Citation: Zheng Qiao-Feng, Ju Zhen, Liu Shu-Shen. Combined Toxicity of Dichlorvos and Its Metabolites to Vibrio qinghaiensis sp.-Q67 and Caenorhabditis elegans[J]. Acta Chimica Sinica, ;2019, 77(10): 1008-1016. doi: 10.6023/A19060197 shu

Combined Toxicity of Dichlorvos and Its Metabolites to Vibrio qinghaiensis sp.-Q67 and Caenorhabditis elegans

College of Environmental Science and Engineering, Tongji University, Key Laboratory of Yangtze River Water Environment, Ministry of Education, Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092

Corresponding author: Liu Shu-Shen, ssliuhl@263.net
Received Date: 3 June 2019
Available Online: 13 October 2019
Fund Project: the National Natural Science Foundation of China 21677113Project supported by the National Natural Science Foundation of China (Nos. 21437004, 21677113) and the Fundamental Research Funds for the Central Universities (No. 22120180246)the National Natural Science Foundation of China 21437004the Fundamental Research Funds for the Central Universities 22120180246

Figures(5)

Pesticides and their metabolites often coexist in the real environment. The combined toxicity (synergism or antagonism) between pesticide and metabolites directly affects the environment risk assessment of pesticide. Dichlorvos (A) has three main metabolites, 2, 2-dichloroethanol (B), 2, 2-dichloroacetic acid (C) and dimethyl phosphate (D), in water and soil environment. Under different environmental conditions, metabolites with various concentration compositions form a variety of mixtures with dichlorvos. In this paper, five mixture rays with different mixture ratios were selected by optimal experimental design method. A typical aquatic (Vibrio qinghaiensis sp. -Q67) and a soil organisms (Caenorhabditis elegans) were selected as the tested organisms. The photoluminescence inhibitory toxicity (IT) of parent A and its metabolites B, C and D as well as their mixtures to Q67 and the lethal toxicity (LT) to C. elegans at different exposure time and concentration levels were determined by microplate toxicity analysis. The combination index with 95% observation-based confidence intervals was used to evaluate the change of combined toxicity of each mixture ray under different exposure times and the concentration levels. The results showed that the ITs of parent A and two metabolites C and D to Q67 do not change with the exposure time, but the IT of metabolite B at 12 h is significantly larger than that at 0.25 h. However, at two exposure times, the IT of parent A is greater than that of any of metabolites. The LTs of A and B, C and D to C. elegans do not change with the exposure time. The LTs of A, C and D to C. elegans are basically the same and significantly greater than that of B. The ITs of five mixture rays to Q67 at 12 h are significantly greater than those at 0.25 h at various concentration levels. The combined toxicities of the mixture rays to Q67 are concentration additive at low concentration levels and antagonistic at high concentration levels whether at 0.25 h or 12 h. For C. elegans, the LTs of five mixture rays at various concentration levels do not basically change with the exposure time. At two exposure times (12 h and 24 h), the combined toxicities of mixture rays are concentration additive except for the slight antagonism in the rays of R2 and R5.
- pesticide,
- multicomponent mixture,
- uniform design ray,
- basic concentration composition,
- time-dependent toxicity
1. [1]
  Cruz-Alcalde, A.; Sans, C.; Esplugas, S. Sep. Purif. Technol. 2018, 192, 123. doi: 10.1016/j.seppur.2017.09.069
2. [2]
  Park, J. A.; Abd El-Aty, A. M.; Zheng, W.; Kim, S. K.; Cho, S. H.; Choi, J. M.; Hacimuftuo, A.; Jeong, J. H.; Wang, J.; Shim, J. H.; Shin, H. C. Food Chem. 2018, 252, 40. doi: 10.1016/j.foodchem.2018.01.085
3. [3]
  Yu, Y.; Hu, S.; Yang, Y.; Zhao, X.; Xue, J.; Zhang, J.; Gao, S.; Yang, A. BMC Public Health 2017, 18, 91.
4. [4]
  Dirican, E. K.; Kalender, Y. Exp. Toxicol. Pathol. 2012, 64, 821. doi: 10.1016/j.etp.2011.03.002
5. [5]
  Oribhabor, B. J.; Ikeogu, G. C. Recent Pat. Biotechnol. 2016, 10, 272. doi: 10.2174/1872208310666160725200722
6. [6]
  Oncescu, T.; Oancea, P.; Enache, M.; Popescu, G.; Dumitru, L.; Kamekura, M. Cent. Eur. J. Biol. 2007, 2, 563. doi: 10.2478/s11535-007-0037-7
7. [7]
  Barrett, K.; Jaward, F. M. Int. J. Environ. Health Res. 2012, 22, 481. doi: 10.1080/09603123.2012.667794
8. [8]
  Boxall, A. B. A.; Sinclair, C. J.; Fenner, K.; Kolpin, D.; Maud, S. J. Environ. Sci. Technol. 2004, 38, 368A. doi: 10.1021/es040624v
9. [9]
  Benfeito, S.; Silva, T.; Garrido, J.; Andrade, P. B.; Sottomayor, M. J.; Borges, F.; Garrido, E. M. Biomed. Res. Int. 2014, no. 709036 (DOI: 10.1155/2014/709036). doi: 10.1155/2014/709036)
10. [10]
  Zhang, Q.; Ji, C.; Yan, L.; Lu, M.; Lu, C.; Zhao, M. Environ. Pollut. 2016, 218, 8. doi: 10.1016/j.envpol.2016.08.026
11. [11]
  Stancova, V.; Plhalova, L.; Bartoskova, M.; Zivna, D.; Prokes, M.; Marsalek, P.; Blahova, J.; Skoric, M.; Svobodova, Z. Biomed. Res. Int. 2014, no. 253468 (DOI: 10.1155/2014/253468). doi: 10.1155/2014/253468)
12. [12]
  Gatidou, G.; Thomaidis, N. S. Aquat. Toxicol. 2007, 85, 184. doi: 10.1016/j.aquatox.2007.09.002
13. [13]
  Liu, S. S.; Xiao, Q. F.; Zhang, J.; Yu, M. Sci. Bull. 2016, 61, 52. doi: 10.1007/s11434-015-0925-6
14. [14]
  Moser, V. C.; Simmons, J. E.; Gennings, C. Toxicol. Sci. 2006, 92, 235. doi: 10.1093/toxsci/kfj189
15. [15]
  Stork, L. G.; Gennings, C.; Carter, W. H., Jr.; Johnson, R. E.; Mays, D. P.; Simmons, J. E.; Wagner, E. D.; Plewa, M. J. J. Agric. Biol. Environ. Stat. 2007, 12, 514. doi: 10.1198/108571107X249816
16. [16]
  Fang, K. T.; Lin, D. K. J.; Winker, P.; Zhang, Y. Technometrics 2000, 42, 237. doi: 10.1080/00401706.2000.10486045
17. [17]
  Chou, T. C. Pharmacol. Rev. 2006, 58, 621. doi: 10.1124/pr.58.3.10
18. [18]
  Li, K.; Liu, S. S.; Qu, R. Asian J. Ecotoxicol. 2017, 12, 62 (in Chinese). doi: 10.7524/AJE.1673-5897.20160712001
19. [19]
  Feng, L.; Liu, S. S.; Li, K.; Tang, H. X.; Liu, H. L. J. Hazard. Mater. 2017, 327, 11. doi: 10.1016/j.jhazmat.2016.12.031
20. [20]
  Tang, H. X.; Liu, S. S.; Li, K.; Feng, L. Anal. Methods 2016, 8, 4466. doi: 10.1039/C6AY00582A
21. [21]
  Liu, L.; Liu, S. S.; Yu, M.; Chen, F. Environ. Toxicol. Pharmacol. 2015, 39, 447. doi: 10.1016/j.etap.2014.12.013
22. [22]
  Chen, Y. H.; Qin, L. T.; Mo, L. Y.; Zhao, D. N.; Zeng, H. H.; Liang, Y. P. Environ. Pollut. 2019, 250, 375. doi: 10.1016/j.envpol.2019.04.009
23. [23]
  Girotti, S.; Ferri, E. N.; Fumo, M. G.; Maiolini, E. Anal. Chim. Acta 2008, 608, 2. doi: 10.1016/j.aca.2007.12.008
24. [24]
  Wu, S.; Zhang, X.; Yang, P.; Li, L.; Tang, S. Int. J. Food Sci. Technol. 2018, 53, 2141. doi: 10.1111/ijfs.13801
25. [25]
  Jian, Q.; Gong, L.; Li, T.; Wang, Y.; Wu, Y.; Chen, F.; Qu, H.; Duan, X.; Jiang, Y. Toxins (Basel) 2017, 9, 335. doi: 10.3390/toxins9100335
26. [26]
  Thomulka, K. W.; McGee, D. J.; Lange, J. H. Bull. Environ. Contam. Toxicol. 1993, 51, 538. doi: 10.1007/BF00192169
27. [27]
  Gong, L.; Wu, Y.; Jian, Q.; Yin, C.; Li, T.; Gupta, V. K.; Duan, X.; Jiang, Y. Sci. Data 2018, 5, 170105.
28. [28]
  Ding, K.; Lu, L.; Wang, J.; Wang, J.; Zhou, M.; Zheng, C.; Liu, J.; Zhang, C.; Zhuang, S. Sci. Total Environ. 2017, 580, 1078. doi: 10.1016/j.scitotenv.2016.12.062
29. [29]
  Fan, Y.; Liu, S. S.; Qu, R.; Li, K.; Liu, H. L. RSC Adv. 2017, 7, 6080. doi: 10.1039/C6RA25843C
30. [30]
  Zhang, J.; Ding, T. T.; Dong, X. Q.; Bian, Z. Q. RSC Adv. 2018, 8, 26089. doi: 10.1039/C8RA04191A
31. [31]
  Mo, L. Y.; Liu, J.; Qin, L. T.; Zeng, H. H.; Liang, Y. P. Bull. Environ. Contam. Toxicol. 2017, 99, 17. doi: 10.1007/s00128-017-2099-1
32. [32]
  Xu, Y. Q.; Liu, S. S.; Wang, Z. J.; Li, K.; Qu, R. Ecotoxicol. Environ. Saf. 2018, 162, 304. doi: 10.1016/j.ecoenv.2018.07.007
33. [33]
  Zhou, D.; Yang, J.; Li, H.; Cui, C.; Yu, Y.; Liu, Y.; Lin, K. Chemosphere 2016, 154, 546. doi: 10.1016/j.chemosphere.2016.04.011
34. [34]
  Jager, T.; Gudmundsdottir, E. M.; Cedergreen, N. Environ. Sci. Technol. 2014, 48, 7026. doi: 10.1021/es501306t
35. [35]
  Cole, R. D.; Anderson, G. L.; Williams, P. L. Toxicol. Appl. Pharmacol. 2004, 194, 248. doi: 10.1016/j.taap.2003.09.013
36. [36]
  Yu, Z.; Yin, D.; Deng, H. Ecotoxicol. Environ. Saf. 2015, 111, 66. doi: 10.1016/j.ecoenv.2014.09.026
37. [37]
  Sochova, I.; Hofman, J.; Holoubek, I. Environ. Int. 2007, 33, 798. doi: 10.1016/j.envint.2007.03.001
38. [38]
  Varo, I.; Perini, A.; Torreblanca, A.; Garcia, Y.; Bergami, E.; Vannuccini, M. L.; Corsi, I. Sci. Total Environ. 2019, 675, 570. doi: 10.1016/j.scitotenv.2019.04.157
39. [39]
  Li, T.; Liu, S. S.; Qu, R.; Liu, H. L. Ecotoxicol. Environ. Saf. 2017, 144, 475. doi: 10.1016/j.ecoenv.2017.06.044
40. [40]
  Yu, Z. Y.; Mo, L. Y.; Zhang, J.; Liu, S. S. Chemosphere 2016, 163, 452. doi: 10.1016/j.chemosphere.2016.08.061
41. [41]
  Li, K.; Xu, Y. Q.; Feng, L.; Liu, S. S. Environ. Pollut. 2018, 242, 872. doi: 10.1016/j.envpol.2018.06.107
42. [42]
  Zhang, J.; Wang, J. C.; Liu, S. S.; Ge, H. L.; Liu, H. L. Asian J. Ecotoxicol. 2009, 4, 353 (in Chinese).
43. [43]
  Zhang, J.; Liu, S. S. Asian J. Ecotoxicol. 2012, 7, 408 (in Chinese).
44. [44]
  Zhang, J.; Liu, S. S.; Zhu, X. W. Chemosphere 2014, 112, 420. doi: 10.1016/j.chemosphere.2014.05.007
45. [45]
  Evgenidou, E.; Konstantinou, I.; Fytianos, K.; Albanis, T. J. Hazard. Mater. 2006, 137, 1056. doi: 10.1016/j.jhazmat.2006.03.042
46. [46]
  Xu, Y. Q.; Liu, S. S.; Fan, Y.; Li, K. Sci. Total Environ. 2018, 635, 432. doi: 10.1016/j.scitotenv.2018.04.023
47. [47]
  Zhang, J.; Liu, S. S.; Yu, Z. Y.; Zhang, J. Chemosphere 2013, 91, 462. doi: 10.1016/j.chemosphere.2012.11.070
48. [48]
  Zhu, X. W.; Liu, S. S.; Ge, H. L.; Liu, Y. Water Res. 2009, 43, 1731. doi: 10.1016/j.watres.2009.01.004
49. [49]
  Wang, M. C.; Liu, S. S.; Chen, F. Acta Chim. Sinica 2014, 72, 56 (in Chinese).
50. [50]
  Zhu, X. W.; Liu, S. S.; Ge, H. L.; Liu, Y. China Environ. Sci. 2009, 29, 113 (in Chinese). doi: 10.3321/j.issn:1000-6923.2009.02.001
51. [51]
  Zhu, X. W.; Liu, S. S.; Qin, L. T.; Chen, F.; Liu, H. L. Ecotoxicol. Environ. Saf. 2013, 89, 130. doi: 10.1016/j.ecoenv.2012.11.022
52. [52]
  Liu, S. S.; Zhang, J.; Zhang, Y. H.; Qin, L. T. Acta Chim. Sinica 2012, 70, 1511 (in Chinese). doi: 10.3969/j.issn.0251-0790.2012.07.027
53. [53]
  Zhang, Y. H.; Liu, S. S.; Liu, H. L.; Liu, Z. Z. Pest Manag. Sci. 2010, 66, 879. doi: 10.1002/ps.1957
1. [1]
  Shahua Huang , Xiaoming Guo , Lin Lin , Guangping Chang , Sheng Han , Zuxin Zhou . Application of “Integration of Industry and Education” in Engineering Chemistry: Improvement of the Pesticide Fipronil Production. University Chemistry, 2024, 39(3): 199-204. doi: 10.3866/PKU.DXHX202309064
2. [2]
  Cunling Ye , Xitong Zhao , Hongfang Wang , Zhike Wang . A Formula for the Calculation of Complex Concentrations Arising from Side Reactions and Its Applications. University Chemistry, 2024, 39(4): 382-386. doi: 10.3866/PKU.DXHX202310043
3. [3]
  Qilong Fang , Yiqi Li , Jiangyihui Sheng , Quan Yuan , Jie Tan . Magical Pesticide Residue Detection Test Strips: Aptamer-based Lateral Flow Test Strips for Organophosphorus Pesticide Detection. University Chemistry, 2024, 39(5): 80-89. doi: 10.3866/PKU.DXHX202310004
4. [4]
  Xuan Zhou , Yi Fan , Zhuoqi Jiang , Zhipeng Li , Guowen Yuan , Laiying Zhang , Xu Hou . Liquid Gating Mechanism and Basic Properties Characterization: a New Experimental Design for Interface and Surface Properties in the Chemistry “101 Plan”. University Chemistry, 2024, 39(10): 113-120. doi: 10.12461/PKU.DXHX202407111
5. [5]
  Lijun Dong , Pengcheng Du , Guangnong Lu , Wei Wang . Exploration and Practice of Independent Design Experiments in Inorganic and Analytical Chemistry: A Case Study of “Preparation and Composition Analysis of Tetraammine Copper(II) Sulfate”. University Chemistry, 2024, 39(4): 361-366. doi: 10.3866/PKU.DXHX202310041
6. [6]
  Yan LIU , Jiaxin GUO , Song YANG , Shixian XU , Yanyan YANG , Zhongliang YU , Xiaogang HAO . Exclusionary recovery of phosphate anions with low concentration from wastewater using a CoNi-layered double hydroxide/graphene electronically controlled separation film. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1775-1783. doi: 10.11862/CJIC.20240043
7. [7]
  Liang TANG , Jingfei NI , Kang XIAO , Xiangmei LIU . Synthesis and X-ray imaging application of lanthanide-organic complex-based scintillators. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1892-1902. doi: 10.11862/CJIC.20240139
8. [8]
  Ruoxi Sun , Yiqian Xu , Shaoru Rong , Chunmiao Han , Hui Xu . The Enchanting Collision of Light and Time Magic: Exploring the Footprints of Long Afterglow Lifetime. University Chemistry, 2024, 39(5): 90-97. doi: 10.3866/PKU.DXHX202310001
9. [9]
  Hui Shi , Shuangyan Huan , Yuzhi Wang . Ideological and Political Design of Potassium Permanganate Oxidation-Reduction Titration Experiment. University Chemistry, 2024, 39(2): 175-180. doi: 10.3866/PKU.DXHX202308042
10. [10]
  Wei Peng , Baoying Wen , Huamin Li , Yiru Wang , Jianfeng Li . Exploration and Practice on Raman Scattering Spectroscopy Experimental Teaching. University Chemistry, 2024, 39(8): 230-240. doi: 10.3866/PKU.DXHX202312062
11. [11]
  Dongju Zhang . Exploring the Descriptions and Connotations of Basic Concepts of Teaching Crystal Structures. University Chemistry, 2024, 39(3): 18-22. doi: 10.3866/PKU.DXHX202304003
12. [12]
  Zhen Yao , Bing Lin , Youping Tian , Tao Li , Wenhui Zhang , Xiongwei Liu , Wude Yang . Visible-Light-Mediated One-Pot Synthesis of Secondary Amines and Mechanistic Exploration. University Chemistry, 2024, 39(5): 201-208. doi: 10.3866/PKU.DXHX202311033
13. [13]
  Ruilin Han , Xiaoqi Yan . Comparison of Multiple Function Methods for Fitting Surface Tension and Concentration Curves. University Chemistry, 2024, 39(7): 381-385. doi: 10.3866/PKU.DXHX202311023
14. [14]
  Wei Li , Ze Chang , Meihui Yu , Ying Zhang . Curriculum Ideological and Political Design of Piezoelectricity Measurement Experiments of Coordination Compounds. University Chemistry, 2024, 39(2): 77-82. doi: 10.3866/PKU.DXHX202308004
15. [15]
  Zhihuan XU , Qing KANG , Yuzhen LONG , Qian YUAN , Cidong LIU , Xin LI , Genghuai TANG , Yuqing LIAO . Effect of graphene oxide concentration on the electrochemical properties of reduced graphene oxide/ZnS. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1329-1336. doi: 10.11862/CJIC.20230447
16. [16]
  Jinfeng Chu , Yicheng Wang , Ji Qi , Yulin Liu , Yan Li , Lan Jin , Lei He , Yufei Song . Comprehensive Chemical Experiment Design: Convenient Preparation and Characterization of an Oxygen-Bridged Trinuclear Iron(III) Complex. University Chemistry, 2024, 39(7): 299-306. doi: 10.3866/PKU.DXHX202310105
17. [17]
  Zhening Lou , Quanxing Mao , Xiaogeng Feng , Lei Zhang , Xu Xu , Yuyang Zhang , Xueyan Liu , Hongling Kang , Dongyang Feng , Yongku Li . Practice of Implementing Blended Teaching in Shared Analytical Chemistry Course. University Chemistry, 2024, 39(2): 263-269. doi: 10.3866/PKU.DXHX202308089
18. [18]
  Xu Liu , Chengfang Liu , Jie Huang , Xiangchun Li , Wenyong Lai . Research on the Application of Diversified Teaching Models in the Teaching of Physical Chemistry. University Chemistry, 2024, 39(8): 112-118. doi: 10.3866/PKU.DXHX202402021
19. [19]
  Yuan Zheng , Quan Lan , Zhenggen Zha , Lingling Li , Jun Jiang , Pingping Zhu . Teaching Reform of Organic Synthesis Experiments by Introducing Reverse Thinking and Design Concepts: Taking the Synthesis of Cinnamic Acid Based on Retrosynthetic Analysis as an Example. University Chemistry, 2024, 39(6): 207-213. doi: 10.3866/PKU.DXHX202310065
20. [20]
  Caixia Lin , Zhaojiang Shi , Yi Yu , Jianfeng Yan , Keyin Ye , Yaofeng Yuan . Ideological and Political Design for the Electrochemical Synthesis of Benzoxathiazine Dioxide Experiment. University Chemistry, 2024, 39(2): 61-66. doi: 10.3866/PKU.DXHX202309005

Get Citation

PDF

XML

Metrics

PDF Downloads(7)
Abstract views(1388)
HTML views(162)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142