Citation: TANG Jing-Long, WANG Shuo, LIU Li, WANG Chun-Ren, XI Ting-Fei. A Preliminary Study on the Dose-Effect Relation when Silver Nanoparticles Crossing through the Blood-Brain Barrier in vitro[J]. Chinese Journal of Inorganic Chemistry, ;2013, 29(5): 1025-1030. doi: 10.3969/j.issn.1001-4861.2013.00.150 shu

A Preliminary Study on the Dose-Effect Relation when Silver Nanoparticles Crossing through the Blood-Brain Barrier in vitro

1.
1. National Institutes for Food and Drug Control, Beijing 100050, China;

Corresponding author: TANG Jing-Long,
Received Date: 25 December 2012
Available Online: 21 January 2013
Fund Project: 北京市自然科学基金(No.3112024) (No.3112024)中国食品药品检定研究院中青年发展研究基金(No.2009C4)资助项目。 (No.2009C4)

Objective:The aim of this study was to investigate the dose-effect relation of the ability that silver nanoparticles (SNPs) crossing through the blood-brain barrier (BBB) and presume its mechanism. Methods:In this study, SNPs were test samples and silver microparticles (SMPs) acted as control samples. First, an in vitro BBB model was established. Second, SNPs or SMPs were cultured in the BBB model at different dose from 25 μg·mL^-1 to 400 μg·mL^-1, respectively. After 4 hours of culture, the ultrastructure of BBB and the percentage of silver particles crossing through BBB were evaluated with TEM and ICP-MS respectively. Results:Results demonstrated that SNPs crossed the BBB, while the SMPs did not. When SNPS dose<100 μg·mL^-1, about 2% silver particles crossed BBB, and the BBBs ultrastructures were normal. When dose>100 μg·mL^-1, the higher dose is, the more silver particles crossed BBB, and the more damage occurred in the BBBs ultrastructures. When dose=400 μg·mL^-1, about 15% silver particles crossed BBB. Conclusion:The results suggested that membrane-mobile transport mechanism is the main way allowing SNPs to cross the BBB in low dose. Cytotoxicity mechanism is the main way allowing SNPs to cross the BBB in higher dose. It is also suggested that SNPs could cross the BBB even in a low dose. A cautious attitude would be hold before silver nanoparticles-based medical devices were used in clinical practice.
- silver nanoparticle;blood-brain barrier;dose-effect relation
1. [1]
  [1] Chen X, Schluesener H J. Toxicol Lett., 2008,176(1):1-12
2. [2]
  [2] Martinez-Castanon G A, Nino-Martinez N, Martinez-Guiterrez F, et al. J. Nanoparticle Res., 2008,10(8):1343-1348
3. [3]
  [3] Estores I M, Olsen D, Gómez-Marin O. J Rehabil Res Dev., 2008,45(1):135-139
4. [4]
  [4] Vlachou E, Chipp E, Shale E, et al. Burns., 2007,3(8):979- 985
5. [5]
  [5] Roe D, Karandikar B, Bonn-Savage N, et al. J Antimicrob Chemother., 2008,61(4):869-876
6. [6]
  [6] Takenaka S, Karg E, Roth C, et al. Environ. Health Perspect., 2001,109(Suppl 4):547-551
7. [7]
  [7] Tang J L, Xiong L, Wang S, et al. J. Nanosci. Nanotechnol., 2009,9(8):4924-4932
8. [8]
  [8] TANG Jing-Long(汤京龙), XI Ting-Fei(奚廷斐), WEI Li-Na (魏丽娜), et al. Chinese J. Inorg. Chem. (Wuji Huaxue Xuebao), 2008,24(11):1827-1831
9. [9]
  [9] Tang J L, Xiong L, Wang S, et al. Appl. Surface Sci., 2008,255(2):502-504
10. [10]
  [10] TANG Jing-Long(汤京龙), XI Ting-Fei(奚廷斐), XIONG Ling(熊玲),et al. Transact. Mater. Heat Treat. (Cailiao Rechuli Xuebao), 2009,30(3):6-9
11. [11]
  [11] Tang J L, Xiong L, Zhou G F,et al. J. Nanosci. Nanotechnol., 2010,10(10):6313-6317
12. [12]
  [12] CHENG Ling-Zhong(成令钟), ZHONG Cui-Ping(钟翠萍), CAI Wen-Qin(蔡文琴). Contemporary Histology(现代组织学). Shanghai: Scientific and Technological Literature Publishing House, 2003.
13. [13]
  [13] U.S. Department of Health and Human Services, Food and Drug Administration. Guidance for Industry and FDA Premarket and Design Control Reviewers Medical Device Use-Safety: Incorporating Human Factors Engineering into Risk Management[N]. [2011-08-20]. http://www.fda.gov/ MedicalDevices/ DeviceRegulationand Guidance/Guidance Documents/ucm193096.htm
14. [14]
  [14] State Food and Drug Administration(国家食品药品监督管理局). YY/T 0316-2008 Medical devices Application of risk management to medical devices [S]. Beijing: Standards Press of China, 2009.
1. [1]
  Yuhao SUN , Qingzhe DONG , Lei ZHAO , Xiaodan JIANG , Hailing GUO , Xianglong MENG , Yongmei GUO . Synthesis and antibacterial properties of silver-loaded sod-based zeolite. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 761-770. doi: 10.11862/CJIC.20230169
2. [2]
  Qin ZHU , Jiao MA , Zhihui QIAN , Yuxu LUO , Yujiao GUO , Mingwu XIANG , Xiaofang LIU , Ping NING , Junming GUO . Morphological evolution and electrochemical properties of cathode material LiAl_0.08Mn_1.92O₄ single crystal particles. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1549-1562. doi: 10.11862/CJIC.20240022
3. [3]
  Cheng PENG , Jianwei WEI , Yating CHEN , Nan HU , Hui ZENG . First principles investigation about interference effects of electronic and optical properties of inorganic and lead-free perovskite Cs₃Bi₂X₉ (X=Cl, Br, I). Chinese Journal of Inorganic Chemistry, 2024, 40(3): 555-560. doi: 10.11862/CJIC.20230282
4. [4]
  Kexin Dong , Chuqi Shen , Ruyu Yan , Yanping Liu , Chunqiang Zhuang , Shijie Li . Integration of Plasmonic Effect and S-Scheme Heterojunction into Ag/Ag₃PO₄/C₃N₅ Photocatalyst for Boosted Photocatalytic Levofloxacin Degradation. Acta Physico-Chimica Sinica, 2024, 40(10): 2310013-. doi: 10.3866/PKU.WHXB202310013
5. [5]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403
6. [6]
  Hong LI , Xiaoying DING , Cihang LIU , Jinghan ZHANG , Yanying RAO . Detection of iron and copper ions based on gold nanorod etching colorimetry. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 953-962. doi: 10.11862/CJIC.20230370
7. [7]
  Guimin ZHANG , Wenjuan MA , Wenqiang DING , Zhengyi FU . Synthesis and catalytic properties of hollow AgPd bimetallic nanospheres. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 963-971. doi: 10.11862/CJIC.20230293
8. [8]
  Jingke LIU , Jia CHEN , Yingchao HAN . Nano hydroxyapatite stable suspension system: Preparation and cobalt adsorption performance. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1763-1774. doi: 10.11862/CJIC.20240060
9. [9]
  Wenjiang LI , Pingli GUAN , Rui YU , Yuansheng CHENG , Xianwen WEI . C₆₀-MoP-C nanoflowers van der Waals heterojunctions and its electrocatalytic hydrogen evolution performance. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 771-781. doi: 10.11862/CJIC.20230289
10. [10]
  Peng ZHOU , Xiao CAI , Qingxiang MA , Xu LIU . Effects of Cu doping on the structure and optical properties of Au₁₁(dppf)₄Cl₂ nanocluster. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1254-1260. doi: 10.11862/CJIC.20240047
11. [11]
  Siyu HOU , Weiyao LI , Jiadong LIU , Fei WANG , Wensi LIU , Jing YANG , Ying ZHANG . Preparation and catalytic performance of magnetic nano iron oxide by oxidation co-precipitation method. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1577-1582. doi: 10.11862/CJIC.20230469
12. [12]
  Guangming YIN , Huaiyao WANG , Jianhua ZHENG , Xinyue DONG , Jian LI , Yi'nan SUN , Yiming GAO , Bingbing WANG . Preparation and photocatalytic degradation performance of Ag/protonated g-C₃N₄ nanorod materials. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1491-1500. doi: 10.11862/CJIC.20240086
13. [13]
  Qi Li , Pingan Li , Zetong Liu , Jiahui Zhang , Hao Zhang , Weilai Yu , Xianluo Hu . Fabricating Micro/Nanostructured Separators and Electrode Materials by Coaxial Electrospinning for Lithium-Ion Batteries: From Fundamentals to Applications. Acta Physico-Chimica Sinica, 2024, 40(10): 2311030-. doi: 10.3866/PKU.WHXB202311030
14. [14]
  Di WU , Ruimeng SHI , Zhaoyang WANG , Yuehua SHI , Fan YANG , Leyong ZENG . Construction of pH/photothermal dual-responsive delivery nanosystem for combination therapy of drug-resistant bladder cancer cell. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1679-1688. doi: 10.11862/CJIC.20240135
15. [15]
  Chunmei GUO , Weihan YIN , Jingyi SHI , Jianhang ZHAO , Ying CHEN , Quli FAN . Facile construction and peroxidase-like activity of single-atom platinum nanozyme. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1633-1639. doi: 10.11862/CJIC.20240162
16. [16]
  Qingtang ZHANG , Xiaoyu WU , Zheng WANG , Xiaomei WANG . Performance of nano Li₂FeSiO₄/C cathode material co-doped by potassium and chlorine ions. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1689-1696. doi: 10.11862/CJIC.20240115
17. [17]
  Juan WANG , Zhongqiu WANG , Qin SHANG , Guohong WANG , Jinmao LI . NiS and Pt as dual co-catalysts for the enhanced photocatalytic H₂ production activity of BaTiO₃ nanofibers. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1719-1730. doi: 10.11862/CJIC.20240102
18. [18]
  Endong YANG , Haoze TIAN , Ke ZHANG , Yongbing LOU . Efficient oxygen evolution reaction of CuCo₂O₄/NiFe-layered bimetallic hydroxide core-shell nanoflower sphere arrays. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 930-940. doi: 10.11862/CJIC.20230369
19. [19]
  Hailang JIA , Hongcheng LI , Pengcheng JI , Yang TENG , Mingyun GUAN . Preparation and performance of N-doped carbon nanotubes composite Co₃O₄ as oxygen reduction reaction electrocatalysts. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 693-700. doi: 10.11862/CJIC.20230402
20. [20]
  Yufang GAO , Nan HOU , Yaning LIANG , Ning LI , Yanting ZHANG , Zelong LI , Xiaofeng LI . Nano-thin layer MCM-22 zeolite: Synthesis and catalytic properties of trimethylbenzene isomerization reaction. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1079-1087. doi: 10.11862/CJIC.20240036

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(170)
HTML views(11)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142