Citation: Zhu Shuihong, Luo Wenhao, Zeng Wenbin, Lin Youhui, Liu Xiang Yang. Preparation of Free-standing Micropatterned Keratin Films by Soft Lithography[J]. Acta Chimica Sinica, ;2019, 77(6): 533-538. doi: 10.6023/A19020060 shu

Preparation of Free-standing Micropatterned Keratin Films by Soft Lithography

a.
Department of Physics, Research Institute for Biomimetics and Soft Matter, Fujian Provincial Key Laboratory for Soft Functional Materials Research, Xiamen University, Xiamen 361005, China
b.
Department of Physics, National University of Singapore, 2 Science Drive 3, Singapore, 117542

Corresponding author: Lin Youhui, linyouhui@xmu.edu.cn Liu Xiang Yang, phyliuxy@nus.edu.sg
Received Date: 11 February 2019
Available Online: 29 June 2019
Fund Project: the National Natural Science Foundation of China 21401154the National Natural Science Foundation of China 21771150the 111 Project B16029the National Natural Science Foundation of China U1405226Project supported by the National Natural Science Foundation of China (Nos. 21771150, 21401154, U1405226), the 111 Project (No. B16029), the Natural Science Foundation of Guangdong Province (2014A030310005) and the Fundamental Research Funds for the Central Universities of China (No. 20720170011)the Fundamental Research Funds for the Central Universities of China 20720170011the Natural Science Foundation of Guangdong Province 2014A030310005

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Recently, the use of micro-nano manufacturing processes to fabricate high-precision spatial patterns of proteins or peptides has provided important applications in cell biology, tissue engineering, pharmaceutical science, and optoelectronics. As a natural biological protein, wool keratin (WK) have excellent water solubility, good biocompatibility, and controllable degradability. However, WK usually cannot self-assemble to form a gel network or other insoluble forms. Therefore, it is difficult to prepare molded WK materials, such as a fiber, a film, and a gel. To solve this problem, this paper explores the feasibility of preparing photocrosslinkable WK. WK was extracted from wool fibres, and its side groups were reacted with the reagent 2-isocyanatoethyl methacrylate (IEM), yielding a photoactive WK precursor. And then, WK films with patterned microstructures were obtained by a covalent cross-linking method. This method can also be used to obtain other forms of WK materials. The as-prepared WK films were characterized by 3D laser scanning microscopy, UV-visible near-infrared spectroscopy and Fourier transform infrared microscopy. The experimental results showed that after two pattern shifts, the pattern on the WK film still maintained good integrity and conformed to the original pattern on the silicon wafer, which indicated that the pattern transfer method can achieve perfect reproduction of the pattern. In addition, we also demonstrated that the formation of structural colors caused by periodically arranged microstructures on WK films. Our experimental results not only provide a facile method to prepare WK films with surface microstructures by soft lithography but also give a new way for the preparation of molded WK. We expect the good optical properties and controlled degradation properties of WK open up new directions for the manufacture of biodegradable optics and implantable flexible microelectronic devices.
- wool keratin,
- soft lithography,
- covalent cross-linking,
- patterns,
- surface microstructure
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