Citation: Wang Cong, Wang Liping, Fan Jie, Sun Kunlai, Zhu Weiming. Cytotoxic Compounds from the DeepSea Sediment-Derived Streptomyces malaysiensis OUCMDZ-2167[J]. Chinese Journal of Organic Chemistry, ;2017, 37(3): 658-666. doi: 10.6023/cjoc201609021 shu

Cytotoxic Compounds from the DeepSea Sediment-Derived Streptomyces malaysiensis OUCMDZ-2167

Wang Cong ^a ,
Wang Liping ^b ,
Fan Jie ^a ,
Sun Kunlai ^a,c ,
Zhu Weiming ^a,*,,

a.
Key Laboratory of Marine Drugs, Ministry Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003
b.
Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guizhou 550002
c.
School of Food Science and Pharmaceutics, Zhejiang Ocean University, Zhoushan 316022

Corresponding author: Zhu Weiming, weimingzhu@ouc.edu.cn
Received Date: 18 September 2016
Revised Date: 17 November 2016

Fund Project: Project supported by the National Natural Science Foundation of China 81561148012Project supported by the National Natural Science Foundation of China 41376148the National Natural Science Foundation of China-Guangdong and the National Natural Science Foundation of China-Shandong Province Joint Funds U1406402the National Natural Science Foundation of China-Guangdong and the National Natural Science Foundation of China-Shandong Province Joint Funds U1501221

Figures(2)

This paper is designed to study the bioactive natural products sourced from the deep-sea sediment-derived Strep-tomyces malaysiensis OUCMDZ-2167. By means of column chromatography on silica gel, sephadex LH-20 and semi-prepa-rative HPLC, a new furan derivative 1 and ten known compounds 2~11 were isolated and purified from the fermentation broth of S. malaysiensis OUCMDZ-2167. Their structures were respectively elucidated as methyl 4-(hydroxymethyl) furan-2-carboxylate (1), dimethyl furan-2, 4-dicarboxylate (2), geldanamycin (3), 17-O-demethylgeldanamycin (4), nigericin (5), nigericin sodium salt (6), 30-O-acetylnigericin (7), abierixin (8), elaiophylin (9), surugapyrone A (10) and germicidin A (11), by analyzing the MS, UV, IR, NMR and specific rotations. The cytotoxicities against MCF-7, A549 and K562 were evaluated by thiazolyl blue tetrazolium bromide (MTT) and the cell counting kit-8 (CCK-8) methods. The results showed that compounds 3 and 5~9 exhib-ited the cytotoxicities against A549 cell with the half inhibitory concentrations (IC₅₀) of 2.56±0.30, 0.96±0.03, 0.96±0.04, 0.81±0.05, 7.11±0.90 and 4.09±0.70 μmol·L^-1, respectively. Compounds 5~9 displayed the cytotoxicities against K562 cells with the IC₅₀ values of 1.46±0.11, 1.08±0.03, 0.48±0.10, 4.68±0.19 and 9.60±0.50 μmol·L^-1, respectively. And compounds 5, 8 and 9 showed cytotoxicities against MCF-7 cell with the IC₅₀ values of 0.29±0.15, 4.05±0.50 and 4.81±0.70 μmol·L^-1, respectively. It is the first time to report the cytotoxicities of compounds 5~8 against K562 and A549 cell lines and against MCF-7 cell line.
- deep-sea Streptomyces,
- Streptomyces malaysiensis,
- bioactive natural products,
- cytotoxicity
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