Citation: Wang Longwen, Ma Jimei, Cheng Xin, Li Zilong, Sun Linhao, Zeng Zhen, Jiang Hong. Determination of β-Glucosidase Activity Based on Enzyme-Triggered Click Chemistry[J]. Chinese Journal of Organic Chemistry, ;2018, 38(10): 2775-2779. doi: 10.6023/cjoc201803011 shu

Determination of β-Glucosidase Activity Based on Enzyme-Triggered Click Chemistry

Department of Chemistry, College of Science, Huazhong Agricultural University, Wuhan 430070

Corresponding author: Ma Jimei, majimei@mail.hzau.edu.cn Jiang Hong, jianghong0066@126.com
Received Date: 9 March 2018
Revised Date: 21 April 2018
Available Online: 1 October 2018
Fund Project: the National Natural Science Foundation of China 21402056Project supported by the National Natural Science Foundation of China (No. 21402056)

Figures(3)

A novel method to quantify β-glucosidase activity was developed by coupling the cleavage of 2-O-(β-glucopyra-nosyl)ascorbic acid with the reduction of Cu (Ⅱ). The in situ generated Cu (Ⅰ) catalyzed the cycloaddition between weakly fluorescent coumarin and benzyl azide to yield a highly fluorescent triazole product. The fluorescence intensity was dependently enhanced on the increase of the activity of β-glucosidase and a linear relationship was found between 1~40 U/L. This cascade allows detection of β-glucosidase with a limit of detection of 0.456 U/L.
- β-glucosidase activity,
- determination,
- 2-O-(β-glucopyranosyl)ascorbic acid,
- click chemistry
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