Citation: Duo Huanhuan, Liu Yanling, Wang Yawen, Tang Yun, Huang Weihua. Photocatalytically Renewable Electrode for On-Line Regeneration under Visible Light Irradiation and Real-Time Monitoring of Living Cells[J]. Acta Chimica Sinica, ;2017, 75(11): 1091-1096. doi: 10.6023/A17070330 shu

Photocatalytically Renewable Electrode for On-Line Regeneration under Visible Light Irradiation and Real-Time Monitoring of Living Cells

Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072

Corresponding author: Huang Weihua, whhuang@whu.edu.cn
Received Date: 22 July 2017
Available Online: 25 November 2017
Fund Project: the National Natural Science Foundation of China 21675121the National Natural Science Foundation of China 21375099Project supported by the National Natural Science Foundation of China (Nos. 21375099, 21675121)

Figures(5)

Electrode fouling and passivation is an inevitable problem which seriously affects the electrode performance in cell culture and detection. Construction of photocatalytically renewable electrode by combination of nanophotocatalysts with electrochemical sensing materials could provide a promising approach for highly efficient renewal of electrode surface without damaging its micro-or nanostructures. However, the reactive oxygen species generated during photocatalysis always cause damages to cells being adhered or cultured on the electrode surface, which precludes on-line renewal of electrode during cell culture and detection. To address this issue, based on the visible light-induced renewable electrode (poly(3, 4-ethylenedioxythiophene) (PEDOT)-modified TiO₂/CdS nanocomposites electrode we previously developed, a thin layer of gelatin hydrogel was spin-coated on the electrode in this work to realize efficient electrode renewal under visible light irradiation during the culture and detection of living cells. The optimized thickness (ca. 2 μm) of gelatin hydrogel was obtained by spin-coating under 3000 r/min. Benefitting from the network structure of gelatin hydrogel and the renewable performance of PEDOT@CdS/TiO₂ nanocomposites, the gelatin coating efficiently blocked the diffusion of biomacromolecules from the bulk medium to the electrode surface and thus significantly diminished the fouling caused by these macromolecules, while the pollutants derived from small molecules could be efficiently degraded under visible light irradiation. Meanwhile, gelatin coating did not induce obviously decline in detection sensitivity, and a low detection limit of 4.2 nmol/L (S/N=3) could be obtained towards electrochemical detection of nitric oxide (NO). Most importantly, the gelatin layer efficiently blocked the ultrashort-lived but highly reactive oxygen species such as OH·(generated by photocatalytic process) diffusing from the electrode surface to the cells, and the damages to the cells caused by these highly reactive species could be therefore significantly decreased. The results from live/dead cell staining demonstrated that almost all the cells (>95%) cultured on gelatin-coated electrodes maintain their viability when the electrode was irradiated by visible light for 6 h, while a considerable part of cells (>40%) culture on the uncoated electrode lost their viability under the same conditions. These features allowed on-line renewal of the electrode during cell culture and detection as well as real-time monitoring of NO released from the human umbilical vein endothelial cells (HUVECs).
- nanophotocatalysts,
- photocatalytically renewable electrode,
- visible light,
- gelatin,
- human umbilical vein endothelical cells,
- nitric oxide,
- real-time monitoring
1. [1]
  Lin, M. H.; Song, P.; Zhou, G. B.; Zuo, X. L.; Aldalbahi, A.; Lou, X. D.; Shi, J.; Fan, C. Nat. Protoc. 2016, 11, 1244. doi: 10.1038/nprot.2016.071
2. [2]
  Yan, X. Y.; Gu, Y.; Li, C.; Liu, T.; Zheng, B.; Li, Y.; Zhang, Z. Q.; Yang, M. Biosens. Bioelectron. 2016, 77, 1032. doi: 10.1016/j.bios.2015.10.085
3. [3]
  Bai, R. G.; Muthoosamy, K.; Zhou, M. F.; Ashokkumar, M.; Huang, N. M.; Manickam, S. Biosens. Bioelectron. 2017, 87, 622. doi: 10.1016/j.bios.2016.09.003
4. [4]
  Tang, C. K.; Vaze, A.; Shen, M.; Rusling, J. F. ACS Sens. 2016, 1, 1036. doi: 10.1021/acssensors.6b00256
5. [5]
  Yang, Z. Y.; Ma, W.; Ying, Y. L.; Long, Y. T. Acta Chim. Sinica 2017, 75, 671(in Chinese).
6. [6]
  Zhou, W. D.; Mahshid, S. S.; Wang, W. J.; Vallee-Belisle, A.; Zandstra, P. W.; Sargent, E. H.; Kelley, S. O. ACS Sens. 2017, 2, 495. doi: 10.1021/acssensors.7b00136
7. [7]
  Lin, X. Y.; Zhang, B. W.; Yang, Q.; Yan, F.; Hua, X.; Su, B. Anal. Chem. 2016, 88, 7821. doi: 10.1021/acs.analchem.6b01866
8. [8]
  Hui, N.; Sun, X. T.; Niu, S. Y.; Luo, X. L. ACS Appl. Mater. Interfaces 2017, 9, 2914. doi: 10.1021/acsami.6b11682
9. [9]
  Lee, J.; Arrigan, D. W. M.; Silvester, D. S. Anal. Chem. 2016, 88, 5104. doi: 10.1021/acs.analchem.5b04782
10. [10]
  Bhalla, V.; Carrara, S.; Stagni, C.; Samorì, B. Thin Solid Films 2010, 518, 3360. doi: 10.1016/j.tsf.2009.10.022
11. [11]
  Duan, W.; Ronen, A.; Walker, S.; Jassby, D. ACS Appl. Mater. Interfaces 2016, 8, 22574. doi: 10.1021/acsami.6b07196
12. [12]
  Hu, L. S.; Huo, K. F.; Chen, R. S.; Gao, B.; Fu, J. J.; Chu, P. K. Anal. Chem. 2011, 83, 8138. doi: 10.1021/ac201639m
13. [13]
  Guo, C. Y.; Huo, H. H.; Han, X.; Xu, C. L.; Li, H. L. Anal. Chem. 2014, 86, 876. doi: 10.1021/ac4034467
14. [14]
  Xu, J. Q.; Liu, Y. L.; Wang, Q.; Duo, H. H.; Zhang, X. W.; Li, Y. T.; Huan, W. H. Angew. Chem., Int. Ed. 2015, 54, 14402. doi: 10.1002/anie.201507354
15. [15]
  Duo, H. H.; Xu, J. Q.; Liu, Y. L.; Jin, Z. H.; Hu, X. B.; Huang, W. H. J. Electroanal. Chem. 2016, 781, 371. doi: 10.1016/j.jelechem.2016.06.046
16. [16]
  Xu, J. Q.; Duo, H. H.; Zhang, Y. G.; Zhang, X. W.; Fang, W.; Liu, Y. L.; Shen, A. G.; Hu, J. M.; Huang, W. H. Anal. Chem. 2016, 88, 3789. doi: 10.1021/acs.analchem.5b04810
17. [17]
  Atsumi, T.; Murata, J.; Kamiyanagi, I.; Fujisawa, S.; Ueha, T. Arch. Oral. Biol. 1998, 43, 73. doi: 10.1016/S0003-9969(97)00073-3
18. [18]
  Ferancová, A.; Rengaraj, S.; Kim, Y.; Labuda, J.; Sillanpää, M. Biosens. Bioelectron. 2010, 26, 314. doi: 10.1016/j.bios.2010.08.026
19. [19]
  Xu, F.; Zha, Y. P.; Wang, G. X.; Wang, Y.; Li, J. L. Acta Chim. Sinica 2009, 67, 957(in Chinese). doi: 10.3321/j.issn:0567-7351.2009.09.012
20. [20]
  Chen, A.; Zhou, M. Free Radical and Aging (Second Edition), People's Medical Publishing House, Beijing, 2011(in Chinese).
21. [21]
  Zhu, A. W.; Liu, Y.; Rui, Q.; Tian, Y. Chem. Commun. 2011, 47, 4279. doi: 10.1039/c0cc05821a
22. [22]
  Zhuang, M.; Ding, C. Q.; Zhu, A. W.; Tian, Y. Anal. Chem. 2016, 86, 1829.
23. [23]
  Li, L.; Zhu, A.; Tian, Y. Chem. Commun. 2013, 49, 1279. doi: 10.1039/c2cc38339j
24. [24]
  Liu, Y.; Chan-Park, M. B. Biomaterials 2010, 83, 1158.
25. [25]
  Caliari, S. R.; Burdick, J. A. Nat. Methods 2016, 13, 405. doi: 10.1038/nmeth.3839
26. [26]
  Yu, P.; Zhou, H.; Cheng, H.; Qian, Q.; Mao, L. Q. Anal. Chem. 2011, 83, 5715. doi: 10.1021/ac200942a
27. [27]
  Privett, B. J.; Shin, J. H.; Schoenfisch, M. H. Chem. Soc. Rev. 2010, 39, 1925. doi: 10.1039/b701906h
28. [28]
  Coneski, P. N.; Schoenfisch, M. H. Chem. Soc. Rev. 2012, 41, 3753. doi: 10.1039/c2cs15271a
1. [1]
  Bing LIU , Huang ZHANG , Hongliang HAN , Changwen HU , Yinglei ZHANG . Visible light degradation of methylene blue from water by triangle Au@TiO₂ mesoporous catalyst. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 941-952. doi: 10.11862/CJIC.20230398
2. [2]
  Wei Huang , Weiwei Chen , Yongxing Tang . Green Mountains and Blue Waters Spanning Nine Centuries: Decrypting “The Picture of a Thousand Miles of Rivers and Mountains” from a Chemical Perspective. University Chemistry, 2024, 39(9): 189-195. doi: 10.12461/PKU.DXHX202312075
3. [3]
  Zhen Yao , Bing Lin , Youping Tian , Tao Li , Wenhui Zhang , Xiongwei Liu , Wude Yang . Visible-Light-Mediated One-Pot Synthesis of Secondary Amines and Mechanistic Exploration. University Chemistry, 2024, 39(5): 201-208. doi: 10.3866/PKU.DXHX202311033
4. [4]
  Bo YANG , Gongxuan LÜ , Jiantai MA . Nickel phosphide modified phosphorus doped gallium oxide for visible light photocatalytic water splitting to hydrogen. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 736-750. doi: 10.11862/CJIC.20230346
5. [5]
  Yurong Tang , Yunren Shi , Yi Xu , Bo Qin , Yanqin Xu , Yunfei Cai . Innovative Experiment and Course Transformation Practice of Visible-Light-Mediated Photocatalytic Synthesis of Isoquinolinone. University Chemistry, 2024, 39(5): 296-306. doi: 10.3866/PKU.DXHX202311087
6. [6]
  Zhiquan Zhang , Baker Rhimi , Zheyang Liu , Min Zhou , Guowei Deng , Wei Wei , Liang Mao , Huaming Li , Zhifeng Jiang . Insights into the Development of Copper-based Photocatalysts for CO₂ Conversion. Acta Physico-Chimica Sinica, 2024, 40(12): 2406029-. doi: 10.3866/PKU.WHXB202406029
7. [7]
  Jie Li , Huida Qian , Deyang Pan , Wenjing Wang , Daliang Zhu , Zhongxue Fang . Efficient Synthesis of Anethaldehyde Induced by Visible Light. University Chemistry, 2024, 39(4): 343-350. doi: 10.3866/PKU.DXHX202310076
8. [8]
  Juntao Yan , Liang Wei . 2D S-Scheme Heterojunction Photocatalyst. Acta Physico-Chimica Sinica, 2024, 40(10): 2312024-. doi: 10.3866/PKU.WHXB202312024
9. [9]
  Yuanyin Cui , Jinfeng Zhang , Hailiang Chu , Lixian Sun , Kai Dai . Rational Design of Bismuth Based Photocatalysts for Solar Energy Conversion. Acta Physico-Chimica Sinica, 2024, 40(12): 2405016-. doi: 10.3866/PKU.WHXB202405016
10. [10]
  Hongbo Zhang , Yihong Tang , Suxia Zhang , Yuanting Li . Electrochemical Monitoring of Photocatalytic Degradation of Phenol Pollutants: A Recommended Comprehensive Analytical Chemistry Experiment. University Chemistry, 2024, 39(6): 326-333. doi: 10.3866/PKU.DXHX202310013
11. [11]
  Asif Hassan Raza , Shumail Farhan , Zhixian Yu , Yan Wu . 用于高效制氢的双S型ZnS/ZnO/CdS异质结构光催化剂. Acta Physico-Chimica Sinica, 2024, 40(11): 2406020-. doi: 10.3866/PKU.WHXB202406020
12. [12]
  Yi YANG , Shuang WANG , Wendan WANG , Limiao CHEN . Photocatalytic CO₂ reduction performance of Z-scheme Ag-Cu₂O/BiVO₄ photocatalyst. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 895-906. doi: 10.11862/CJIC.20230434
13. [13]
  Juan WANG , Zhongqiu WANG , Qin SHANG , Guohong WANG , Jinmao LI . NiS and Pt as dual co-catalysts for the enhanced photocatalytic H₂ production activity of BaTiO₃ nanofibers. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1719-1730. doi: 10.11862/CJIC.20240102
14. [14]
  Wei Zhong , Dan Zheng , Yuanxin Ou , Aiyun Meng , Yaorong Su . K原子掺杂高度面间结晶的g-C₃N₄光催化剂及其高效H₂O₂光合成. Acta Physico-Chimica Sinica, 2024, 40(11): 2406005-. doi: 10.3866/PKU.WHXB202406005
15. [15]
  Jingzhao Cheng , Shiyu Gao , Bei Cheng , Kai Yang , Wang Wang , Shaowen Cao . 4-氨基-1H-咪唑-5-甲腈修饰供体-受体型氮化碳光催化剂的构建及其高效光催化产氢研究. Acta Physico-Chimica Sinica, 2024, 40(11): 2406026-. doi: 10.3866/PKU.WHXB202406026
16. [16]
  Zhanggui DUAN , Yi PEI , Shanshan ZHENG , Zhaoyang WANG , Yongguang WANG , Junjie WANG , Yang HU , Chunxin LÜ , Wei ZHONG . Preparation of UiO-66-NH₂ supported copper catalyst and its catalytic activity on alcohol oxidation. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 496-506. doi: 10.11862/CJIC.20230317
17. [17]
  Meng Lin , Hanrui Chen , Congcong Xu . Preparation and Study of Photo-Enhanced Electrocatalytic Oxygen Evolution Performance of ZIF-67/Copper(I) Oxide Composite: A Recommended Comprehensive Physical Chemistry Experiment. University Chemistry, 2024, 39(4): 163-168. doi: 10.3866/PKU.DXHX202308117
18. [18]
  Qingqing SHEN , Xiangbowen DU , Kaicheng QIAN , Zhikang JIN , Zheng FANG , Tong WEI , Renhong LI . Self-supporting Cu/α-FeOOH/foam nickel composite catalyst for efficient hydrogen production by coupling methanol oxidation and water electrolysis. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1953-1964. doi: 10.11862/CJIC.20240028
19. [19]
  Kun WANG , Wenrui LIU , Peng JIANG , Yuhang SONG , Lihua CHEN , Zhao DENG . Hierarchical hollow structured BiOBr-Pt catalysts for photocatalytic CO₂ reduction. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1270-1278. doi: 10.11862/CJIC.20240037
20. [20]
  Zijian Jiang , Yuang Liu , Yijian Zong , Yong Fan , Wanchun Zhu , Yupeng Guo . Preparation of Nano Zinc Oxide by Microemulsion Method and Study on Its Photocatalytic Activity. University Chemistry, 2024, 39(5): 266-273. doi: 10.3866/PKU.DXHX202311101

Get Citation

PDF

XML

Metrics

PDF Downloads(7)
Abstract views(2779)
HTML views(136)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142