Capillary Electrophoresis Immunoassay by Gold Nanoparticles Assisted Signal Generation and Sequential Stacking
- Corresponding author: Zhang Zhaoxiang, qustzhzhx@126.com
Citation: Zhang Zhaoxiang, Luan Wenxiu, Zhang Chaoying, Liu Yujie. Capillary Electrophoresis Immunoassay by Gold Nanoparticles Assisted Signal Generation and Sequential Stacking[J]. Acta Chimica Sinica, ;2017, 75(4): 403-407. doi: 10.6023/A16110599
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a, CHRP=0, CAb=0; b, CHRP=5 mg/L, CAb=2 mg/L; c, CHRP=10 mg/L, CAb=4 mg/L; d, CHRP=20 mg/L, CAb=8 mg/L
Base injection time: 1, 90 s; 2, 120 s; 3, 150 s; 4, 180 s. The running buffer was 10.0 mmol/L BR+1.0 mmol/L H2O2+1.0% PVP, pH 4.0
The concentrations of HRP-Au-Ab probe were constant. Electropherograms of a, b, c and d correspond to 0, 2.0, 5.0, and 10.0 ng/mL BTX-B, respectively. The running buffer was 10.0 mmol/L BR+1.0 mmol/L H2O2+1.0% PVP, pH 4.0; OAP concentration: 1.0 mmol/L; Sample injection, 10 kV for 330 s; Separation voltage, 15 kV; EC detection potential, -0.35 V