Citation: Gong Shaohua, Li Na, Tang Bo. Recent Progress in Regulating CRISPR-Cas9 System for Gene Editing[J]. Acta Chimica Sinica, ;2020, 78(7): 634-641. doi: 10.6023/A20040131 shu

Recent Progress in Regulating CRISPR-Cas9 System for Gene Editing

College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institute of Molecular and Nano Science, Shandong Normal University, Jinan 250014, China

Corresponding author: Li Na, lina@sdnu.edu.cn
Received Date: 29 April 2020
Available Online: 8 June 2020
Fund Project: the National Natural Science Foundation of China 21775094the National Key R & D Program of China 2019YFA0210100the National Natural Science Foundation of China 21535004the Youth Innovation Science and Technology Program of Higher Education Institution of Shandong Province 2019KJC022the Key Research and Development Program of Shandong Province 2018YFJH0502Project supported by the National Key R & D Program of China (No. 2019YFA0210100), the National Natural Science Foundation of China (Nos. 21535004, 91753111, 21927881, 21874086, 21775094), the Key Research and Development Program of Shandong Province (No. 2018YFJH0502), and the Youth Innovation Science and Technology Program of Higher Education Institution of Shandong Province (No. 2019KJC022)the National Natural Science Foundation of China 21927881the National Natural Science Foundation of China 21874086the National Natural Science Foundation of China 91753111

Figures(8)

Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein (Cas) system is an adaptive immune system used by many bacteria and archaea to defend the invasion of exogenous nucleic acids. CRISPR-Cas system in different species of archaea and bacteria has different components and working mechanisms. Depending on the numbers of effector proteins, CRISPR-Cas systems can be classified into two major types. CRISPR-Cas9, which is composed of Cas9 nuclease and sgRNA, belongs to class Ⅱ CRISPR-Cas system and can be used as a powerful genome editing tool. It can target and cleave the DNA sequence which contains protospacer adjacent motif (PAM, 5'-NGG-3') sequence. The DNA double-strand breaks (DSBs) can be repaired by homology-directed repair (HDR) or nonhomologous end joining (NHEJ) mechanism. Insertions or deletions (indels) can be introduced at targeted loci in the DSBs repair process. Due to its convenience, low cost and high efficiency, CRISPR-Cas9 has played an important role in promoting the development of gene editing in basic research and clinical medicine. However, off-target effect of CRISPR-Cas9 should not be neglected. The CRISPR-Cas9 is able to cleave the target DNA even when the sgRNA imperfectly matches with the target DNA, leading to the unwanted indels at nontargeted DNA loci, which limits the further application of genome editing, especially for the treatment of genetic diseases. Therefore, it is significant to reduce the off-target cleavage effect of CRISPR-Cas9. Many efforts have been devoted to realize the reduced off-target effect of CRISPR-Cas9. Among these methods, regulating the function of CRISPR-Cas9 at spatiotemporal dimension is a potential strategy to reduce the off-target effect of CRISPR-Cas9 system and improve the specificity of gene editing. In this review, we summarized the research advances in regulating the function of CRISPR-Cas9 and discussed the prospects and challenges of CRISPR-Cas9 regulation.
- CRISPR-Cas9,
- gene editing,
- specificity,
- off-target effect,
- regulation
1. [1]
  Jiang, F.; Doudna, J. A. Annu. Rev. Biophys. 2017, 46, 505. doi: 10.1146/annurev-biophys-062215-010822
2. [2]
  Garcia-Doval, C.; Jinek, M. Curr. Opin. Struct. Biol. 2017, 47, 157. doi: 10.1016/j.sbi.2017.10.015
3. [3]
  Makarova, K. S.; Haft, D. H.; Barrangou, R.; Brouns, S. J.; Charpentier, E.; Horvath, P.; Moineau, S.; Mojica, F. J.; Wolf, Y. I.; Yakunin, A. F.; van der Oost, J.; Koonin, E. V. Nat. Rev. Microbiol. 2011, 9, 467. doi: 10.1038/nrmicro2577
4. [4]
  Wang, H.; La Russa, M.; Qi, L. S. Annu. Rev. Biochem. 2016, 85, 227. doi: 10.1146/annurev-biochem-060815-014607
5. [5]
  Nunez, J. K.; Harrington, L. B.; Doudna, J. A. ACS Chem. Biol. 2016, 11, 681. doi: 10.1021/acschembio.5b01019
6. [6]
  Gasiunas, G.; Barrangou, R.; Horvath, P.; Siksnys, V. Proc. Natl. Acad. Sci. U. S. A. 2012, 109, 15539.
7. [7]
  Gutschner, T.; Haemmerle, M.; Genovese, G.; Draetta, G. F.; Chin, L. Cell Rep. 2016, 14, 1555. doi: 10.1016/j.celrep.2016.01.019
8. [8]
  Dow, L. E. Trends Mol. Med. 2015, 21, 609. doi: 10.1016/j.molmed.2015.07.006
9. [9]
  Pickar-Oliver, A.; Gersbach, C. A. Nat. Rev. Mol. Cell Biol. 2019, 20, 490. doi: 10.1038/s41580-019-0131-5
10. [10]
  LaFountaine, J. S.; Fathe, K.; Smyth, H. D. Int. J. Pharm. 2015, 494, 180. doi: 10.1016/j.ijpharm.2015.08.029
11. [11]
  Gupta, R. M.; Musunuru, K. J. Clin. Invest. 2014, 124, 4154. doi: 10.1172/JCI72992
12. [12]
  Gaj, T.; Gersbach, C. A.; Barbas, C. F. Trends Biotechnol. 2013, 31, 397. doi: 10.1016/j.tibtech.2013.04.004
13. [13]
  Doudna, J. A.; Charpentier, E. Science 2014, 346, 1258096. doi: 10.1126/science.1258096
14. [14]
  Hsu, P. D.; Lander, E. S.; Zhang, F. Cell 2014, 157, 1262. doi: 10.1016/j.cell.2014.05.010
15. [15]
  Platt, R. J.; Chen, S.; Zhou, Y.; Yim, M. J.; Swiech, L.; Kempton, H. R.; Dahlman, J. E.; Parnas, O.; Eisenhaure, T. M.; Jovanovic, M.; Graham, D. B.; Jhunjhunwala, S.; Heidenreich, M.; Xavier, R. J.; Langer, R.; Anderson, D. G.; Hacohen, N.; Regev, A.; Feng, G.; Sharp, P. A.; Zhang, F. Cell 2014, 159, 440. doi: 10.1016/j.cell.2014.09.014
16. [16]
  Carroll, K. J.; Makarewich, C. A.; McAnally, J.; Anderson, D. M.; Zentilin, L.; Liu, N.; Giacca, M.; Bassel-Duby, R.; Olson, E. N. Proc. Natl. Acad. Sci. U. S. A. 2016, 113, 338. doi: 10.1073/pnas.1523918113
17. [17]
  Wang, T.; Wei, J. J.; Sabatini, D. M.; Lander, E. S. Science 2014, 343, 80. doi: 10.1126/science.1246981
18. [18]
  Behan, F. M.; Iorio, F.; Picco, G.; Goncalves, E.; Beaver, C. M.; Migliardi, G.; Santos, R.; Rao, Y.; Sassi, F.; Pinnelli, M.; Ansari, R.; Harper, S.; Jackson, D. A.; McRae, R.; Pooley, R.; Wilkinson, P.; van der Meer, D.; Dow, D.; Buser-Doepner, C.; Bertotti, A.; Trusolino, L.; Stronach, E. A.; Saez-Rodriguez, J.; Yusa, K.; Garnett, M. J. Nature 2019, 568, 511. doi: 10.1038/s41586-019-1103-9
19. [19]
  Didovyk, A.; Borek, B.; Tsimring, L.; Hasty, J. Curr. Opin. Biotechnol. 2016, 40, 177. doi: 10.1016/j.copbio.2016.06.003
20. [20]
  Nihongaki, Y.; Furuhata, Y.; Otabe, T.; Hasegawa, S.; Yoshimoto, K.; Sato, M. Nat. Methods 2017, 14, 963. doi: 10.1038/nmeth.4430
21. [21]
  Ma, Y.; Wang, M.; Li, W.; Zhang, Z.; Zhang, X.; Wu, G.; Tan, T.; Cui, Z.; Zhang, X. E. Anal. Chem. 2017, 89, 12896. doi: 10.1021/acs.analchem.7b03584
22. [22]
  Chen, B.; Zou, W.; Xu, H.; Liang, Y.; Huang, B. Nat. Commun. 2018, 9, 1.
23. [23]
  Wang, X.; Xiong, E.; Tian, T.; Cheng, M.; Lin, W.; Wang, H.; Zhang, G.; Sun, J.; Zhou, X. ACS Nano 2020, 14, 2497. doi: 10.1021/acsnano.0c00022
24. [24]
  Xu, W.; Jin, T.; Dai, Y.; Liu, C. C. Biosens. Bioelectron. 2020, 155, 112100. doi: 10.1016/j.bios.2020.112100
25. [25]
  De Ravin, S. S.; Li, L.; Wu, X.; Choi, U.; Allen, C.; Koontz, S.; Lee, J.; Theobald-Whiting, N.; Chu, J.; Garofalo, M.; Sweeney, C.; Kardava, L.; Moir, S.; Viley, A.; Natarajan, P.; Su, L.; Kuhns, D.; Zarember, K. A.; Peshwa, M. V.; Malech, H. L. Sci. Transl. Med. 2017, 9, eaah3480. doi: 10.1126/scitranslmed.aah3480
26. [26]
  Park, H.; Oh, J.; Shim, G.; Cho, B.; Chang, Y.; Kim, S.; Baek, S.; Kim, H.; Shin, J.; Choi, H.; Yoo, J.; Kim, J.; Jun, W.; Lee, M.; Lengner, C. J.; Oh, Y. K.; Kim, J. Nat. Neurosci. 2019, 22, 524. doi: 10.1038/s41593-019-0352-0
27. [27]
  Fu, Y.; Foden, J. A.; Khayter, C.; Maeder, M. L.; Reyon, D.; Joung, J. K.; Sander, J. D. Nat. Biotechnol. 2013, 31, 822. doi: 10.1038/nbt.2623
28. [28]
  Cradick, T. J.; Fine, E. J.; Antico, C. J.; Bao, G. Nucleic Acids Res. 2013, 41, 9584. doi: 10.1093/nar/gkt714
29. [29]
  Lin, Y.; Cradick, T. J.; Brown, M. T.; Deshmukh, H.; Ranjan, P.; Sarode, N.; Wile, B. M.; Vertino, P. M.; Stewart, F. J.; Bao, G. Nucleic Acids Res. 2014, 42, 7473. doi: 10.1093/nar/gku402
30. [30]
  Wu, X.; Scott, D. A.; Kriz, A. J.; Chiu, A. C.; Hsu, P. D.; Dadon, D. B.; Cheng, A. W.; Trevino, A. E.; Konermann, S.; Chen, S.; Jaenisch, R.; Zhang, F.; Sharp, P. A. Nat. Biotechnol. 2014, 32, 670. doi: 10.1038/nbt.2889
31. [31]
  Dominguez, A. A.; Lim, W. A.; Qi, L. S. Nat. Rev. Mol. Cell Biol. 2016, 17, 5. doi: 10.1038/nrm.2015.2
32. [32]
  Knott, G. J.; Doudna, J. A. Science 2018, 361, 866. doi: 10.1126/science.aat5011
33. [33]
  Zhang, X.-H.; Tee, L. Y.; Wang, X.-G.; Huang, Q.-S.; Yang, S.-H. Mol. Ther. Nucleic Acids 2015, 4, e264. doi: 10.1038/mtna.2015.37
34. [34]
  Ran, F. A.; Hsu, P. D.; Lin, C. Y.; Gootenberg, J. S.; Konermann, S.; Trevino, A. E.; Scott, D. A.; Inoue, A.; Matoba, S.; Zhang, Y.; Zhang, F. Cell 2013, 154, 1380. doi: 10.1016/j.cell.2013.08.021
35. [35]
  Fu, Y.; Sander, J. D.; Reyon, D.; Cascio, V. M.; Joung, J. K. Nat. Biotechnol. 2014, 32, 279. doi: 10.1038/nbt.2808
36. [36]
  Guilinger, J. P.; Thompson, D. B.; Liu, D. R. Nat. Biotechnol. 2014, 32, 577. doi: 10.1038/nbt.2909
37. [37]
  Nihongaki, Y.; Otabe, T.; Sato, M. Anal. Chem. 2018, 90, 429. doi: 10.1021/acs.analchem.7b04757
38. [38]
  Cai, W.; Luo, T.; Mao, L; Wang, M. Angew. Chem. Int. Ed. 2020, 10.1002/ange.202005644.
39. [39]
  Hsu, P. D.; Scott, D. A.; Weinstein, J. A.; Ran, F. A.; Konermann, S.; Agarwala, V.; Li, Y.; Fine, E. J.; Wu, X.; Shalem, O.; Cradick, T. J.; Marraffini, L. A.; Bao, G.; Zhang, F. Nat. Biotechnol. 2013, 31, 827. doi: 10.1038/nbt.2647
40. [40]
  Mandegar, M. A.; Huebsch, N.; Frolov, E. B.; Shin, E.; Truong, A.; Olvera, M. P.; Chan, A. H.; Miyaoka, Y.; Holmes, K.; Spencer, C. I.; Judge, L. M.; Gordon, D. E.; Eskildsen, T. V.; Villalta, J. E.; Horlbeck, M. A.; Gilbert, L. A.; Krogan, N. J.; Sheikh, S. P.; Weissman, J. S.; Qi, L. S.; So, P. L.; Conklin, B. R. Cell Stem Cell 2016, 18, 541. doi: 10.1016/j.stem.2016.01.022
41. [41]
  Hoffmann, M. D.; Aschenbrenner, S.; Grosse, S.; Rapti, K.; Domenger, C.; Fakhiri, J.; Mastel, M.; Borner, K.; Eils, R.; Grimm, D.; Niopek, D. Nucleic Acids Res. 2019, 47, e75. doi: 10.1093/nar/gkz271
42. [42]
  Hirosawa, M.; Fujita, Y.; Saito, H. ACS Synth. Biol. 2019, 8, 1575. doi: 10.1021/acssynbio.9b00073
43. [43]
  Hanewich-Hollatz, M. H.; Chen, Z.; Hochrein, L. M.; Huang, J.; Pierce, N. A. ACS Cent. Sci. 2019, 5, 1241. doi: 10.1021/acscentsci.9b00340
44. [44]
  Zetsche, B.; Volz, S. E.; Zhang, F. Nat. Biotechnol. 2015, 33, 139. doi: 10.1038/nbt.3149
45. [45]
  Davis, K. M.; Pattanayak, V.; Thompson, D. B.; Zuris, J. A.; Liu, D. R. Nat. Chem. Biol. 2015, 11, 316. doi: 10.1038/nchembio.1793
46. [46]
  Liu, K. I.; Ramli, M. N.; Woo, C. W.; Wang, Y.; Zhao, T.; Zhang, X.; Yim, G. R.; Chong, B. Y.; Gowher, A.; Chua, M. Z.; Jung, J.; Lee, J. H.; Tan, M. H. Nat. Chem. Biol. 2016, 12, 980. doi: 10.1038/nchembio.2179
47. [47]
  Nguyen, D. P.; Miyaoka, Y.; Gilbert, L. A.; Mayerl, S. J.; Lee, B. H.; Weissman, J. S.; Conklin, B. R.; Wells, J. A. Nat. Commun. 2016, 7, 12009. doi: 10.1038/ncomms12009
48. [48]
  Maji, B.; Moore, C. L.; Zetsche, B.; Volz, S. E.; Zhang, F.; Shoulders, M. D.; Choudhary, A. Nat. Chem. Biol. 2017, 13, 9. doi: 10.1038/nchembio.2224
49. [49]
  Manna, D.; Maji, B.; Gangopadhyay, S. A.; Cox, K. J.; Zhou, Q.; Law, B. K.; Mazitschek, R.; Choudhary, A. Angew. Chem. Int. Ed. 2019, 58, 6285. doi: 10.1002/anie.201900788
50. [50]
  Senturk, S.; Shirole, N. H.; Nowak, D. G.; Corbo, V.; Pal, D.; Vaughan, A.; Tuveson, D. A.; Trotman, L. C.; Kinney, J. B.; Sordella, R. Nat. Commun. 2017, 8, 14370. doi: 10.1038/ncomms14370
51. [51]
  Rose, J. C.; Stephany, J. J.; Valente, W. J.; Trevillian, B. M.; Dang, H. V.; Bielas, J. H.; Maly, D. J.; Fowler, D. M. Nat. Methods 2017, 14, 891. doi: 10.1038/nmeth.4368
52. [52]
  Fontana, J.; Dong, C.; Ham, J. Y.; Zalatan, J. G.; Carothers, J. M. Biotechnol. J. 2018, 13, e1800069. doi: 10.1002/biot.201800069
53. [53]
  Tang, W.; Hu, J. H.; Liu, D. R. Nat. Commun. 2017, 8, 15939. doi: 10.1038/ncomms15939
54. [54]
  Lin, B.; An, Y.; Meng, L.; Zhang, H.; Song, J.; Zhu, Z.; Liu, W.; Song, Y.; Yang, C. Chem. Commun. 2019, 55, 12223. doi: 10.1039/C9CC05531B
55. [55]
  Kundert, K.; Lucas, J. E.; Watters, K. E.; Fellmann, C.; Ng, A. H.; Heineike, B. M.; Fitzsimmons, C. M.; Oakes, B. L.; Qu, J.; Prasad, N.; Rosenberg, O. S.; Savage, D. F.; El-Samad, H.; Doudna, J. A.; Kortemme, T. Nat. Commun. 2019, 10, 2127. doi: 10.1038/s41467-019-09985-2
56. [56]
  Habibian, M.; McKinlay, C.; Blake, T. R.; Kietrys, A. M.; Waymouth, R. M.; Wender, P. A.; Kool, E. T. Chem. Sci. 2020, 11, 1011. doi: 10.1039/C9SC03639C
57. [57]
  Wang, S.-R.; Wu, L.-Y.; Huang, H.-Y.; Xiong, W.; Liu, J.; Wei, L.; Yin, P.; Tian, T.; Zhou, X. Nat. Commun. 2020, 11, 91. doi: 10.1038/s41467-019-13765-3
58. [58]
  Nihongaki, Y.; Kawano, F.; Nakajima, T.; Sato, M. Nat. Biotechnol. 2015, 33, 755. doi: 10.1038/nbt.3245
59. [59]
  Hemphill, J.; Borchardt, E. K.; Brown, K.; Asokan, A.; Deiters, A. J. Am. Chem. Soc. 2015, 137, 5642. doi: 10.1021/ja512664v
60. [60]
  Zhou, X. X.; Zou, X.; Chung, H. K.; Gao, Y.; Liu, Y.; Qi, L. S.; Lin, M. Z. ACS Chem. Biol. 2018, 13, 443. doi: 10.1021/acschembio.7b00603
61. [61]
  Pawluk, A.; Davidson, A. R.; Maxwell, K. L. Nat. Rev. Microbiol. 2018, 16, 12. doi: 10.1038/nrmicro.2017.120
62. [62]
  Bubeck, F.; Hoffmann, M. D.; Harteveld, Z.; Aschenbrenner, S.; Bietz, A.; Waldhauer, M. C.; Borner, K.; Fakhiri, J.; Schmelas, C.; Dietz, L.; Grimm, D.; Correia, B. E.; Eils, R.; Niopek, D. Nat. Methods 2018, 15, 924. doi: 10.1038/s41592-018-0178-9
63. [63]
  Jain, P. K.; Ramanan, V.; Schepers, A. G.; Dalvie, N. S.; Panda, A.; Fleming, H. E.; Bhatia, S. N. Angew. Chem. Int. Ed. 2016, 55, 12440. doi: 10.1002/anie.201606123
64. [64]
  Gao, P.; Pan, W.; Li, N.; Tang, B. Chem. Sci. 2019, 10, 6035. doi: 10.1039/C9SC01652J
65. [65]
  Shao, J.; Wang, M.; Yu, G.; Zhu, S.; Yu, Y.; Heng, B. C.; Wu, J.; Ye, H. Proc. Natl. Acad. Sci. U. S. A. 2018, 115, E6722. doi: 10.1073/pnas.1802448115
66. [66]
  Wang, P.; Zhang, L.; Xie, Y.; Wang, N.; Tang, R.; Zheng, W.; Jiang, X. Adv. Sci. 2017, 4, 1700175. doi: 10.1002/advs.201700175
67. [67]
  Yue, H.; Zhou, X.; Cheng, M.; Xing, D. Nanoscale 2018, 10, 1063. doi: 10.1039/C7NR07999K
68. [68]
  Zhou, W.; Cui, H.; Ying, L.; Yu, X.-F. Angew. Chem. Int. Ed. 2018, 57, 10268. doi: 10.1002/anie.201806941
69. [69]
  Alsaiari, S. K.; Patil, S.; Alyami, M.; Alamoudi, K. O.; Aleisa, F. A.; Merzaban, J. S.; Li, M.; Khashab, N. M. J. Am. Chem. Soc. 2018, 140, 143. doi: 10.1021/jacs.7b11754
70. [70]
  Pan, Y.; Yang, J.; Luan, X.; Liu, X.; Li, X.; Yang, J.; Huang, T.; Sun, L.; Wang, Y.; Lin, Y.; Song, Y. Sci. Adv. 2019, 5, eaav7199. doi: 10.1126/sciadv.aav7199
71. [71]
  Peng, H.; Le, C.; Wu, J.; Li, X. F.; Zhang, H.; Le, X. C. ACS Nano 2020, 14, 2817. doi: 10.1021/acsnano.9b05276
72. [72]
  Wang, P.; Zhang, L.; Zheng, W.; Cong, L.; Guo, Z.; Xie, Y.; Wang, L.; Tang, R.; Feng, Q.; Hamada, Y.; Gonda, K.; Hu, Z.; Wu, X.; Jiang, X. Angew. Chem. Int. Ed. 2018, 57, 1491. doi: 10.1002/anie.201708689
73. [73]
  Chen, X.; Chen, Y.; Xin, H.; Wan, T.; Ping, Y. Proc. Natl. Acad. Sci. U. S. A. 2020, 117, 2395. doi: 10.1073/pnas.1912220117
74. [74]
  Lyu, Y.; He, S.; Li, J.; Jiang, Y.; Sun, H.; Miao, Y.; Pu, K. Angew. Chem. Int. Ed. 2019, 58, 18197. doi: 10.1002/anie.201909264
1. [1]
  Yan Liu , Yang Wang , Jiayi Zhu , Xuxian Su , Xudong Lin , Liang Xu , Xiwen Xing . Employing pH-responsive RNA triplex to control CRISPR/Cas9-mediated gene manipulation in mammalian cells. Chinese Chemical Letters, 2024, 35(9): 109427-. doi: 10.1016/j.cclet.2023.109427
2. [2]
  Kexin Dong , Chuqi Shen , Ruyu Yan , Yanping Liu , Chunqiang Zhuang , Shijie Li . Integration of Plasmonic Effect and S-Scheme Heterojunction into Ag/Ag₃PO₄/C₃N₅ Photocatalyst for Boosted Photocatalytic Levofloxacin Degradation. Acta Physico-Chimica Sinica, 2024, 40(10): 2310013-. doi: 10.3866/PKU.WHXB202310013
3. [3]
  Cheng PENG , Jianwei WEI , Yating CHEN , Nan HU , Hui ZENG . First principles investigation about interference effects of electronic and optical properties of inorganic and lead-free perovskite Cs₃Bi₂X₉ (X=Cl, Br, I). Chinese Journal of Inorganic Chemistry, 2024, 40(3): 555-560. doi: 10.11862/CJIC.20230282
4. [4]
  Xingyang LI , Tianju LIU , Yang GAO , Dandan ZHANG , Yong ZHOU , Meng PAN . A superior methanol-to-propylene catalyst: Construction via synergistic regulation of pore structure and acidic property of high-silica ZSM-5 zeolite. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1279-1289. doi: 10.11862/CJIC.20240026
5. [5]
  Yanhui XUE , Shaofei CHAO , Man XU , Qiong WU , Fufa WU , Sufyan Javed Muhammad . Construction of high energy density hexagonal hole MXene aqueous supercapacitor by vacancy defect control strategy. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1640-1652. doi: 10.11862/CJIC.20240183
6. [6]
  Yan LIU , Jiaxin GUO , Song YANG , Shixian XU , Yanyan YANG , Zhongliang YU , Xiaogang HAO . Exclusionary recovery of phosphate anions with low concentration from wastewater using a CoNi-layered double hydroxide/graphene electronically controlled separation film. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1775-1783. doi: 10.11862/CJIC.20240043
7. [7]
  Xinqiong Li , Guocheng Rao , Xi Peng , Chan Yang , Yanjing Zhang , Yan Tian , Xianghui Fu , Jia Geng . Direct detection of C9orf72 hexanucleotide repeat expansions by nanopore biosensor. Chinese Chemical Letters, 2024, 35(5): 109419-. doi: 10.1016/j.cclet.2023.109419
8. [8]
  Guo-Ping Yin , Ya-Juan Li , Li Zhang , Ling-Gao Zeng , Xue-Mei Liu , Chang-Hua Hu . Citrinsorbicillin A, a novel homotrimeric sorbicillinoid isolated by LC-MS-guided with cytotoxic activity from the fungus Trichoderma citrinoviride HT-9. Chinese Chemical Letters, 2024, 35(8): 109035-. doi: 10.1016/j.cclet.2023.109035
9. [9]
  Jiajun Wang , Guolin Yi , Shengling Guo , Jianing Wang , Shujuan Li , Ke Xu , Weiyi Wang , Shulai Lei . Computational design of bimetallic TM₂@g-C₉N₄ electrocatalysts for enhanced CO reduction toward C₂ products. Chinese Chemical Letters, 2024, 35(7): 109050-. doi: 10.1016/j.cclet.2023.109050

Get Citation

PDF

XML

Metrics

PDF Downloads(17)
Abstract views(1477)
HTML views(220)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142