Citation: WANG Lei, CHEN Cheng, TIAN Mei-Juan, YANG Yu-Xiang, LUO Jian-Hui, DING Bin, WANG Ping-Mei. Modified Mesoporous Silica: Synthesis and Immobilization Function for Novozymes Laccase[J]. Chinese Journal of Inorganic Chemistry, ;2013, 29(4): 677-688. doi: 10.3969/j.issn.1001-4861.2013.00.118
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Mesoporous silica was prepared by using hexadecyl trimethyl ammonium bromide (CTAB) as the template, tetraethoxysilane (tetraethylorthosilicate, TEOS) as the silicon source and nitric acid as the hydrolysis catalyst. The obtained sample was then modified via post grafting method. The mesoporous silica before and after modification was characterized by IR, DTA-TG, XRD, SEM, elemental analysis, micro-electrophoresis and N2 adsorption-desorption. The results show that the mesoporous silica has been modified by amino-groups, the material after modification still belongs to a family of mesoporous materials in spite of a slight decrease in the order degree. The surface area, pore size and pore volume become smaller, and the isoelectric point is decreased from 2.74 to 4.75 after the modification. The commercial Novozymes laccase is successfully immobilized in the inner surface of amino-functionalized mesoporous silica in this work by using glutaraldehyde as the crosslinking agent. The conditions of immobilization on amino-functionalized mesoporous silica were optimized by means of orthogonal experimental designs. The results show that the operational stability of the immobilized enzyme is significantly improved in comparison with the free enzyme.
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