Citation: Ji Guang, Yan Lulin, Wang Hui, Ma Lian, Xu Bin, Tian Wenjing. Efficient Near-infrared AIE Nanoparticles for Cell Imaging[J]. Acta Chimica Sinica, ;2016, 74(11): 917-922. doi: 10.6023/A16080430 shu

Efficient Near-infrared AIE Nanoparticles for Cell Imaging

1.
State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012

Corresponding author: Xu Bin, xubin@jlu.edu.cn Tian Wenjing, wjtian@jlu.edu.cn
Received Date: 24 August 2016

Fund Project: the Natural Science Foundation of China 21221063and Program for Chang Jiang Scholars and Innovative Research Team in University IRT101713018the Natural Science Foundation of China 51573068the Natural Science Foundation of China 51373063Project supported by 973 Program 2013CB834701

Figures(8)

Near-infrared fluorescence signals are highly desirable to acheieve high resolution in biological imaging. We encapsulated hydrophobic AIE (aggregation-induced emission) fluorophores into the biocompatible Pluronic F-127 NPs for cellular imaging and efficiently enhance the near-infrared AIE fluorophore emission. AIE molecule 2-(4-bromophenyl)-3-(4-(4-(diphenylamino) styryl) phenyl) fumaronitrile (TPABDFN) with near-infrared emission was synthesized and selected as the fluorescence resonance energy transfer (FRET) acceptor. (2-p-tolylethene-1, 1, 2-triyl) tribenzene (TPE-Me) was a blue-emitting AIE molecule, which spectrum was matching with TPABDFN. TPE-Me@F127 NPs emission was 480 nm, TPABDFN@F127 NPs maximum absorption wavelength was also 480 nm, that the absorption had a large area of overlapping with the TPE-Me@F127 NPs emission spectrum and leaded to efficient energy transfer, so TPE-Me was selected as the FRET donor. By encapsulating both TPE-Me donor and TPABDFN acceptor simultaneously within the NPs, a significant FRET effect was induced. FRET pairs of different ratios was co-encapsulated into the F127 NPs to optimize the fluorescence signals. The maximum of fluorescence quantum yield was 19.9%, energy transfer efficiency was 43.5%. TPABDFN@F127 NPs only had weak fluorescence, but the TPABDFN/TPE-Me@F127 NPs showed bright fluorescence signal. Fluorescence resonance energy transfer contributed to the notable increase of acceptor emission The fluorescence quantum yield had 10-fold enhancement of the TPABDFN. In addition, the obtained TPABDFN/TPE-Me@F127 NPs showed a large Stokes shift of 265 nm, which can be used to avoid the interference between excitation and emission light, as well as the near-infrared emission spectrum away from the organism auto-fluorescence, which was beneficial for the bio-application. Fluorescent probe emission in the far red/near-infrared (FR/NIR) (650~900 nm) region for biological detection also can greatly reduce the damage to living body. And TPABDFN/TPE-Me@F127 NPs had low cytotoxicity, good biocompatibility, stability and anti-photobleaching. The TPABDFN/TPE-Me@F127 NPs achieved good imaging result on HepG2 cell cytoplasm.
- aggregation-induced emission,
- energy transfer,
- self-assembly,
- nanoparticles,
- cell imaging
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沈阳化工大学材料科学与工程学院沈阳 110142