Citation: Niu Hongyan, Hu Zhengli, Ying Yilun, Long Yi-Tao. Detection of Single c-di-AMP by an Aerolysin Nanopore[J]. Acta Chimica Sinica, ;2019, 77(10): 989-992. doi: 10.6023/A19060230 shu

Detection of Single c-di-AMP by an Aerolysin Nanopore

Niu Hongyan ^a ,
Hu Zhengli ^b ,
Ying Yilun ^a,b,, ,
Long Yi-Tao ^a,b

a.
School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237
b.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023

Corresponding author: Ying Yilun, yilunying@nj.edu.cn
Received Date: 24 June 2019
Available Online: 13 October 2019
Fund Project: the National Ten Thousand Talent Program for Young Top-notch Talent AAAthe National Natural Science Foundation of China 21834001the National Natural Science Foundation of China 61871183the National Natural Science Foundation of China 21922405Project supported by the National Natural Science Foundation of China (Nos. 21922405, 61871183 and 21834001) and the National Ten Thousand Talent Program for Young Top-notch Talent

Figures(3)

Cyclic di-AMP (c-di-AMP) is a ubiquitous second messenger in prokaryotic cells. c-di-AMP can not only effectively regulate various physiological processes such as cell growth, ion transport and cell wall metabolism balance, but also trigger type I interferon response to inspire the body's immune response. Nanopore-based single molecule detection technology is an emerging single molecule detection method which is currently applied to various fields since it has many advantages such as high speed, label-free, high sensitivity and low cost. Aerolysin is a robust biological nanopore with high temporal resolution and high current resolution, which has achieved single oligonucleotide detection, polysaccharide analysis and the studies of enzymolysis kinetics. Aerolysin nanopore is negatively-charged protein nanopore which has numerous negatively charged amino acid residues around its cis entrances. The electrostatic repulsion between the negatively charged c-di-AMP and negatively charged amino acid residues around the cis entrances prevents c-di-AMP entering the nanopore. In this study, 1.0 mol/L LiCl was used as electrolyte solution to facilitate aerolysin analysis of single c-di-AMP molecule. Each event can be characterized by two parameters, the current blockade, I/I₀, and the blockade time, τ_off. The blockades are classified into two populations as PI and PII. The PI events are assigned to c-di-AMP that bump into the pore and then diffuse away. PII events are assigned to traversing of c-di-AMP through the nanopore. Compared with potassium ions, lithium ion can be more effectively to associate with the negative charges on the aerolysin nanopore surface and reduce the electrostatic repulsion between the c-di-AMP molecule and the Aerolysin. The results showed that number of PI events in per minute was significantly increased in 1.0 mol/L LiCl. The number of PI events in per minute in LiCl is 30 times than that in KCl at 90 mV. Hence, Aerolysin nanopore can be used as an ultrasensitive single molecule sensor for cyclic dinucleotides.
- c-di-AMP,
- cyclic dinucleotides,
- aerolysin,
- single molecule interface,
- biological nanopores
1. [1]
  Maelfait, J.; Rehwinkel, J. Immunity 2017, 46, 337. doi: 10.1016/j.immuni.2017.03.005
2. [2]
  Dey, B.; Dey, R. J.; Cheung, L. S.; Pokkali, S.; Guo, H.; Lee, J. H.; Bishai, W. R. Nat. Med. 2015, 21, 401. doi: 10.1038/nm.3813
3. [3]
  Corrigan, R. M.; Campeotto, I.; Jeganathan, T.; Roelofs, K. G.; Lee, V. T.; Gründling, A. Proc. Natl. Acad. Sci. U. S. A. 2013, 110, 9084. doi: 10.1073/pnas.1300595110
4. [4]
  Underwood, A. J.; Zhang, Y.; Metzger, D. W.; Bai, G. J. Microbiol. Meth. 2014, 107, 58. doi: 10.1016/j.mimet.2014.08.026
5. [5]
  Bai, Y.; Yang, J.; Zhou, X.; Ding, X.; Eisele, L. E.; Bai, G. PLoS One 2012, 7, e35206. doi: 10.1371/journal.pone.0035206
6. [6]
  Barker, J. R.; Koestler, B. J.; Carpenter, V. K.; Burdette, D. L.; Waters, C. M.; Vance, R. E.; Valdivia, R. H. mBio. 2013, e00018.
7. [7]
  Kellenberger, C. A.; Wilson, S. C.; Sales-Lee, J.; Hammond, M. C. J. Am. Chem. Soc. 2013, 135, 4906. doi: 10.1021/ja311960g
8. [8]
  Underwood, A. J.; Zhang, Y.; Metzger, D. W.; Bai, G. J. Microbiol. Methods 2014, 107, 58. doi: 10.1016/j.mimet.2014.08.026
9. [9]
  Cao, C.; Long, Y.-T. Acc. Chem. Res. 2018, 5, 331.
10. [10]
  Li, Q.; Lin, Y.; Ying, Y.-L.; Liu, S.-C.; Long, Y.-T. Sci. Sin. Chim. 2017, 47, 1445 (in Chinese).
11. [11]
  Jiang, Y.; Feng, Y.; Su, J.; Nie, J.; Cao, L.; Mao, L.; Jiang, L.; Guo, W. J. Am. Chem. Soc. 2017, 139, 18739. doi: 10.1021/jacs.7b11732
12. [12]
  Qiu, H.; Sarathy, A.; Schulten, K.; Leburton, J. P. npj 2D Mater. Appl. 2017, 1, 3. doi: 10.1038/s41699-017-0005-7
13. [13]
  Sha, J. J.; Shi, H.; Zhang, Y.; Chen, C.; Liu, L.; Chen, Y. ACS Sensors 2017, 2, 506. doi: 10.1021/acssensors.6b00718
14. [14]
  Kasianowicz, J.; Brandin, E.; Branton, D.; Deamer, D. Proc. Natl. Acad. Sci. U. S. A. 1996, 93, 13770. doi: 10.1073/pnas.93.24.13770
15. [15]
  Ying, Y.; Zhang, X.; Liu, Y.; Xue, M.; Li, H.; Long, Y.-T. Acta Chim. Sinica 2013, 71, 44 (in Chinese).
16. [16]
  Ying, Y.-L.; Chao, C.; Hu, Y.-X.; Long, Y.-T. Natl. Sci. Rev. 2018, 5, 450. doi: 10.1093/nsr/nwy029
17. [17]
  Sha, J. J.; Xu, B.; Chen, Y. F.; Yang, Y. J. Acta Chim. Sinica 2017, 75, 1121 (in Chinese).
18. [18]
  Qiu, H.; Girdhar, A.; Schulten, K.; Leburton, J. P. ACS Nano 2016, 10, 4482. doi: 10.1021/acsnano.6b00226
19. [19]
  Parker, M. W.; Buckley, J. T.; Postma, J. P. M.; Tucker, A. D.; Leonard, K.; Pattus, F.; Tsernoglou, D. Nature 1994, 367, 292. doi: 10.1038/367292a0
20. [20]
  Cao, C.; Liao, D. F.; Ying, Y. L.; Long, Y. T. Acta Chim. Sinica 2016, 74, 734 (in Chinese).
21. [21]
  Cao, C.; Ying, Y. L.; Hu, Z. L.; Liao, D. F.; Tian, H.; Long, Y. T. Nat. Nanotech. 2016, 11, 713. doi: 10.1038/nnano.2016.66
22. [22]
  Fennouri, A.; Przybylski, C.; Pastoriza-Gallego, M.; Bacri, L.; Auvray, L.; Daniel, R. ACS Nano 2012, 6, 9672. doi: 10.1021/nn3031047
23. [23]
  Hu, Z.; Du, J.; Ying, Y.; Peng, Y.; Cao, C.; Long, Y.-T. Acta Chim. Sinica 2017, 75, 1087 (in Chinese).
24. [24]
  Xi, D.; Shang, J.; Fan, E.; You, J.; Zhang, S.; Wang, H. Anal. Chem. 2016, 88, 10540. doi: 10.1021/acs.analchem.6b02620
25. [25]
  Cressiot, B.; Braselmann, E.; Oukhaled, A.; Elcock, A. H.; Pelta, J.; Clark, P. L. ACS Nano 2015, 9, 9050. doi: 10.1021/acsnano.5b03053
26. [26]
  Hu, Z. L.; Li, M. Y.; Liu, S. C.; Ying, Y. L.; Long, Y. T. Chem. Sci. 2019, 10, 354. doi: 10.1039/C8SC03927E
27. [27]
  Yang, J.; Li, S.; Wu, X.-Y.; Long, Y.-T. Chin. J. Anal. Chem. 2017, 45, 1766. doi: 10.1016/S1872-2040(17)61053-3
28. [28]
  Iacovache, I.; De Carlo, S.; Cirauqui, N.; Dal Peraro, M.; Van Der Goot, F. G.; Zuber, B. Nat. Commun. 2016, 7, 12062. doi: 10.1038/ncomms12062
29. [29]
  Kowalczyk, S. W.; Wells, D. B.; Aksimentiev, A.; Dekker, C. Nano Lett. 2012, 12, 1038. doi: 10.1021/nl204273h
30. [30]
  Boukhet, M.; Piguet, F.; Ouldali, H.; Pastoriza-Gallego, M.; Pelta, J.; Oukhaled, A. Nanoscale 2016, 8, 18352. doi: 10.1039/C6NR06936C
31. [31]
  Bhattacharya, S.; Muzard, J.; Payet, L.; Mathé, J.; Bockelmann, U.; Aksimentiev, A.; Viasnoff, V. J. Phys. Chem. C 2011, 115, 4255.
32. [32]
  Sutherland, T. C.; Long, Y. T.; Stefureac, R. I.; Bediako-Amoa, I.; Kraatz, H. B.; Lee, J. S. Nano Lett. 2004, 4, 1273. doi: 10.1021/nl049413e
33. [33]
  Stefureac, R.; Long, Y. T.; Kraatz, H. B.; Howard, P.; Lee, J. S. Biochemistry 2006, 45, 9172. doi: 10.1021/bi0604835
34. [34]
  Kasianowicz, J. J.; Brandin, E.; Branton, D.; Deamer, D. W. P. Natl. Acad. Sci. 1996, 93, 13770. doi: 10.1073/pnas.93.24.13770
35. [35]
  Corrigan, R. M.; Abbott, J. C.; Burhenne, H.; Kaever, V.; Gründling, A. PLoS Pathog. 2011, 7, e1002217. doi: 10.1371/journal.ppat.1002217
36. [36]
  Corrigan, R. M.; Bowman, L.; Willis, A. R.; Kaever, V.; Gründling, A. J. Biol. Chem. 2015, 290, 5826. doi: 10.1074/jbc.M114.598300
37. [37]
  Dengler, V.; McCallum, N.; Kiefer, P.; Christen, P.; Patrignani, A.; Vorholt, J. A.; BergerBächi, B.; Senn, M. M. PLoS One 2013, 8, e73512. doi: 10.1371/journal.pone.0073512
38. [38]
  Cao, C.; Liao, D. F.; Yu, J.; Tian, H.; Long, Y. T. Nat. Protoc. 2017, 12, 1901. doi: 10.1038/nprot.2017.077
1. [1]
  Chunmei GUO , Weihan YIN , Jingyi SHI , Jianhang ZHAO , Ying CHEN , Quli FAN . Facile construction and peroxidase-like activity of single-atom platinum nanozyme. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1633-1639. doi: 10.11862/CJIC.20240162
2. [2]
  Yonghui ZHOU , Rujun HUANG , Dongchao YAO , Aiwei ZHANG , Yuhang SUN , Zhujun CHEN , Baisong ZHU , Youxuan ZHENG . Synthesis and photoelectric properties of fluorescence materials with electron donor-acceptor structures based on quinoxaline and pyridinopyrazine, carbazole, and diphenylamine derivatives. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 701-712. doi: 10.11862/CJIC.20230373
3. [3]
  Yuhao SUN , Qingzhe DONG , Lei ZHAO , Xiaodan JIANG , Hailing GUO , Xianglong MENG , Yongmei GUO . Synthesis and antibacterial properties of silver-loaded sod-based zeolite. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 761-770. doi: 10.11862/CJIC.20230169
4. [4]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403
5. [5]
  Yufang GAO , Nan HOU , Yaning LIANG , Ning LI , Yanting ZHANG , Zelong LI , Xiaofeng LI . Nano-thin layer MCM-22 zeolite: Synthesis and catalytic properties of trimethylbenzene isomerization reaction. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1079-1087. doi: 10.11862/CJIC.20240036
6. [6]
  Ruolin CHENG , Haoran WANG , Jing REN , Yingying MA , Huagen LIANG . Efficient photocatalytic CO₂ cycloaddition over W₁₈O₄₉/NH₂-UiO-66 composite catalyst. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 523-532. doi: 10.11862/CJIC.20230349
7. [7]
  Xiaoling LUO , Pintian ZOU , Xiaoyan WANG , Zheng LIU , Xiangfei KONG , Qun TANG , Sheng WANG . Synthesis, crystal structures, and properties of lanthanide metal-organic frameworks based on 2, 5-dibromoterephthalic acid ligand. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1143-1150. doi: 10.11862/CJIC.20230271
8. [8]
  Junke LIU , Kungui ZHENG , Wenjing SUN , Gaoyang BAI , Guodong BAI , Zuwei YIN , Yao ZHOU , Juntao LI . Preparation of modified high-nickel layered cathode with LiAlO₂/cyclopolyacrylonitrile dual-functional coating. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1461-1473. doi: 10.11862/CJIC.20240189
9. [9]
  Juntao Yan , Liang Wei . 2D S-Scheme Heterojunction Photocatalyst. Acta Physico-Chimica Sinica, 2024, 40(10): 2312024-. doi: 10.3866/PKU.WHXB202312024
10. [10]
  Jingjing QING , Fan HE , Zhihui LIU , Shuaipeng HOU , Ya LIU , Yifan JIANG , Mengting TAN , Lifang HE , Fuxing ZHANG , Xiaoming ZHU . Synthesis, structure, and anticancer activity of two complexes of dimethylglyoxime organotin. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1301-1308. doi: 10.11862/CJIC.20240003
11. [11]
  Xiaoning TANG , Shu XIA , Jie LEI , Xingfu YANG , Qiuyang LUO , Junnan LIU , An XUE . Fluorine-doped MnO₂ with oxygen vacancy for stabilizing Zn-ion batteries. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1671-1678. doi: 10.11862/CJIC.20240149
12. [12]
  Peng XU , Shasha WANG , Nannan CHEN , Ao WANG , Dongmei YU . Preparation of three-layer magnetic composite Fe₃O₄@polyacrylic acid@ZiF-8 for efficient removal of malachite green in water. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 544-554. doi: 10.11862/CJIC.20230239
13. [13]
  Jie ZHAO , Sen LIU , Qikang YIN , Xiaoqing LU , Zhaojie WANG . Theoretical calculation of selective adsorption and separation of CO₂ by alkali metal modified naphthalene/naphthalenediyne. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 515-522. doi: 10.11862/CJIC.20230385
14. [14]
  Fan JIA , Wenbao XU , Fangbin LIU , Haihua ZHANG , Hongbing FU . Synthesis and electroluminescence properties of Mn²⁺ doped quasi-two-dimensional perovskites (PEA)₂Pb_yMn_1-yBr₄. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1114-1122. doi: 10.11862/CJIC.20230473
15. [15]
  Xinlong WANG , Zhenguo CHENG , Guo WANG , Xiaokuen ZHANG , Yong XIANG , Xinquan WANG . Enhancement of the fragile interface of high voltage LiCoO₂ by surface gradient permeation of trace amounts of Mg/F. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 571-580. doi: 10.11862/CJIC.20230259
16. [16]
  Xingyang LI , Tianju LIU , Yang GAO , Dandan ZHANG , Yong ZHOU , Meng PAN . A superior methanol-to-propylene catalyst: Construction via synergistic regulation of pore structure and acidic property of high-silica ZSM-5 zeolite. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1279-1289. doi: 10.11862/CJIC.20240026
17. [17]
  Yang YANG , Pengcheng LI , Zhan SHU , Nengrong TU , Zonghua WANG . Plasmon-enhanced upconversion luminescence and application of molecular detection. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 877-884. doi: 10.11862/CJIC.20230440
18. [18]
  Haitang WANG , Yanni LING , Xiaqing MA , Yuxin CHEN , Rui ZHANG , Keyi WANG , Ying ZHANG , Wenmin WANG . Construction, crystal structures, and biological activities of two Ln^Ⅲ₃ complexes. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1474-1482. doi: 10.11862/CJIC.20240188
19. [19]
  Zeyuan WANG , Songzhi ZHENG , Hao LI , Jingbo WENG , Wei WANG , Yang WANG , Weihai SUN . Effect of I₂ interface modification engineering on the performance of all-inorganic CsPbBr₃ perovskite solar cells. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1290-1300. doi: 10.11862/CJIC.20240021
20. [20]
  Xin MA , Ya SUN , Na SUN , Qian KANG , Jiajia ZHANG , Ruitao ZHU , Xiaoli GAO . A Tb₂ complex based on polydentate Schiff base: Crystal structure, fluorescence properties, and biological activity. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1347-1356. doi: 10.11862/CJIC.20230357

Get Citation

PDF

XML

Metrics

PDF Downloads(4)
Abstract views(1303)
HTML views(164)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142