Citation: Lu Jinghe, Tan Shuzhen, Zhu Yuqing, Li Wei, Chen Tianxiao, Wang Yao, Liu Chen. Fluorescent Aptamer-functionalized Graphene Oxide Biosensor for Rapid Detection of Chloramphenicol[J]. Acta Chimica Sinica, ;2019, 77(3): 253-256. doi: 10.6023/A18100433 shu

Fluorescent Aptamer-functionalized Graphene Oxide Biosensor for Rapid Detection of Chloramphenicol

School of Chemical and Biological Engineering, Changsha University of Science and Technology, Changsha 410114

Corresponding author: Tan Shuzhen, tsz519@163.com
Received Date: 17 October 2018
Available Online: 17 March 2019
Fund Project: Project supported by the Scientific Research Fund of Hunan Provincial Education Department (No. 17C0033)the Scientific Research Fund of Hunan Provincial Education Department 17C0033

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A label free and rapid fluorescent method for quantitative detection of chloramphenicol (CAP) based on graphene oxide (GO) fluorescence functional G-quadruplex probe (FGP) was developed. The FGP consisted of a choramphenicol aptamer and a G-rich sequence. The aptamer was used to bind CAP and the G-quadruplex formed by G-rich sequence was employed as a signal reporter after binding to Thioflavin T (ThT). In the absence of CAP, the FGP was absorbed onto the surface of GO through π-π stacking interactions, which restrained the G-rich sequence to form a G-quadruplex structure. Thus, the fluorescent intensity of background was low. In the addition of the CAP, the aptamer part of FGP could recognize and bind CAP to form a target-FGP complex, which led to the desorption of the complex from GO. Therefore, the free G-rich sequence could form G-quadruplex structure and bind to ThT, resulting a increase in the fluorescence intensity of the solution. We observed that the fluorescence increasement of the sensing platform had a linear relationship with the concentrations of CAP in the range of 2~20 nmol/L, and the limit of detection was 1.45 nmol/L. Besides, this detection system was applied for detecting CAP in the spiked milk, the recovery rate was between 93.2%~103.3%. These results indicated that this developed method can be used to efficiently recognize CAP in real samples.
- aptamer,
- fluorescence,
- chloramphenicol,
- G-quadruplex,
- graphene oxide
1. [1]
  Wang, L.; Zhang, Y.; Gao, X.; Duan, Z.; Wang, S. J. Agric. Food Chem. 2010, 58, 3265. doi: 10.1021/jf903940h
2. [2]
  Wang, H.; Zhou, X.; Liu, Y.; Yang, H.; Guo, Q. J. Agric. Food Chem. 2011, 59, 3532. doi: 10.1021/jf2006062
3. [3]
  Webb, S.; Ternes, T.; Gibert, M.; Olejniczak, K. Toxicol. Lett. 2003, 142, 157. doi: 10.1016/S0378-4274(03)00071-7
4. [4]
  Duan, Y.; Wang, L.; Gao, Z. Talanta 2017, 165, 671. doi: 10.1016/j.talanta.2016.12.090
5. [5]
  Tan, Z.; Xu, H.; Li, G. Spectrochim. Acta A 2015, 149, 615. doi: 10.1016/j.saa.2015.04.109
6. [6]
  Pfenning, A. P.; Roybal, J. E.; Rupp, H. S.; Turnipseed, S. B.; Gonzales, S. A.; Hurlbut, J. A. J. AOAC Int. 2000, 83, 26.
7. [7]
  Wang, L.; Hai, Y.; Zhang, C. Anal. Chim. Acta 2008, 619, 54. doi: 10.1016/j.aca.2008.01.026
8. [8]
  Kawalski, D.; Poboży, E.; Trojanowicz, M. J. Autom. Methods Manag. Chem. 2011, 8565, 143416.
9. [9]
  Ramos, M.; Muñoz, P.; Aranda, A.; Rodriquez, I.; Diaz, R.; Blanca, J. J. Chromatogr. B 2003, 791, 31. doi: 10.1016/S1570-0232(03)00186-7
10. [10]
  Tian, H. Chemosphere 2011, 83, 349. doi: 10.1016/j.chemosphere.2010.12.016
11. [11]
  Kolosova, A. Y.; Samsonova, J. V.; Egorova, M. Food Agr. Immunol. 2000, 12, 115. doi: 10.1080/095401000404067
12. [12]
  Byzova, N. A.; Zvereva, E. A.; Zherdev, A. V.; Eremin, S. A.; Dzantiev, B. B. Talanta 2010, 81, 843. doi: 10.1016/j.talanta.2010.01.025
13. [13]
  He, J. L.; Zhu, S. L.; Wu, P.; Li, P. P.; Li, T.; Cao, Z. Biosens. Bioelectron. 2014, 60, 112. doi: 10.1016/j.bios.2014.03.065
14. [14]
  Chen, M.; Gan, N.; Zhang, H. Microchim. Acta 2016, 183, 1099. doi: 10.1007/s00604-015-1695-1
15. [15]
  Gao, H.; Pan, D.; Gan, N. Microchim. Acta 2015, 182, 2551. doi: 10.1007/s00604-015-1632-3
16. [16]
  Miao, Y. B.; Ren, H. X.; Gan, N. Anal. Chim. Acta 2016, 929, 49. doi: 10.1016/j.aca.2016.04.060
17. [17]
  Miao, Y. B.; Gan, N.; Li, T. H.; Zhang, H. R.; Cao, Y. T.; Jiang, Q. L. Sensor. Actuat. B-Chem. 2015, 220, 679. doi: 10.1016/j.snb.2015.05.106
18. [18]
  Sharma, R.; Ragavan, K. V.; Raghavarao, K. S. M. S. Biotechnology and Biochemical Engineering, Springer, Sin-gapore, 2016.
19. [19]
  Zhao, K.; Hao, Y.; Zhu, M.; Cheng, G. Acta Chim. Sinica 2018, 76, 168(in Chinese).
20. [20]
  Sun, H.; He, Q.; Yin, S.; Xu, K. Chin. J. Chem. 2017, 35, 1627. doi: 10.1002/cjoc.v35.10
21. [21]
  Yang, X.; Cai, H.; Bao, M.; Yu, J.; Lu, J.; Li, Y. Chin. J. Chem. 2017, 35, 1549. doi: 10.1002/cjoc.v35.10
22. [22]
  Chen, X. J.; Wang, Y. Z.; Zhang, Y. Y.; Chen, Z. H.; Liu, Y.; Li, Z. L.; Li, J. H. Anal. Chem. 2014, 86, 4278. doi: 10.1021/ac404070m
23. [23]
  Liu, X.; Wang, F.; Aizen, R. J. Am. Chem. Soc. 2013, 135, 11832. doi: 10.1021/ja403485r
24. [24]
  Yi, M.; Yang, S.; Peng, Z. Y.; Liu, C. H.; Li, J. S.; Zhong, W. W.; Yang, R. H.; Tan, W. H. Anal. Chem. 2014, 86, 3548. doi: 10.1021/ac5000015
25. [25]
  Zhang, P.; Gong, J. L.; Zeng, G. M.; Deng, C. H.; Yang, H. C.; Liu, H. Y.; Huan, S. Y. Chem. Eng. J. 2017, 322, 657. doi: 10.1016/j.cej.2017.04.068
26. [26]
  Luu, K. N.; Phan, A. T.; Kuryavyi, V. J. Am. Chem. Soc. 2006, 128, 9963. doi: 10.1021/ja062791w
27. [27]
  Guo, Y.; Sun, Y.; Shen, X.; Chen, X.; Yao, Y. R.; Xie, Y. F.; Hu, J. M.; Pei, R. J. Anal. Methods 2015, 7, 9615 doi: 10.1039/C5AY01840D
28. [28]
  Vummidi, B. R.; Alzeer, J.; Luedtke, N. W. ChemBioChem 2013, 14, 540. doi: 10.1002/cbic.v14.5
29. [29]
  Zhu, L. N.; Wu, B.; Kong, D. M. Nucleic Acids Res. 2013, 41, 4324. doi: 10.1093/nar/gkt103
30. [30]
  Huang, X. X.; Zhu, L. N.; Wu, B. Nucleic Acids Res. 2014, 42, 8719. doi: 10.1093/nar/gku526
31. [31]
  Zhang, Q.; Liu, Y. C.; Kong, D. M. Chem. Eur. J. 2015, 21, 13253. doi: 10.1002/chem.v21.38
32. [32]
  Jiang, H. X.; Liang, Z. Z.; Ma, Y. H. Anal. Chim. Acta 2016, 943, 114. doi: 10.1016/j.aca.2016.09.019
33. [33]
  Mohanty, J.; Barooah, N.; Dhamodharan, V. J. Am. Chem. Soc. 2012, 135, 367.
34. [34]
  Zhang, Q. J.; Peng, T.; Chen, D. D. J. Aoac. Int. 2013, 96, 897. doi: 10.5740/jaoacint.12-277
35. [35]
  Miao, Y. B.; Ren, H. X.; Gan, N.; Cao, Y. T.; Li, T. H.; Chen, Y. J. Biosens. Bioelectron. 2016, 81, 454. doi: 10.1016/j.bios.2016.03.034
36. [36]
  Fei, X.; Zhao, F.; Li, J. Anal. Chim. Acta 2007, 596, 79. doi: 10.1016/j.aca.2007.05.053
37. [37]
  Gao, H.; Gan, N.; Pan, D. Anal. Methods 2015, 7, 6528. doi: 10.1039/C5AY01379H
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