Citation: Lü Mingxiu, Mai Wenpeng, Lu Kui, Duan Bingchao, Zhao Yufen. Design, Synthesis and Properties of the Antibacterial Peptides Based on Tat(49-57)[J]. Chinese Journal of Organic Chemistry, ;2018, 38(1): 148-155. doi: 10.6023/cjoc201708042 shu

Design, Synthesis and Properties of the Antibacterial Peptides Based on Tat(49-57)

Lü Mingxiu ^a,b ,
Mai Wenpeng ^b ,
Lu Kui ^b,c,, ,
Duan Bingchao ^a ,
Zhao Yufen ^a,,

a.
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001
b.
School of Material and Chemical Engineering, Henan University of Engineering, Zhengzhou 450007
c.
School of Chemical Engineering and Food Science, Zhengzhou Institute of Technology, Zhengzhou 450044

Corresponding author: Lu Kui, luckyluke@haue.edu.cn Zhao Yufen, zicb@zzu.edu.cn
Received Date: 21 August 2017
Revised Date: 23 October 2017
Available Online: 31 January 2017
Fund Project: Project supported by the National Natural Science Foundation of China (Nos. 21572046, 21172054)the National Natural Science Foundation of China 21172054the National Natural Science Foundation of China 21572046

Figures(4)

Four novel cationic antibacterial peptide analogs were modified at the N-terminus of the cell-penetrating peptide transacting activator of transcription TAT(49-57) by attaching dipeptides. Peptides were synthesized through standard Fmoc solid-phase peptide synthesis procedures, purified by reversed-phase high performance liquid chromatography (RP-HPLC), and characterized by ¹H NMR, ESI-MS and elemental analysis. Tat(YY), Tat(FF), Tat(FF) and Tat(YY), Tat(FF) demonstrated better antibacterial activities against E. coli, S. typhimurium, B. subtilis and S. aureus with low hemolysis. respectively, but had no inhibitory effect on fungus growth. The Tat(49-57) analogs inhibited the bacteria more effectively than Tat(49-57). The interactions between peptides and calf thymus DNA (ct-DNA) were investigated with multi-spectroscopic techniques. The results showed that both peptides could interact with DNA via the groove binding mode. Compared to TAT(49-57), antibacterial peptide analogs combined with DNA much closer via binding constants, which has the value to become an excellent antibacterial agent with further improved and designed.
- cell-penetrating peptide,
- solid-phase synthesis,
- antibacterial activity,
- non-covalent binding
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