Citation: Cao Chan, Liao Dongfang, Ying Yilun, Long Yitao. Detection of Single Oligonucleotide by an Aerolysin Nanopore[J]. Acta Chimica Sinica, ;2016, 74(9): 734-737. doi: 10.6023/A16070352 shu

Detection of Single Oligonucleotide by an Aerolysin Nanopore

1.
Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237

Received Date: 19 July 2016

Fund Project: the Program of Introducing Talents of Discipline to Universities B16017the National Natural Science Foundation of China 21421004the National Natural Science Foundation of China 21327807

Figures(3)

Since the nanopore single-molecule technology has been proposed, it remains a big challenge to generate a sensitive and stable nano-scale pore. In order to achieve this goal, membrane proteins, solid-state nanopore and other materials such as DNA origami have been involved to fabricate a suitable nanopore. Compared to the solid-state nanopores, biological nanopores perform a higher resolution for single molecule analysis. Therefore, the investigation of finding new biological nanopores is very important to realize the discrimination of single oligonucleotide. Aerolysin biological nanopore has been applied for the study of oligosaccharides, peptides, protein unfolding and small organic molecules so far. Here, we report that Aerolysin could be utilized for oligonucleotide analysis. The data demonstrated that Aerolysin nanopore has a high resolution both for current and time compared with other most widely used wild-type biological nanopores, such as α-hemolysin and Mycobacterium smegmatis porin A (MspA). It may be because of its narrow diameter and positive charged amino acids in the lumen. One Aerolysin pore generates a 50 pA constant ion current in 1 mol/L KCl solution, as a three nucleotides length oligonucleotides (5'-AGG-3') traversing through nanopore could induce nearly 40% current blockage. In comparison, no current blockage signals were observed when 5'-AGG-3' driven from either cis or trans side of the α-hemolysin nanopore. Furthermore, the statistical analysis of duration time of single oligonucleotide through Aerolysin indicates a relationship scale with applied voltage, as the voltage increased from 80 to 160 mV, the duration gradually decreased. Although Aerolysin nanopore has been investigated for nearly 10 years, its ability to detect oligonucleotide was not highlighted. Our findings explored high sensing capabilities of Aerolysin nanopore in the analysis of single oligonucleotide, and extended its application to single-molecule nucleic acid analysis. Aerolysin is a promising candidate for the DNA sensing, DNA damage detection, microRNA analysis and other single molecule investigations.
- Aerolysin,
- biological nanopores,
- single-molecule detection,
- oligonucleotides
1. [1]
  Kasianowicz J. J., Brandin E., Branton D., Deamer D. W. Proc. Natl. Acad. Sci. U. S. A. 1996, 93(24), 13770. doi: 10.1073/pnas.93.24.13770
2. [2]
  Ying Y.-L., Zhang X., Liu Y., Xue M.-Z., Li H.-L., Long Y.-T. Acta Chim. Sinica, 2013, 71:44 (in Chinese). doi: 10.6023/A12110982
3. [3]
  Wang Y., Yu X.-F., Liu Y.-Y., Xie X., Cheng X.-L., Huang S.-M., Wang Z...-M. Acta Chim. Sinica, 2014, 72:378 (in Chinese). doi: 10.6023/A13121208
4. [4]
  Ying Y.-L., Cao C., Long Y.-T. Analyst, 2014, 139:3826. doi: 10.1039/C4AN00706A
5. [5]
  Branton D., Deamer D. W., Marziali A., Bayley H., Benner S. A., Butler T., Di Ventra M., Garaj S., Hibbs A., Huang X., Jovanovich S. B., Krstic P. S., Lindsay S., Ling X. S., Mastrangelo C. H., Meller A., Oliver J. S., Pershin Y. V., Ramsey J. M., Riehn R., Soni G. V., Tabard-Cossa V., Wanunu M., Wiggin M., Schloss J. A...Nat. Biotechnol., 2008, 26:1146. doi: 10.1038/nbt.1495
6. [6]
  Clarke J., Wu H.-C., Jayasinghe L., Patel A., Reid S., Bayley H...Nat. Nanotechnol., 2009, 4:265. doi: 10.1038/nnano.2009.12
7. [7]
  Kumar S., Tao C., Chien M., Hellner B., Balijepalli A., Robertson J. W., Li Z., Russo J. J., Reiner J. E., Kasianowicz J. J., Ju J...Sci. Rep., 2012, 2:684.
8. [8]
  Stoddart D., Heron A. J., Mikhailova E., Maglia G., Bayley H...Proc. Natl. Acad. Sci. U. S. A., 2009, 106:7702; (b) Manrao E. A., Derrington I. M., Pavlenok M., Niederweis M., Gundlach J. H...PLoS ONE 2011, 6, e25723.
9. [9]
  Wang H.-Y., Li Y., Qin L.-X., Heyman A., Shoseyov O., Willner I., Long Y.-T., Tian H...Chem. Commun., 2013, 49:1741; (b) Butler T. Z., Pavlenok M., Derrington I. M., Niederweis M., Gundlach J. H...Proc. Natl. Acad. Sci. U. S. A., 2008, 105:20647.
10. [10]
  Manrao E. A., Derrington I. M., Laszlo A. H., Langford K. W., Hopper M. K., Gillgren N., Pavlenok M., Niederweis M., Gundlach J. H...Nat. Biotechnol., 2012, 30:349. doi: 10.1038/nbt.2171
11. [11]
  Parker M. W., Buckley J. T., Postma J. P. M., Tucker A. D., Leonard K., Pattus F., Tsernoglou D. Nature, 1994, 367:292. doi: 10.1038/367292a0
12. [12]
  Stefureac R., Long Y.-T., Kraatz H.-B., Howard P., Lee J. S. Biochemistry, 2006, 45:9172.
13. [13]
  Pastoriza-Gallego M., Rabah L., Gibrat G., Thiebot B., van der Goot F. G., Auvray L., Betton J. M., Pelta J. J. J. Am. Chem. Soc., 2011, 133:2923. doi: 10.1021/ja1073245
14. [14]
  Baaken G., Halimeh I., Bacri L., Pelta J., Oukhaled A., Behrends J. C. ACS Nano, 2015, 9:6443. doi: 10.1021/acsnano.5b02096
15. [15]
  Fennouri A., Przybylski C., Pastoriza-Gallego M., Bacri L., Auvray L., Daniel R. ACS Nano, 2012, 6:9672. doi: 10.1021/nn3031047
16. [16]
  Fennouri A., Daniel R., Pastoriza-Gallego M., Auvray L., Pelta J., Bacri L...Anal. Chem., 2013, 85:8488; (b) Cressiot B., Braselmann E., Oukhaled A., Elcock A. H., Pelta J., Clark P. L...ACS Nano, 2015, 9:9050.
17. [17]
  Degiacomi M. T., Iacovache I., Pernot L., Chami M., Kudryashev M., Stahlberg H., van der Goot F. G., Peraro M. D...Nat. Chem. Biol., 2013, 9:623. doi: 10.1038/nchembio.1312
18. [18]
  Chan C., Ying Y.-L., Hu Z.-L., Liao D.-F., Tian H., Long Y...-T. Nat. Nanotechnol., 2016, 11:713. doi: 10.1038/nnano.2016.66
19. [19]
  Cao C., Ying Y.-L., Gu Z., Long Y...-T. Anal. Chem., 2014, 86:11946. doi: 10.1021/ac504233s
1. [1]
  Meijin Li , Xirong Fu , Xue Zheng , Yuhan Liu , Bao Li . The Marvel of NAD⁺: Nicotinamide Adenine Dinucleotide. University Chemistry, 2024, 39(9): 35-39. doi: 10.12461/PKU.DXHX202401027
2. [2]
  Chunmei GUO , Weihan YIN , Jingyi SHI , Jianhang ZHAO , Ying CHEN , Quli FAN . Facile construction and peroxidase-like activity of single-atom platinum nanozyme. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1633-1639. doi: 10.11862/CJIC.20240162
3. [3]
  Junjie Zhang , Yue Wang , Qiuhan Wu , Ruquan Shen , Han Liu , Xinhua Duan . Preparation and Selective Separation of Lightweight Magnetic Molecularly Imprinted Polymers for Trace Tetracycline Detection in Milk. University Chemistry, 2024, 39(5): 251-257. doi: 10.3866/PKU.DXHX202311084
4. [4]
  Pingwei Wu . Application of Diamond Software in Simplex Teaching. University Chemistry, 2024, 39(3): 118-121. doi: 10.3866/PKU.DXHX202311043
5. [5]
  Liwei Wang , Guangran Ma , Li Wang , Fugang Xu . A Comprehensive Analytical Chemistry Experiment: Colorimetric Detection of Vitamin C Using Nanozyme and Smartphone. University Chemistry, 2024, 39(8): 255-262. doi: 10.3866/PKU.DXHX202312094
6. [6]
  Zhengli Hu , Jia Wang , Yi-Lun Ying , Shaochuang Liu , Hui Ma , Wenwei Zhang , Jianrong Zhang , Yi-Tao Long . Exploration of Ideological and Political Elements in the Development History of Nanopore Electrochemistry. University Chemistry, 2024, 39(8): 344-350. doi: 10.3866/PKU.DXHX202401072
7. [7]
  Rui Li , Huan Liu , Yinan Jiao , Shengjian Qin , Jie Meng , Jiayu Song , Rongrong Yan , Hang Su , Hengbin Chen , Zixuan Shang , Jinjin Zhao . 卤化物钙钛矿的单双向离子迁移. Acta Physico-Chimica Sinica, 2024, 40(11): 2311011-. doi: 10.3866/PKU.WHXB202311011
8. [8]
  Xiaowei TANG , Shiquan XIAO , Jingwen SUN , Yu ZHU , Xiaoting CHEN , Haiyan ZHANG . A zinc complex for the detection of anthrax biomarker. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1850-1860. doi: 10.11862/CJIC.20240173
9. [9]
  Fei Xie , Chengcheng Yuan , Haiyan Tan , Alireza Z. Moshfegh , Bicheng Zhu , Jiaguo Yu . d带中心调控过渡金属单原子负载COF吸附O₂的理论计算研究. Acta Physico-Chimica Sinica, 2024, 40(11): 2407013-. doi: 10.3866/PKU.WHXB202407013
10. [10]
  Yang YANG , Pengcheng LI , Zhan SHU , Nengrong TU , Zonghua WANG . Plasmon-enhanced upconversion luminescence and application of molecular detection. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 877-884. doi: 10.11862/CJIC.20230440
11. [11]
  Hong LI , Xiaoying DING , Cihang LIU , Jinghan ZHANG , Yanying RAO . Detection of iron and copper ions based on gold nanorod etching colorimetry. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 953-962. doi: 10.11862/CJIC.20230370
12. [12]
  Yuhao SUN , Qingzhe DONG , Lei ZHAO , Xiaodan JIANG , Hailing GUO , Xianglong MENG , Yongmei GUO . Synthesis and antibacterial properties of silver-loaded sod-based zeolite. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 761-770. doi: 10.11862/CJIC.20230169
13. [13]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403
14. [14]
  Yufang GAO , Nan HOU , Yaning LIANG , Ning LI , Yanting ZHANG , Zelong LI , Xiaofeng LI . Nano-thin layer MCM-22 zeolite: Synthesis and catalytic properties of trimethylbenzene isomerization reaction. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1079-1087. doi: 10.11862/CJIC.20240036
15. [15]
  Feng Zheng , Ruxun Yuan , Xiaogang Wang . “Research-Oriented” Comprehensive Experimental Design in Polymer Chemistry: the Case of Polyimide Aerogels. University Chemistry, 2024, 39(10): 210-218. doi: 10.12461/PKU.DXHX202404027
16. [16]
  Xingyang LI , Tianju LIU , Yang GAO , Dandan ZHANG , Yong ZHOU , Meng PAN . A superior methanol-to-propylene catalyst: Construction via synergistic regulation of pore structure and acidic property of high-silica ZSM-5 zeolite. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1279-1289. doi: 10.11862/CJIC.20240026
17. [17]
  Di Yang , Jiayi Wei , Hong Zhai , Xin Wang , Taiming Sun , Haole Song , Haiyan Wang . Rapid Detection of SARS-CoV-2 Using an Innovative “Magic Strip”. University Chemistry, 2024, 39(4): 373-381. doi: 10.3866/PKU.DXHX202312023
18. [18]
  Haitang WANG , Yanni LING , Xiaqing MA , Yuxin CHEN , Rui ZHANG , Keyi WANG , Ying ZHANG , Wenmin WANG . Construction, crystal structures, and biological activities of two Ln^Ⅲ₃ complexes. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1474-1482. doi: 10.11862/CJIC.20240188
19. [19]
  Jianfeng Yan , Yating Xiao , Xin Zuo , Caixia Lin , Yaofeng Yuan . Comprehensive Chemistry Experimental Design of Ferrocenylphenyl Derivatives. University Chemistry, 2024, 39(4): 329-337. doi: 10.3866/PKU.DXHX202310005
20. [20]
  Zhibei Qu , Changxin Wang , Lei Li , Jiaze Li , Jun Zhang . Organoid-on-a-Chip for Drug Screening and the Inherent Biochemistry Principles. University Chemistry, 2024, 39(7): 278-286. doi: 10.3866/PKU.DXHX202311039

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(1357)
HTML views(148)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142