031the Colleges and Universities Science Technology Research Project of Hebei Province QN2017015Project supported by the National Natural Science Foundation of China (Nos. 21572044, 21778013), the Natural Science Foundation of Hebei Provience (Nos. B2016201031, 2018201234), the Colleges and Universities Science Technology Research Project of Hebei Province (No. QN2017015) and the Science Technology Research and Development Guidance Program Project of Baoding City (No. 16zg031)the National Natural Science Foundation of China 21778013the National Natural Science Foundation of China 21572044the Natural Science Foundation of Hebei Provience B2016201031

Figures(8)

Nitroxyl (HNO), which is the one-electron reduced and further protonated form of nitric oxide, plays important biological functions. A lysosome-targeted dual-photon HNO fluorescent probe (Lyso-HNO), which contains 4-(2-amino-ethyl)morpholine as lysosomal-targetable groups, 1, 8-naphthalimide as two-photon fluorophore and triphenylphosphine as HNO reaction site, was synthesized and characterized. The recognition behaviors of Lyso-HNO to HNO were investigated. The results showed that Lyso-HNO exhibited good selectivity and sensitivity to HNO with fast response, . and the detection limit of Lyso-HNO to HNO was estimated to be 202 nmol·L^-1. The probe can be applied to bioimaging exogenous lysosomal HNO by two-photon fluorescence confocal microscopy.
- lysosome,
- dual-photon,
- nitroxyl,
- bioimaging
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