Citation: Gao Yong, Wang Jun, Fu Mei, Chen Hongwei, Fang Mingzhang. A Water-Soluble Rhodamine-Based Fluorescent Probe for Fe³⁺ and Its Application in Live Cell Imaging[J]. Chinese Journal of Organic Chemistry, ;2017, 37(3): 617-623. doi: 10.6023/cjoc201610014 shu

A Water-Soluble Rhodamine-Based Fluorescent Probe for Fe³⁺ and Its Application in Live Cell Imaging

Gao Yong ^a,b,*,, ,
Wang Jun ^a ,
Fu Mei ^a ,
Chen Hongwei ^a ,
Fang Mingzhang ^a

a.
College of Chemistry and Chemical Engineering, Fujian Normal University, Fuzhou 350007
b.
Fujian Provincial Key Laboratory of Advanced Materials Oriented Chemical Engineering, Fujian Normal University, Fuzhou 350007

Corresponding author: Gao Yong, gaoyong77@126.com
Received Date: 11 October 2016
Revised Date: 15 December 2016

Fund Project: Project supported by the Natural Science Foundation of Fujian Province 2013H0019

Figures(10)

A rhodamine-based water-soluble fluorescent probe of Fe³⁺(T1) was designed and synthesized, in which a carboxy was linked to enhance its water solubility. Its structure was characterized by ¹H NMR, ¹³C NMR and HRMS-ESI. The fluorescent probe showed remarkable "turn-on" fluorescent responses to Fe³⁺ with good selectivity over other competitive cations. It has a good water solubility that the recognition properties of the probe with Fe³⁺ ions can be investigated in DMF/Tris-HCl (V:V=1:9, pH=7.4). The fluorescence imaging experiments of Fe³⁺ in MCF-7 living cells demonstrated that the probe had a good cell-membrane permeability and it could be used in biological systems.
- rhodamine,
- water solubility,
- Fe³⁺,
- fluorescent probe,
- live cell imaging
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A Water-Soluble Rhodamine-Based Fluorescent Probe for Fe3+ and Its Application in Live Cell Imaging

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A Water-Soluble Rhodamine-Based Fluorescent Probe for Fe³⁺ and Its Application in Live Cell Imaging