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Citation: ZHANG Qun, ZHANG Yu-Qi, LIU Xiao-Zhen, LIANG Yun-Xiao, ZHANG Rui-Feng, LONG Neng-Bing. Immobilization of Laccase on Large-Sized SiO2 Macroporous Materials[J]. Chinese Journal of Inorganic Chemistry, ;2013, 29(10): 2065-2070. doi: 10.3969/j.issn.1001-4861.2013.00.324 shu

Immobilization of Laccase on Large-Sized SiO2 Macroporous Materials

  • 1.

    State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China

  • Received Date: 22 April 2013
    Available Online: 29 May 2013

    Fund Project: 浙江省自然科学基金(No.LY12B01004) (No.LY12B01004)宁波市社会发展项目(No.2011C50052) (No.2011C50052)宁波大学王宽诚幸福基金(No.XKL072) (No.XKL072)先进能源材料化学教育部重点实验室(南开大学)开放课题(No.KLAEMC-OP201201)资助项目。 (南开大学)开放课题(No.KLAEMC-OP201201)

  • Alarge-sized SiO2 macroporous material modified by fixing Cu2+ on its surface was employed as the support for the immobilization of laccase. The effects of immobilization time, pHvalue and the amount of enzyme provided on the immobilization were investigated, and the activity and stability of the immobilized enzyme were also studied. The results show that the adsorption equilibrium is reached after about 5 hours. The optimum conditions for immobilization of laccase were at the immobilization pHof 4.5 and the ratio of enzyme to support of 5 mg·g-1, and in this case, the activity recovery of the immobilized laccase is 100.4%. Comparing with the free laccase, both the optimum working pHand temperature of the immobilized laccase are higher and the ranges are wider. The experiments show that the immobilization improves the pHstability and the thermal stability of laccase significantly. The Km of the immobilized laccase is higher than the free laccase's. The immobilized laccase has good operational stability, the remained activity is 72.7% after 10 times of reaction with the substrate.
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