Citation: Huang Wenguang, Sun Hongfei, Zhang Shujuan. Facile Synthesis and Evaluation of Size-tunable Immobilized Laccase-mediator Microreactor[J]. Acta Chimica Sinica, ;2016, 74(6): 518-522. doi: 10.6023/A16030158 shu

Facile Synthesis and Evaluation of Size-tunable Immobilized Laccase-mediator Microreactor

1.
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023

Received Date: 30 March 2016

Fund Project: the National Natural Science Foundation of China 21522702the National Natural Science Foundation of China 51378254

Figures(5)

A series of immobilized laccase-mediator microreactor (LMMR) was prepared in a one-pot process by simultaneously immobilizing Trametes versicolor laccase and acetylacetone (AA) into size-tunable chitosan copper-polyacrylamide hydrogel beads. The polymerization was induced by a laccase-AA-persulfate ternary initiating system and was finished within the chitosan beads at room temperature. The preparation conditions for the LMMR were optimized by an orthogonal array design. The method developed in this work, for the first time, realized the co-immobilization of laccase and mediator in microreactors of tunable size and mechanic strength. Experimental results from scanning electron microscopy and nitrogen adsorption-desorption analysis indicate that the resulting LMMR had a core-shell structure. Chitosan copper served as the mechanical shell, whereas polyacrylamide hydrogel was the core of three-dimensional network. Throughout the hydrogel beads, there were abundant mesopores of size in the range of 2~8 nm. The microreactor beads could endure a 20 N pressure in the axial direction, ensuring the structural integrity in the practical application in wastewater. The loading efficiency of laccase in the microreactor reached up to 93.5%. As compared with the free laccase, the LMMR showed better storage stability and higher tolerance to changes in solution pH and temperature. In the enzymatic conversion of malachite green (MG), benefited from the mediation effect of the immobilized AA, the LMMR still worked after 17 cycling runs (12 h for each cycle), which was 3-fold longer than that of a free laccase-mediator system. The successful recovery of both laccase and mediator is promising to reduce the cost for the application of laccase in wastewater treatment and might be helpful to cut down the secondary pollution from free laccase mediators. These results demonstrate that this novel one-pot synthesis was a useful strategy in the immobilization of laccase. The LMMR has a great potential in large-scale application for dyeing effluent treatment.
- laccase,
- mediator,
- acetylacetone,
- microreactor,
- malachite green
1. [1]
  Rahman, I. A.; Saad, B.; Shaidan, S.; Rizal, E. S. S. Bioresour. Technol. 2005, 96, 1578. doi: 10.1016/j.biortech.2004.12.015
2. [2]
  Berberidou, C.; Poulios, I.; Xekoukoulotakis, N. P.; Mantzavinos, D. Appl. Catal., B 2007, 74, 63. doi: 10.1016/j.apcatb.2007.01.013
3. [3]
  Chen, C. C.; Lu, C. S.; Chung, Y. C.; Jan, J. L. J. Hazard. Mater. 2007, 141, 520. doi: 10.1016/j.jhazmat.2006.07.011
4. [4]
  Song, X. J.; Wu, B. D.; Zhang, S. J. Acta Chim. Sinica 2014, 72, 461. doi: 10.6023/A14010027
5. [5]
  Pandey, A.; Singh, P.; Iyengar, L. Int. Biodeterior. Biodegrad. 2007, 59, 73. doi: 10.1016/j.ibiod.2006.08.006
6. [6]
  Michniewicz, A.; Ledakowicz, S.; Ullrich, R.; Hofrichter, M. Dyes Pigm. 2008, 77, 295. doi: 10.1016/j.dyepig.2007.05.015
7. [7]
  Huang, J.; Zhou, J. Y.; Xiao, H. Y.; Long, S. Y.; Wang, J. T. Acta Chim. Sinica 2005, 63, 1343.
8. [8]
  Durán, N.; Rosa, M. A.; D'Annibale, A.; Gianfreda, L. Enzyme Microb. Technol. 2002, 31, 907. doi: 10.1016/S0141-0229(02)00214-4
9. [9]
  Cetinus, S. A.; Sahin, E.; Saraydin, D. Food Chem. 2009, 114, 962. doi: 10.1016/j.foodchem.2008.10.049
10. [10]
  Rosevear, A.; Kent, C. A.; Thomson, A. R.; Bucke, C. In Enzyme Engineering, Vol. 4, Eds.: Broun, G.; Manecke, G.; Wingard, L., Jr., Springer, US, 1978, p. 415.
11. [11]
  Tanaka, H.; Kurosawa, H.; Kokufuta, E.; Veliky, I. A. Biotechnol. Bioeng. 1984, 26, 1393. doi: 10.1002/(ISSN)1097-0290
12. [12]
  de Alteriis, E.; Parascandola, P.; Pecorella, M.; Scardi, V. Biotechnol. Tech. 1988, 2, 205. doi: 10.1007/BF01875766
13. [13]
  Dealteriis, E.; Parascandola, P.; Salvadore, S.; Scardi, V. J. Chem. Technol. Biotechnol. 1985, 35, 60. doi: 10.1002/(ISSN)1097-4660
14. [14]
  Zouari-Mechichi, H.; Mechichi, T.; Dhouib, A.; Sayadi, S.; Martínez, A. T.; Martínez, M. J. Enzyme Microb. Technol. 2006, 39, 141. doi: 10.1016/j.enzmictec.2005.11.027
15. [15]
  Yang, H.; Sun, H. F.; Zhang, S. J.; Wu, B. D.; Pan, B. C. Environ. Sci. Pollut. Res. 2015, 22, 10882. doi: 10.1007/s11356-015-4312-2
16. [16]
  Sun, H. F.; Huang, W. G.; Yang, H.; Zhang, S. J. J. Colloid Interface Sci. 2016, 471, 20. doi: 10.1016/j.jcis.2016.03.009
17. [17]
  Daâssi, D.; Rodríguez-Couto, S.; Nasri, M.; Mechichi, T. Int. Biodeterior. Biodegrad. 2014, 90, 71.
18. [18]
  Sun, H.; Yang, H.; Huang, W.; Zhang, S. J. Colloid Interface Sci. 2015, 450, 353. doi: 10.1016/j.jcis.2015.03.037
19. [19]
  Ikeda, R.; Tanaka, H.; Uyama, H.; Kobayashi, S. Macromol. Rapid Commun. 1998, 19, 423. doi: 10.1002/(ISSN)1521-3927
20. [20]
  Nakao, L. S.; Kadiiska, M. B.; Mason, R. P.; Grijalba, M. T.; Augusto, O. Free Radical Biol. Med. 2000, 29, 721. doi: 10.1016/S0891-5849(00)00374-9
21. [21]
  Camarero, S.; Ibarra, D.; Martinez, M. J.; Martinez, A. T. Appl. Environ. Microbiol. 2005, 71, 1775. doi: 10.1128/AEM.71.4.1775-1784.2005
22. [22]
  Xu, F.; Kulys, J. J.; Duke, K.; Li, K. C.; Krikstopaitis, K.; Deussen, H. J. W.; Abbate, E.; Galinyte, V.; Schneider, P. Appl. Environ. Microbiol. 2000, 66, 2052. doi: 10.1128/AEM.66.5.2052-2056.2000
23. [23]
  Fabbrini, M.; Galli, C.; Gentili, P. J. Mol. Catal. B: Enzym. 2002, 16, 231. doi: 10.1016/S1381-1177(01)00067-4
1. [1]
  Peng XU , Shasha WANG , Nannan CHEN , Ao WANG , Dongmei YU . Preparation of three-layer magnetic composite Fe₃O₄@polyacrylic acid@ZiF-8 for efficient removal of malachite green in water. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 544-554. doi: 10.11862/CJIC.20230239
2. [2]
  Zhifeng CAI , Ying WU , Yanan LI , Guiyu MENG , Tianyu MIAO , Yihao ZHANG . Effective detection of malachite green by folic acid stabilized silver nanoclusters. Chinese Journal of Inorganic Chemistry, 2025, 41(5): 983-993. doi: 10.11862/CJIC.20240394
3. [3]
  Hongmei Chai , Yixia Ren , Xiangyang Hou , Long Tang , Jiawei Xie . 智能手机光传感的“丙酮碘化反应”实验改进. University Chemistry, 2025, 40(6): 193-200. doi: 10.12461/PKU.DXHX202407086
4. [4]
  Heng Zhang . Determination of All Rate Constants in the Enzyme Catalyzed Reactions Based on Michaelis-Menten Mechanism. University Chemistry, 2024, 39(4): 395-400. doi: 10.3866/PKU.DXHX202310047
5. [5]
  Yingxian Wang , Tianye Su , Limiao Shen , Jinping Gao , Qinghe Wu . Introduction of Chinese Lacquer from the Perspective of Chemistry: Popularizing Chemistry in Lacquer and Inherit Lacquer Art. University Chemistry, 2024, 39(5): 371-379. doi: 10.3866/PKU.DXHX202312015
6. [6]
  Yu Dai , Xueting Sun , Haoyu Wu , Naizhu Li , Guoe Cheng , Xiaojin Zhang , Fan Xia . Determination of the Michaelis Constant for Gold Nanozyme-Catalyzed Decomposition of Hydrogen Peroxide. University Chemistry, 2025, 40(5): 351-356. doi: 10.12461/PKU.DXHX202407052
7. [7]
  Zhongyan Cao , Shengnan Jin , Yuxia Wang , Yiyi Chen , Xianqiang Kong , Yuanqing Xu . Advances in Highly Selective Reactions Involving Phenol Derivatives as Aryl Radical Precursors. University Chemistry, 2025, 40(4): 245-252. doi: 10.12461/PKU.DXHX202405186
8. [8]
  Quanliang Chen , Zhaohui Zhou . Research on the Active Site of Nitrogenase over Fifty Years. University Chemistry, 2024, 39(7): 287-293. doi: 10.3866/PKU.DXHX202310133
9. [9]
  Yongqing Kuang , Jie Liu , Jianjun Feng , Wen Yang , Shuanglian Cai , Ling Shi . Experimental Design for the Two-Step Synthesis of Paracetamol from 4-Hydroxyacetophenone. University Chemistry, 2024, 39(8): 331-337. doi: 10.12461/PKU.DXHX202403012
10. [10]
  Xuanzhu Huo , Yixi Liu , Qiyu Wu , Zhiqiang Dong , Chanzi Ruan , Yanping Ren . Integrated Experiment of “Electrolytic Preparation of Cu₂O and Gasometric Determination of Avogadro’s Constant: Implementation, Results, and Discussion: A Micro-Experiment Recommended for Freshmen in Higher Education at Various Levels Across the Nation. University Chemistry, 2024, 39(3): 302-307. doi: 10.3866/PKU.DXHX202308095
11. [11]
  Bing LIU , Huang ZHANG , Hongliang HAN , Changwen HU , Yinglei ZHANG . Visible light degradation of methylene blue from water by triangle Au@TiO₂ mesoporous catalyst. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 941-952. doi: 10.11862/CJIC.20230398
12. [12]
  Lu XU , Chengyu ZHANG , Wenjuan JI , Haiying YANG , Yunlong FU . Zinc metal-organic framework with high-density free carboxyl oxygen functionalized pore walls for targeted electrochemical sensing of paracetamol. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 907-918. doi: 10.11862/CJIC.20230431
13. [13]
  Yuanpei ZHANG , Jiahong WANG , Jinming HUANG , Zhi HU . Preparation of magnetic mesoporous carbon loaded nano zero-valent iron for removal of Cr(Ⅲ) organic complexes from high-salt wastewater. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1731-1742. doi: 10.11862/CJIC.20240077
14. [14]
  Yinwu Su , Xuanwen Zheng , Jianghui Du , Boda Li , Tao Wang , Zhiyan Huang . Green Synthesis of 1,3-Dibromoacetone Using Halogen Exchange Method: Recommending a Basic Organic Synthesis Teaching Experiment. University Chemistry, 2024, 39(5): 307-314. doi: 10.3866/PKU.DXHX202311092
15. [15]
  Chunmei GUO , Weihan YIN , Jingyi SHI , Jianhang ZHAO , Ying CHEN , Quli FAN . Facile construction and peroxidase-like activity of single-atom platinum nanozyme. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1633-1639. doi: 10.11862/CJIC.20240162
16. [16]
  Min LIU , Huapeng RUAN , Zhongtao FENG , Xue DONG , Haiyan CUI , Xinping WANG . Neutral boron-containing radical dimers. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 123-130. doi: 10.11862/CJIC.20240362
17. [17]
  Liwei Wang , Guangran Ma , Li Wang , Fugang Xu . A Comprehensive Analytical Chemistry Experiment: Colorimetric Detection of Vitamin C Using Nanozyme and Smartphone. University Chemistry, 2024, 39(8): 255-262. doi: 10.3866/PKU.DXHX202312094
18. [18]
  Zhuoya WANG , Le HE , Zhiquan LIN , Yingxi WANG , Ling LI . Multifunctional nanozyme Prussian blue modified copper peroxide: Synthesis and photothermal enhanced catalytic therapy of self-provided hydrogen peroxide. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2445-2454. doi: 10.11862/CJIC.20240194
19. [19]
  Gonglan Ye , Xia Yin , Feng Xu , Peng Yang , Yingpeng Wu , Huilong Fei . Innovations in “Four-in-One” Inorganic Chemistry Education. University Chemistry, 2024, 39(8): 136-141. doi: 10.3866/PKU.DXHX202401071
20. [20]
  Xingyuan Lu , Yutao Yao , Junjing Gu , Peifeng Su . Energy Decomposition Analysis and Its Application in the Many-Body Effect of Water Clusters. University Chemistry, 2025, 40(3): 100-107. doi: 10.12461/PKU.DXHX202405074

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(696)
HTML views(66)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142