Citation: Liu Yang, Xie Shuanshuan, Zeng Jie, Tan Min, Song Xiaolian, Zhu Jun, Wang Changhui. Preparation of Poly (lactic-co-glycolic acid)/Polyethylene Glycol Nanoparticles with Surface-Modified Aptamer S6 and Its Application on Carrier siRNA[J]. Chinese Journal of Organic Chemistry, ;2018, 38(10): 2706-2712. doi: 10.6023/cjoc201803050 shu

Preparation of Poly (lactic-co-glycolic acid)/Polyethylene Glycol Nanoparticles with Surface-Modified Aptamer S6 and Its Application on Carrier siRNA

Liu Yang ^a,† ,
Xie Shuanshuan ^a,† ,
Zeng Jie ^a ,
Tan Min ^a ,
Song Xiaolian ^a ,
Zhu Jun ^b,, ,
Wang Changhui ^a,,

a.
Department of Respiratory Medicine, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072
b.
National Engineering Research Center for Nanotechnology, Shanghai 200241

Corresponding author: Zhu Jun, yzjzhu@163.com Wang Changhui, wangch@tongji.edu.cn

^†

Received Date: 29 March 2018
Revised Date: 19 June 2018
Available Online: 22 October 2018
Fund Project: the National Natural Science Foundation of China 81472180Project supported by the National Natural Science Foundation of China (No. 81472180)

Figures(9)

Lung cancer threats human health. The poly (lactic-co-glycolic acid)/polyethylene glycol (PLGA-PEG) was synthesized by the coupling method and aptamer S6 was synthesized by the nucleic acid solid-phase phosphorimide method. 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide salt was obtained. Acid-mediated (EDC) and N-hydroxysuccinimide (NHS) mediators have resulted in a biotargeting nanocarrier material PLGA-PEG-S6-CY3. PLGA-PEG-S6-CY3/CAP1-siRNA nanoparticles were constructed by carrying the small interfering RNA (CAP1-siRNA) targeting the adenylate cyclase-associated protein 1 through the carrier material, and the sustained release of the CAP1-siRNA was achieved to achieve targeting inhibition of metastasis of non-small cell lung cancer A549 cells. The molecular structures of S6 and PLGA-PEG were characterized by nuclear magnetic resonance spectroscopy and their targeting properties were verified. The binding and release of PLGA-PEG nanoparticles and CAP1-siRNA were detected. Furthermore, the effect of the complex on inhibiting the invasiveness of tumor cells was further investigated. The results showed that the PLGA-PEG molecule prepared by the coupling method was used as the carrier, and the aptamer S6 was synthesized and used to modify PLGA-PEG. The obtained PLGA-PEG nanocomposite has the ability of targeting and carrying siRNA, and can be used in vitro. It can also down-regulate the expression of CAP1 in lung cancer A549 cells and reduce its invasiveness.
- aptamer,
- PLGA-PEG molecule,
- siRNA,
- anti-tumor invasion References
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