Citation: Li Linlu, Lü Mingxiu, Lu Kui, Liu Guangbin, Peng Lu. Design and Synthesis of Breast Cancer Susceptibility Gene BRCA1 Analogs Peptides and the Interaction of Analogs Peptides with Breast Cancer Suppressor Gene Protein RAD51[J]. Chinese Journal of Organic Chemistry, ;2018, 38(1): 246-252. doi: 10.6023/cjoc201708041 shu

Design and Synthesis of Breast Cancer Susceptibility Gene BRCA1 Analogs Peptides and the Interaction of Analogs Peptides with Breast Cancer Suppressor Gene Protein RAD51

Li Linlu ^a ,
Lü Mingxiu ^a,b ,
Lu Kui ^b,c,, ,
Liu Guangbin ^a ,
Peng Lu ^b

a.
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001
b.
School of Material and Chemical Engineering, Henan University of Engineering, Zhengzhou 450007
c.
College of Chemical Engineering and Food Science, Zhengzhou Institute of Technology, Zhengzhou 450044

Corresponding author: Lu Kui, luckyluke@haue.edu.cn
Received Date: 21 August 2017
Revised Date: 26 October 2017
Available Online: 31 January 2017
Fund Project: the National Natural Science Foundation of China 21172054the National Natural Science Foundation of China 21572046Project supported by the National Natural Science Foundation of China (Nos. 21572046, 21172054)

Figures(3)

Peptide breast cancer susceptibility gene BRCA1 plays an active role in inhibiting female breast cancer. The interaction between BRCA1 and breast cancer suppressor gene protein RAD51 in cancer cell is also an essential part for the treatment of breast cancer. Discovery Studio simulation of the docking process of BRCA1 analogs and RAD51 was used to screen the BRCA1 analogs with different charge and acid-base properties. The R-DOCK evaluation system is used to screened out 4 higher BRCA1 like peptides from the results, and the interactions with BRCA1 analogs of RAD51 peptides (Pep158-180, Pep181-200 and Pep241-260) were studied by using fluorescence spectroscopy and CD spectroscopy. The results indicate that acid-base properties of BRCA1 analogs have great influence on their interaction. The results showed that the effect of BRCA1-3 to RAD51 (Pep158-180/Pep241-260) which is the two key peptides were obviously higher than the other parent peptide. Besides, compared with BRCA1, all BRCA1 analogs combined with RAD51 much closer. The results provide evidence to design novel breast drugs for breast cancer.
- BRCA1 peptide,
- RAD51 peptide,
- fluorescence,
- CD spectroscopy,
- DS simulation
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