Citation: GUO Ming, HUANG Feng-Qin, LIU Mi-Mi, LI Ming-Hui. Performance of Binding Interaction between Cr(Ⅵ) and Serum Proteome[J]. Chinese Journal of Inorganic Chemistry, ;2013, 29(5): 1037-1044. doi: 10.3969/j.issn.1001-4861.2013.00.159 shu

Performance of Binding Interaction between Cr(Ⅵ) and Serum Proteome

1.
Department of Chemistry, Zhejiang Agriculture & Forestry University, Lin'an, Zhejiang 311300, China

Corresponding author: GUO Ming,
Received Date: 25 December 2012
Available Online: 31 January 2013
Fund Project: 国家自然科学基金(No.20877072) (No.20877072)浙江科技厅公益项目(No.2012C37055) 资助项目。 (No.2012C37055)

The binding reaction between heavy metal ions Cr(Ⅵ) and serum proteome was investigated using affinity capillary electrophoresis(ACE) method. Based on the site binding model, a model about the interaction between Cr(Ⅵ) as the ligand and FBS as the receptor, was established. The binding performance of heavy metal ions Cr(Ⅵ) and the serum proteome was studied under proteomic conditions, and the results showed that, Cr(Ⅵ)-protein complexes formed between Cr(Ⅵ) and the high abundance component albumin in FBS, such as albumin (bovine serum albumin, BSA), transferrin protein (Bovine apo transferrin, bATF), immunogolobulin (Bovine IgG, IgG) and so on, and the binding reactions of Cr(Ⅵ) -FBS are fast equilibrium reactions. The apparent binding constant was determined through non-linear fitting binding reaction and based on changes of the effective mobility, and K_Cr(Ⅵ)-bATF=1.27×10⁴ L·mol^-1, K_Cr(Ⅵ)-IgG=4.91×10⁴ L·mol^-1, K_Cr(Ⅵ)-BSA=8.98×10⁴ L·mol^-1. Dosage-dependent effect exists among the concentration of Cr(Ⅵ) and the strength of the binding reactions. This work has referential meaning for understanding deeply the binding reaction mechanism of chromium(Ⅵ) with serum proteome.
- Cr(Ⅵ);serum proteome;apparent binding constant;complex;dosage-dependent effect
1. [1]
  [1] Jacobs J M, Adkins J N, Qian W J, et al. J. Proteome Res., 2005,4:1073-1085
2. [2]
  [2] Costa M. Toxicol. Appl. Pharmacol., 2003,188(1):1-5
3. [3]
  [3] Gray H B. Proc. Natl. Acad. Sci. USA, 2003,100(7):3563- 3568
4. [4]
  [4] WANG Rui(王锐), BAI Yan(白燕), LIANG Zhi-Hong(梁志红), et al. Acta Phys.-Chim. Sin.(Wuli Huaxue Xuebao), 2010,26 (12):3225-3229
5. [5]
  [5] Dai L B, Zhou C Z, Yuan S G. J. Chem. Eng. Chinese Universities, 2012,26(4):674-678
6. [6]
  [6] ZHANG Hai-Rong(张海蓉), BIAN He-Dong(边贺东), NI Shou-Hai(倪寿海), et al. Chinese J. Inorg. Chem. (Wuji Huaxue Xuebao), 2009,25(2):306-311
7. [7]
  [7] SHEN Xin-Can(沈星灿), BIAN He-Dong(边贺东), TU Chu- Qiao(涂楚桥), et al. Chinese J. Inorg. Chem. (Wuji Huaxue Xuebao), 2000,16(1):73-78
8. [8]
  [8] ZHANG Fang(张芳), LIN Dong-Hai(林东海). Chin. J. Magnet. Resonance (Bopuxue Zazhi), 2009,26(1):136-149
9. [9]
  [9] LIANG Yan-Qiu(梁彦秋), LIU Ting-Ting(刘婷婷), FEI Hong-Bo(费洪博), et al. J. Anal. Sci. (Fenxi Kexue Xuebao), 2007,23(3):303-306
10. [10]
  [10] Ye Q, Hu R, Lin Z Y, et al. Chem. J. Chinese Universities, 2006,27(8):1552-1554
11. [11]
  [11] Anwesha B, Kaushik B, Pradeep K S. J. Photochem. Photobiol. B: Biology, 2008,90(1):33-40
12. [12]
  [12] Auda S H, Mrestani Y, Ahmed A M S, et al. Electrophoresis, 2010,30(6):1066-1070
13. [13]
  [13] Rundlett K L, Armstrong D W. Electrophoresis, 2001,22(7): 1419-1427
14. [14]
  [14] Tanaka Y, Terebe S. J. Chromatogra. B, 2002,768(1):81-92
15. [15]
  [15] Urbaniak B, Mrestani Y, Kokot Z J, et al. Chromatographia, 2007,65(7/8):489-492
16. [16]
  [16] LIU Yong(刘勇), XIA Zhi-Ning(夏之宁), LI Hui(李晖). Chin. J. Anal. Chem.(Fenxi Huaxue), 2005,33(12):1733-1736
17. [17]
  [17] LI Bo(李博),LIU Wen-Ying(刘文英). Prog. Pharmaceut. Sci. (Yaoxue Jinzhan), 2003,27(2):81-84
18. [18]
  [18] Castagnola M, Rossetti D V, Tnzitari R, et al. Electrophoresis, 2003,24(5):801-807
19. [19]
  [19] Hu J, Lo M C, Chen G H. Sep. Purif. Technol., 2007,56(3): 249-256
20. [20]
  [20] YUN Qing-Xing(邢云青). Thesis for the Doctorate of Zhejiang University(浙江大学博士论文). 2007.
21. [21]
  [21] WANG Chang(王畅), ZHENG Yu-Fang(郑育芳), XIONG Jian-Hui (熊建辉), et al. Acta Chim. Sin. (Huaxue Xuebao), 2004,62(3):279-282
22. [22]
  [22] Deng B Y, Wang Y Z, Zhu P C, et al. Anal Chim. Acta, 2010,683(1): 58-62
23. [23]
  [23] Michael T, Bowser , David D. J. Phys. Chem., 1999,103(1): 197-202
24. [24]
  [24] Benjamin J, Cargile, Jonathan L, et al. J. Proteome Res., 2004, 3(1):112-119
25. [25]
  [25] Rosenfeld S I, Looney R J, Leddy J P. J. Chin. Invest, 1985,76(6):2317-2322
26. [26]
  [26] Saksena S, Zydney A L. Biotechnol. Bioengi., 1994,43(10): 960-968
27. [27]
  [27] GUO Ming(郭明), LIU Mi-Mi(刘咪咪), LI Ming-Hui(李铭慧), et al. Chin. J. Anal. Chem. (Fenxi Huaxue), 2012,40(2):268- 272
28. [28]
  [28] Zhang P J, Lan P, Ma Y N , et al. J. Biochem. Mol. Toxicol., 2012,26(2):54-59
29. [29]
  [29] LI Xiao-Feng(李晓峰). Thesis for the Masterate of Tianjin University(天津大学硕士论文), 2003.
30. [30]
  [30] YU Wen-Peng(于文鹏). Thesis for the Masterate of Beijing Forestry University (北京林业大学硕士论文). 2005.
31. [31]
  [31] Michael V, Ellen V, Roland B. J. Proteomics, 2012,75(18): 5848-5860
32. [32]
  [31] YUAN Pei(袁佩), XIA Zhi-Ning(夏之宁), LIU Yong(刘勇). Chin. J. Instrument. Anal.(Fenxi Ceshi Xuebao), 2001,20(1): 79-83
33. [33]
  [32] Liu Y, Zhang S Y, Ling X M, et al. J. Peptide Sci., 2008,14 (8):984-988
34. [34]
  [33] Allen S J, Crown S E, Handel T M. Annu. Rev. Immunol., 2007,25(1):787-820
35. [35]
  [34] Kraly J, Fazal M A, Schoenherr R M, et al. Anal. Chem., 2006,78(1):4097-4110
36. [36]
  [35] ZHAO Yan-Fang(赵艳芳), FU Chong-Gong(傅崇岗), LIU Ai-Lin(刘爱林). Chin. J. Chromatography (Sepu), 2003,21 (2):126-130
37. [37]
  [36] Michael V, Ellen V, Roland B. J. Proteomics, 2012,75(18): 5848-5860
38. [38]
  [37] Dudev T, Lim C. Annu. Rev. Biophys., 2008,37(1):97-116
39. [39]
  [38] HE Wen-Ying(何文英), YAO Xiao-Jun(姚小军), LIU Peng- Jun(刘鹏军), et al. Sci. China Chem.(ZhongguoKexue), 2007,37(1):54-63
1. [1]
  Jie ZHANG , Xin LIU , Zhixin LI , Yuting PEI , Yuqi YANG , Huimin LI , Zhiqiang LIU . Assembling a luminescence silencing system based on post-synthetic modification strategy: A highly sensitive and selective turn-on metal-organic framework probe for ascorbic acid detection. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 823-833. doi: 10.11862/CJIC.20230310
2. [2]
  Yuanpei ZHANG , Jiahong WANG , Jinming HUANG , Zhi HU . Preparation of magnetic mesoporous carbon loaded nano zero-valent iron for removal of Cr(Ⅲ) organic complexes from high-salt wastewater. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1731-1742. doi: 10.11862/CJIC.20240077
3. [3]
  Zhongsen Wang , Lijun Qiu , Yunhua Huang , Meng Zhang , Xi Cai , Fanyu Wang , Yang Lin , Yanbiao Shi , Xiao Liu . Alcohothermal synthesis of sulfidated zero-valent iron for enhanced Cr(Ⅵ) removal. Chinese Chemical Letters, 2024, 35(7): 109195-. doi: 10.1016/j.cclet.2023.109195
4. [4]
  Shuqi Yu , Yu Yang , Keisuke Kuroda , Jian Pu , Rui Guo , Li-An Hou . Selective removal of Cr(Ⅵ) using polyvinylpyrrolidone and polyacrylamide co-modified MoS₂ composites by adsorption combined with reduction. Chinese Chemical Letters, 2024, 35(6): 109130-. doi: 10.1016/j.cclet.2023.109130
5. [5]
  Ting-Ting Huang , Jin-Fa Chen , Juan Liu , Tai-Bao Wei , Hong Yao , Bingbing Shi , Qi Lin . A novel fused bi-macrocyclic host for sensitive detection of Cr₂O₇²⁻ based on enrichment effect. Chinese Chemical Letters, 2024, 35(7): 109281-. doi: 10.1016/j.cclet.2023.109281
6. [6]
  Ya-Nan Yang , Zi-Sheng Li , Sourav Mondal , Lei Qiao , Cui-Cui Wang , Wen-Juan Tian , Zhong-Ming Sun , John E. McGrady . Metal-metal bonds in Zintl clusters: Synthesis, structure and bonding in [Fe₂Sn₄Bi₈]^3– and [Cr₂Sb₁₂]^3–. Chinese Chemical Letters, 2024, 35(8): 109048-. doi: 10.1016/j.cclet.2023.109048
7. [7]
  Tong Zhou , Xue Liu , Liang Zhao , Mingtao Qiao , Wanying Lei . Efficient Photocatalytic H₂O₂ Production and Cr(VI) Reduction over a Hierarchical Ti₃C₂/In₄SnS₈ Schottky Junction. Acta Physico-Chimica Sinica, 2024, 40(10): 2309020-. doi: 10.3866/PKU.WHXB202309020
8. [8]
  Zongfei YANG , Xiaosen ZHAO , Jing LI , Wenchang ZHUANG . Research advances in heteropolyoxoniobates. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 465-480. doi: 10.11862/CJIC.20230306
9. [9]
  Qilu DU , Li ZHAO , Peng NIE , Bo XU . Synthesis and characterization of osmium-germyl complexes stabilized by triphenyl ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1088-1094. doi: 10.11862/CJIC.20240006
10. [10]
  Haitang WANG , Yanni LING , Xiaqing MA , Yuxin CHEN , Rui ZHANG , Keyi WANG , Ying ZHANG , Wenmin WANG . Construction, crystal structures, and biological activities of two Ln^Ⅲ₃ complexes. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1474-1482. doi: 10.11862/CJIC.20240188
11. [11]
  Chuanming GUO , Kaiyang ZHANG , Yun WU , Rui YAO , Qiang ZHAO , Jinping LI , Guang LIU . Performance of MnO₂-0.39IrO_x composite oxides for water oxidation reaction in acidic media. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1135-1142. doi: 10.11862/CJIC.20230459
12. [12]
  Jingjing QING , Fan HE , Zhihui LIU , Shuaipeng HOU , Ya LIU , Yifan JIANG , Mengting TAN , Lifang HE , Fuxing ZHANG , Xiaoming ZHU . Synthesis, structure, and anticancer activity of two complexes of dimethylglyoxime organotin. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1301-1308. doi: 10.11862/CJIC.20240003
13. [13]
  Zeyu XU , Anlei DANG , Bihua DENG , Xiaoxin ZUO , Yu LU , Ping YANG , Wenzhu YIN . Evaluation of the efficacy of graphene oxide quantum dots as an ovalbumin delivery platform and adjuvant for immune enhancement. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1065-1078. doi: 10.11862/CJIC.20240099
14. [14]
  Endong YANG , Haoze TIAN , Ke ZHANG , Yongbing LOU . Efficient oxygen evolution reaction of CuCo₂O₄/NiFe-layered bimetallic hydroxide core-shell nanoflower sphere arrays. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 930-940. doi: 10.11862/CJIC.20230369
15. [15]
  Qiangqiang SUN , Pengcheng ZHAO , Ruoyu WU , Baoyue CAO . Multistage microporous bifunctional catalyst constructed by P-doped nickel-based sulfide ultra-thin nanosheets for energy-efficient hydrogen production from water electrolysis. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1151-1161. doi: 10.11862/CJIC.20230454
16. [16]
  Xin MA , Ya SUN , Na SUN , Qian KANG , Jiajia ZHANG , Ruitao ZHU , Xiaoli GAO . A Tb₂ complex based on polydentate Schiff base: Crystal structure, fluorescence properties, and biological activity. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1347-1356. doi: 10.11862/CJIC.20230357
17. [17]
  Yingchun ZHANG , Yiwei SHI , Ruijie YANG , Xin WANG , Zhiguo SONG , Min WANG . Dual ligands manganese complexes based on benzene sulfonic acid and 2, 2′-bipyridine: Structure and catalytic properties and mechanism in Mannich reaction. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1501-1510. doi: 10.11862/CJIC.20240078
18. [18]
  Zhaoyang WANG , Chun YANG , Yaoyao Song , Na HAN , Xiaomeng LIU , Qinglun WANG . Lanthanide(Ⅲ) complexes derived from 4′-(2-pyridyl)-2, 2′∶6′, 2″-terpyridine: Crystal structures, fluorescent and magnetic properties. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1442-1451. doi: 10.11862/CJIC.20240114
19. [19]
  Xinting XIONG , Zhiqiang XIONG , Panlei XIAO , Xuliang NIE , Xiuying SONG , Xiuguang YI . Synthesis, crystal structures, Hirshfeld surface analysis, and antifungal activity of two complexes Na(Ⅰ)/Cd(Ⅱ) assembled by 5-bromo-2-hydroxybenzoic acid ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1661-1670. doi: 10.11862/CJIC.20240145
20. [20]
  Yan LIU , Jiaxin GUO , Song YANG , Shixian XU , Yanyan YANG , Zhongliang YU , Xiaogang HAO . Exclusionary recovery of phosphate anions with low concentration from wastewater using a CoNi-layered double hydroxide/graphene electronically controlled separation film. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1775-1783. doi: 10.11862/CJIC.20240043

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(238)
HTML views(16)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142