Citation: Feng Tingting, Gao Shouqin, Wang Kun. Colorimetric Sensing of Prostate Specific Membrane Antigen Based on Gold Nanoparticles[J]. Acta Chimica Sinica, ;2019, 77(5): 422-426. doi: 10.6023/A19010018 shu

Colorimetric Sensing of Prostate Specific Membrane Antigen Based on Gold Nanoparticles

College of Traditional Chinese Medicine, Shanxi University of Chinese Medicine, Jinzhong 030619

Corresponding author: Feng Tingting, tingtingfeng1985@126.com
Received Date: 9 January 2019
Available Online: 26 May 2019
Fund Project: the Doctoral Research Start-up Fund of Shanxi University of Chinese Medicine 2014BL19Project supported by the Doctoral Research Start-up Fund of Shanxi University of Chinese Medicine (No. 2014BL19)

Figures(6)

Cancer is a major cause of death and its early diagnosis has been a research goal for many decades. For males, prostatic carcinoma has become the second leading cause of cancer death worldwide. Prostate specific membrane antigen (PSMA) has been widely recognized as a prostate cancer marker. Thus, measurement of PSMA would be more valuable for the early diagnosis of prostate cancer. Nanomaterials have the characteristics of small size effect, quantum size effect, macroscopic quantum tunneling effect and surface effect, and have been widely used in various fields, such as cell imaging, analysis and detection, drug release and treatment. Gold nanoparticles have been widely used in biosensing and medical diagnosis due to their simple preparation, high stability and unique photoelectric properties. In this paper, a new colorimetric approach is proposed for simple detection of PSMA based on gold nanoparticles. In the experiment, we synthesized gold nanoparticles with positive charges, and the polyanionic peptide as the substrate of PSMA. The detection of PSMA was based on the property that different aggregation states of gold nanoparticles can lead to the change of color and the specific recognition of PSMA for its substrate. The positively charged gold nanoparticles interact electrostatically with polyanionic peptide, resulting in aggregation of gold nanoparticles. In the presence of PSMA, however, the polyanionic peptide are hydrolyzed into glutamic acid fragment due to the reaction between the PSMA and the polyanionic peptide, resulting in the dispersion of gold nanoparticles. This behaviour leads to the development of a rapid and simple colorimetric method for assaying PSMA activity, with a detection limit of 0.5 nmol/L and the linear range of 2~10 nmol/L. This approach is simple compared to the existing ones since the gold nanoparticles-peptide based sensor is easy to be assembled and the detection can be achieved without the involvement of complicated procedures. Moreover, the applicability of the method has been demonstrated by detecting PSMA spiked into urine samples.
- gold nanoparticles,
- colorimetric sensing system,
- prostate-specific membrane antigen,
- specific recognition,
- peptide
1. [1]
  Juzgado, A.; Soldà, A.; Ostric, A.; Criado, A.; Valenti, G.; Rapino, S.; Conti, G.; Fracasso, G.; Paolucci, F.; Prato, M. J. Mater. Chem. B 2017, 5, 6681. doi: 10.1039/C7TB01557G
2. [2]
  Huang, W. F.; Chang, C. L.; Brault, N. D.; Gur, O.; Wang, Z.; Jalal, S. I.; Low, P. S.; Ratliff, T. L.; Pili, R.; Savran, C. A. Lab Chip. 2017, 17, 415. doi: 10.1039/C6LC01279E
3. [3]
  Ferraris, D. V.; Shukla, K.; Tsukamoto, T. Curr. Med. Chem. 2012, 19, 1282. doi: 10.2174/092986712799462658
4. [4]
  Yang, H. W.; Hua, M. Y.; Liu, H. L.; Tsai, R. Y.; Chuang, C. K.; Chu, P. C.; Wu, P. Y.; Chang, Y. H.; Chuang, H. C.; Yu, K. J.; Pang, S. T. ACS Nano 2012, 6, 1795. doi: 10.1021/nn2048526
5. [5]
  Pu, F.; Salarian, M.; Xue, S. H.; Qiao, J. J.; Feng, J.; Tan, S. S.; Patel, A.; Li, X.; Mamouni, K.; Hekmatyar, K.; Zou, J.; Wu, D. Q.; Yang, J. J. Nanoscale 2016, 8, 12668. doi: 10.1039/C5NR09071G
6. [6]
  Min, K.; Song, K. M.; Cho, M.; Chun, Y. S.; Shim, Y. B.; Ku, J. K.; Ban, C. Chem. Commun. 2010, 46, 5566. doi: 10.1039/c002524k
7. [7]
  Carter, R. E.; Feldman, A. R.; Coyle, J. T. Proc. Natl. Acad. Sci. U. S. A. 1996, 93, 749. doi: 10.1073/pnas.93.2.749
8. [8]
  Kamga, I.; Ng, R.; Hosaka, M.; Berkman, C. E. Anal. Biochem. 2002, 310, 125. doi: 10.1016/S0003-2697(02)00284-1
9. [9]
  Wang, H. S.; Lin, P. T.; Zhao, S. L.; Li, S. T.; Lu, X.; Liang, H. Chin. J. Chem. 2017, 35, 943. doi: 10.1002/cjoc.v35.6
10. [10]
  Yang, L. M.; Liu, B.; Li, N.; Tang, B. Acta Chim. Sinica 2017, 75, 1047.
11. [11]
  Li, M. B.; Tian, S. K.; Wu, Z. K. Chin. J. Chem. 2017, 35, 567. doi: 10.1002/cjoc.v35.5
12. [12]
  Li, S. S.; Wang, F. Z. R.; Liu, Y. M.; Cao, Y. Chin. J. Chem. 2017, 35, 591. doi: 10.1002/cjoc.v35.5
13. [13]
  Zhu, A.W.; Qu, Q.; Shao, X. L.; Kong, B.; Tian, Y. Angew. Chem., Int. Ed. 2012, 51, 7185. doi: 10.1002/anie.201109089
14. [14]
  Zhang, Y. Y.; Wu, M. H.; Wu, M. J.; Guo, L. P.; Cao, L., Wu, H. Y.; Zhang, X. N. Acta Chim. Sinica 2018, 76, 709.
15. [15]
  Ji, G.; Yan, L. L.; Wang, H.; Ma, L.; Xu, B.; Tian, W. J. Acta Chim. Sinica 2016, 74, 917.
16. [16]
  Men, J. Y.; Gao, B. J.; Chen, Z. P.; Yao, L. Acta Chim. Sinica 2012, 70, 2273.
17. [17]
  Wen, Q. S.; Tang, H. W.; Yang, G. M.; Liu, L. B.; Lv, F. T.; Yang, Q.; Wang, S. Acta Chim. Sinica 2012, 70, 2137.
18. [18]
  Elghanian, R.; Storhoff, J. J.; Mucic, R. C.; Letsinger, R. L.; Mirkin, C. A. Science 1997, 277, 1078. doi: 10.1126/science.277.5329.1078
19. [19]
  Taton, T. A.; Mirkin, C. A.; Letsinger, R. L. Science 2000, 289, 1757. doi: 10.1126/science.289.5485.1757
20. [20]
  Fratoddi, I.; Venditti, I.; Cametti, C.; Russo, M. V. J. Mater. Chem. B 2014, 2, 4204. doi: 10.1039/C4TB00383G
21. [21]
  Obare, S. O.; Hollowell, R. E.; Murphy, C. J. Langmuir 2002, 18, 10407. doi: 10.1021/la0260335
22. [22]
  Kim, Y.; Johnson, R. C.; Hupp, J. T. Nano Lett. 2001, 1, 165. doi: 10.1021/nl0100116
23. [23]
  Zhou, Y.; Wang, S.; Zhang, K.; Jiang, X. Angew. Chem., Int. Ed. 2008, 47, 7454. doi: 10.1002/anie.v47:39
24. [24]
  Anderson, M. O.; Wu, L. Y.; Santiago, N. M. Bioorgan. Med. Chem. 2007, 15, 6678. doi: 10.1016/j.bmc.2007.08.006
25. [25]
  Feng, D.; Zhang, Y. Y.; Feng, T. T.; Shi, W.; Li, X. H.; Ma, H. M. Chem. Commun. 2011, 47, 10680. doi: 10.1039/c1cc13975d
26. [26]
  Miao, X. M.; Cheng, Z. Y.; Li, Z. B.; Wang, P. Biochem. Eng. J. 2017, 117, 21. doi: 10.1016/j.bej.2016.10.022
27. [27]
  Sun, C. D.; Shi, W.; Song, Y. C.; Chen, W.; Ma, H. M. Chem. Commun. 2011, 47, 8638. doi: 10.1039/c1cc12174j
28. [28]
  Mohan, K. M.; Donavan, K. C.; Arter, J. A.; Penner, R. M.; Weiss, G. A. J. Am. Chem. Soc. 2013, 135, 7761. doi: 10.1021/ja4028082
29. [29]
  Li, C. M.; Li, Y. F.; Wang, J.; Huang, C. Z. Talanta 2010, 81, 1339.
1. [1]
  Hong LI , Xiaoying DING , Cihang LIU , Jinghan ZHANG , Yanying RAO . Detection of iron and copper ions based on gold nanorod etching colorimetry. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 953-962. doi: 10.11862/CJIC.20230370
2. [2]
  Yan LIU , Jiaxin GUO , Song YANG , Shixian XU , Yanyan YANG , Zhongliang YU , Xiaogang HAO . Exclusionary recovery of phosphate anions with low concentration from wastewater using a CoNi-layered double hydroxide/graphene electronically controlled separation film. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1775-1783. doi: 10.11862/CJIC.20240043
3. [3]
  Jingke LIU , Jia CHEN , Yingchao HAN . Nano hydroxyapatite stable suspension system: Preparation and cobalt adsorption performance. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1763-1774. doi: 10.11862/CJIC.20240060
4. [4]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403
5. [5]
  Qin ZHU , Jiao MA , Zhihui QIAN , Yuxu LUO , Yujiao GUO , Mingwu XIANG , Xiaofang LIU , Ping NING , Junming GUO . Morphological evolution and electrochemical properties of cathode material LiAl_0.08Mn_1.92O₄ single crystal particles. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1549-1562. doi: 10.11862/CJIC.20240022
6. [6]
  Tiantian MA , Sumei LI , Chengyu ZHANG , Lu XU , Yiyan BAI , Yunlong FU , Wenjuan JI , Haiying YANG . Methyl-functionalized Cd-based metal-organic framework for highly sensitive electrochemical sensing of dopamine. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 725-735. doi: 10.11862/CJIC.20230351
7. [7]
  Lu XU , Chengyu ZHANG , Wenjuan JI , Haiying YANG , Yunlong FU . Zinc metal-organic framework with high-density free carboxyl oxygen functionalized pore walls for targeted electrochemical sensing of paracetamol. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 907-918. doi: 10.11862/CJIC.20230431
8. [8]
  Jing SU , Bingrong LI , Yiyan BAI , Wenjuan JI , Haiying YANG , Zhefeng Fan . Highly sensitive electrochemical dopamine sensor based on a highly stable In-based metal-organic framework with amino-enriched pores. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1337-1346. doi: 10.11862/CJIC.20230414
9. [9]
  Peiran ZHAO , Yuqian LIU , Cheng HE , Chunying DUAN . A functionalized Eu³⁺ metal-organic framework for selective fluorescent detection of pyrene. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 713-724. doi: 10.11862/CJIC.20230355
10. [10]
  Jinlong YAN , Weina WU , Yuan WANG . A simple Schiff base probe for the fluorescent turn-on detection of hypochlorite and its biological imaging application. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1653-1660. doi: 10.11862/CJIC.20240154
11. [11]
  Guimin ZHANG , Wenjuan MA , Wenqiang DING , Zhengyi FU . Synthesis and catalytic properties of hollow AgPd bimetallic nanospheres. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 963-971. doi: 10.11862/CJIC.20230293
12. [12]
  Yuhao SUN , Qingzhe DONG , Lei ZHAO , Xiaodan JIANG , Hailing GUO , Xianglong MENG , Yongmei GUO . Synthesis and antibacterial properties of silver-loaded sod-based zeolite. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 761-770. doi: 10.11862/CJIC.20230169
13. [13]
  Wenjiang LI , Pingli GUAN , Rui YU , Yuansheng CHENG , Xianwen WEI . C₆₀-MoP-C nanoflowers van der Waals heterojunctions and its electrocatalytic hydrogen evolution performance. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 771-781. doi: 10.11862/CJIC.20230289
14. [14]
  Peng ZHOU , Xiao CAI , Qingxiang MA , Xu LIU . Effects of Cu doping on the structure and optical properties of Au₁₁(dppf)₄Cl₂ nanocluster. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1254-1260. doi: 10.11862/CJIC.20240047
15. [15]
  Siyu HOU , Weiyao LI , Jiadong LIU , Fei WANG , Wensi LIU , Jing YANG , Ying ZHANG . Preparation and catalytic performance of magnetic nano iron oxide by oxidation co-precipitation method. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1577-1582. doi: 10.11862/CJIC.20230469
16. [16]
  Guangming YIN , Huaiyao WANG , Jianhua ZHENG , Xinyue DONG , Jian LI , Yi'nan SUN , Yiming GAO , Bingbing WANG . Preparation and photocatalytic degradation performance of Ag/protonated g-C₃N₄ nanorod materials. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1491-1500. doi: 10.11862/CJIC.20240086
17. [17]
  Qi Li , Pingan Li , Zetong Liu , Jiahui Zhang , Hao Zhang , Weilai Yu , Xianluo Hu . Fabricating Micro/Nanostructured Separators and Electrode Materials by Coaxial Electrospinning for Lithium-Ion Batteries: From Fundamentals to Applications. Acta Physico-Chimica Sinica, 2024, 40(10): 2311030-. doi: 10.3866/PKU.WHXB202311030
18. [18]
  Di WU , Ruimeng SHI , Zhaoyang WANG , Yuehua SHI , Fan YANG , Leyong ZENG . Construction of pH/photothermal dual-responsive delivery nanosystem for combination therapy of drug-resistant bladder cancer cell. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1679-1688. doi: 10.11862/CJIC.20240135
19. [19]
  Chunmei GUO , Weihan YIN , Jingyi SHI , Jianhang ZHAO , Ying CHEN , Quli FAN . Facile construction and peroxidase-like activity of single-atom platinum nanozyme. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1633-1639. doi: 10.11862/CJIC.20240162
20. [20]
  Qingtang ZHANG , Xiaoyu WU , Zheng WANG , Xiaomei WANG . Performance of nano Li₂FeSiO₄/C cathode material co-doped by potassium and chlorine ions. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1689-1696. doi: 10.11862/CJIC.20240115

Get Citation

PDF

XML

Metrics

PDF Downloads(7)
Abstract views(608)
HTML views(137)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142