Citation: Zhang Min, Xiao Huifeng, Han Zhixiang, Yang Liuqing, Wu Xiangyang. A Hg²⁺-Selective Fluorescence Probe: Synthesis and Application in Aqueous Solution and Living Cell Imaging[J]. Chinese Journal of Organic Chemistry, ;2018, 38(4): 926-930. doi: 10.6023/cjoc201708061 shu

A Hg²⁺-Selective Fluorescence Probe: Synthesis and Application in Aqueous Solution and Living Cell Imaging

a.
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013
b.
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013

Corresponding author: Wu Xiangyang, wuxy@ujs.edu.cn
Received Date: 30 August 2017
Revised Date: 9 November 2017
Available Online: 1 April 2017
Fund Project: the Natural Science Foundation of Jiangsu Province BK20140536Project supported by the Natural Science Foundation of Jiangsu Province (No. BK20140536)

Figures(8)

A novel rhodamine B Schiff-base fluorescence probe 1 was synthesized and characterized by ¹H NMR, ¹³C NMR, MS and single-crystal X-ray analysis. The recognition properties of the probe 1 with Hg²⁺ ions were investigated in ethanol-water (V/V=7/3, HEPES 10 mmol/L, pH=7.0) by the UV-Vis spectrophotometry and the fluorescence spectrophotometry. The probe 1 showed remarkable "turn-on" fluorescent responses to Hg²⁺ with good selectivity over the other metal ions examined. The 1:1 binding model of probe 1 with Hg²⁺ was established by Job's plot and MS spectra. The probe can be applied to the quantitative analysis of Hg²⁺ with a linear range covering from 0 to 50 μmol/L. The fluorescence imaging experiments in MGC-803 living cells demonstrated that 1 could be used in biological systems to monitor Hg²⁺.
- rhodamine B,
- synthesis,
- fluorescent probe,
- Hg²⁺,
- bioimaging
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

A Hg2+-Selective Fluorescence Probe: Synthesis and Application in Aqueous Solution and Living Cell Imaging

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通讯作者: 陈斌, bchen63@163.com

A Hg²⁺-Selective Fluorescence Probe: Synthesis and Application in Aqueous Solution and Living Cell Imaging