Citation: Wang Qin, Nie Zhou, Hu Yufang, Yao Shouzhuo. Electrochemical Assay for Acetylcholinesterase Activity Detection Based on Unique Electro-catalytic Activity of Cu(Ⅱ)-thiol Coordination Polymer[J]. Acta Chimica Sinica, ;2017, 75(11): 1109-1114. doi: 10.6023/A17070321 shu

Electrochemical Assay for Acetylcholinesterase Activity Detection Based on Unique Electro-catalytic Activity of Cu(Ⅱ)-thiol Coordination Polymer

Wang Qin ^a ,
Nie Zhou ^a,, ,
Hu Yufang ^a,b,, ,
Yao Shouzhuo ^a

a.
College of Chemistry and Chemical Engineering, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082
b.
Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211

Corresponding author: Nie Zhou, niezhou.hnu@gmail.com Hu Yufang, huyufang@nbu.edu.cn
Received Date: 18 July 2017
Available Online: 8 November 2017
Fund Project: the National Natural Science Foundation of China 21235002Project supported by the National Natural Science Foundation of China (Nos. 21575038, 21235002, 21605089, and 21305037), the Foundation for Innovative Research Groups of NSFC (No. 21521063), and the Natural Science Foundation of Hunan Province (No. 2015JJ1005)Foundation for Innovative Research Groups of NSFC 21521063the National Natural Science Foundation of China 21605089the National Natural Science Foundation of China 21575038the Natural Science Foundation of Hunan Province 2015JJ1005the National Natural Science Foundation of China 21305037

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Acetylcholinesterase (AChE), as a key enzyme, ubiquitously exists in the peripheral nervous system. It mainly functions terminating neurotransmission at the cholinergic synapse through the rapid hydrolysis of acetylcholine (a neurotransmitter) into choline and acetate, which is intimately related to Alzheimer's disease, inflammatory processes, and nerve-agent poisoning. In this study, we developed a novel electrochemical method for probing AChE activity and screening its potential inhibitor based on the in-situ formation of thiocholine-Cu(Ⅱ) coordination polymer[denoted as TCh-Cu(Ⅱ) CP]. The detection mechanism is on the basis of the concept, that is, AChE could catalyze the hydrolysis of its substrate acetylthiocholine (ATCh) to produce a thiol-compound thiocholine (TCh). Subsequently, TCh reacted with Cu(Ⅱ) to form a positively charged TCh-Cu(Ⅱ) CP via S-Cu bond. Since the graphene (GO) is a negative compound due to its plenty of carboxyl groups, TCh-Cu(Ⅱ) CP could be adsorbed onto the graphene-modified glassy carbon electrode (GO/GCE) via electrostatic interaction. What's more, the CP/GO/GCE can electro-catalyze the O-phenylenediamine (OPD), generating a high current signal. We also conducted a series of experiments to verify the formation and electro-catalysis of TCh-Cu(Ⅱ) CP and investigated the selectivity of the TCh-Cu(Ⅱ) CP-based assay for AChE. As a result, it is clearly observed that the electrochemical response gradually increases with the increasing concentrations of AChE (0.05~100 mU·mL^-1) and a detection limit of our method is estimated to be ca. 0.03 mU·mL^-1 (S/N=3). Furthermore, compared to the traditional methods, our electrochemical assay has more simple detection procedure with better sensitivity and selectivity and has great potential in applications in many important areas, such as neurobiology, toxicology, and pharmacology, especially for the effective treatment of Alzheimer's disease.
- electrochemical biosensor,
- TCh-Cu (Ⅱ) coordination polymer,
- acetylcholinesterase,
- inhibitor screening
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