Citation: XU Li, CHEN Yu, WU Jia-Huan, WEN Ban-Kang. DNA Interaction and Antitumor Activities of Ruthenium(Ⅱ) Polypyridyl Complex[J]. Chinese Journal of Inorganic Chemistry, ;2013, 29(3): 613-620. doi: 10.3969/j.issn.1001-4861.2013.00.107
-
The interactions of the Ru(Ⅱ) complex, [Ru(phen)2(Hecip)]2+ (phen=1,10-phenanthroline, Hecip=N-ethyl-4-([1,10]-phenanthroline[5,6-f]imidazol-2-yl)carbazole), with calf thymus DNA (CT DNA) were studied by using absorption spectroscopy, binding stoichiometry, viscosity measurement and photoactivated cleavage. A tight 2:1 complex is formed by the Ru(Ⅱ) polypyridyl complex and CT DNA with a binding constant exceeding 105 mol-1·L and with a binding mode of intercalation. Furthermore, the complex exhibits efficient DNA cleavage activity on UV (365 nm) irradiation via a mechanistic pathway involving formation of singlet oxygen as the reactive species. On the other hand, the cytotoxic activity of the complex was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) method. The complex shows prominent anticancer activity against selected tumor cell lines with IC50 values lower than those of cisplatin. Further flow cytometry experiments show that the cytotoxic Ru(Ⅱ) complex can cause cell cycle arrest in the S phase.
-
-
[1]
[1] Greguric I, Aldrich-Wright J R, Collins J G. J. Am. Chem. Soc., 1997,119:3621-3622
-
[2]
[2] Nair R B, Teng E S, Kirkland S L, et al. Inorg. Chem., 1998, 37:139-141
-
[3]
[3] Erkkila K E, Odom D T, Barton J K. Chem. Rev., 1999,99: 2777-2796
-
[4]
[4] Friedman A E, Chambron J C, Sauvage J P, et al. J. Am. Chem. Soc., 2004,126:8630-8631
-
[5]
[5] Zeglis B M, Barton J K. J. Am. Chem. Soc., 2006,128:5654-5655
-
[6]
[6] Liu Y, Hammitt R, Lutterman D A, et al. Inorg. Chem., 2007, 46:6011-6021
-
[7]
[7] Cosgrave L, Devocelle M, Forster R J, et al. Chem. Commun., 2010,46:103-105
-
[8]
[8] Tan C P, Wu S H, Lai S S, et al. Dalton Trans., 2011,40: 8611-8621
-
[9]
[9] Tan L F, Shen J L, Liu J, et al. Dalton Trans., 2012,41:4575-4587
-
[10]
[10] Liu Y J, Li Z Z, Liang Z H, et al. DNA Cell Biol., 2011,30: 839-848
-
[11]
[11] Tan C P, Lai S S, Wu S H, et al. J. Med. Chem., 2010,53: 7613-7624
-
[12]
[12] Schatzschneider U, Niesel J, Ott I, et al. ChemMedChem, 2008,3:1104-1109
-
[13]
[13] Van Dijken A, Bastiaansen J J A M, Kiggen N M M, et al. J. Am. Chem. Soc., 2004,126:7718-7727
-
[14]
[14] Grabowski Z R, Rotkiewicz K. Chem. Rev., 2003,103:3899-4032
-
[15]
[15] Zhang Y, Wang L, Wada T, et al. Macromol. Chem. Phys., 1996,197:1877-1888
-
[16]
[16] Wagner J, Pielichowski J, Hinsch A, et al. Synth. Met., 2004,146:159-165
-
[17]
[17] Xin H, Sun M, Wang K Z, et al. Chem. Phys. Lett., 2004, 388:55-57
-
[18]
[18] Liu F R, Wang K Z, Bai G Y, et al. Inorg. Chem., 2004,43: 1799-1806
-
[19]
[19] Lü Y Y, Gao L H, Han M J, et al. Eur. J. Inorg. Chem., 2006,430:430-436
-
[20]
[20] Xu L, Liu P X, Liao G L, et al. Aust. J. Chem., 2010,63:1-9
-
[21]
[21] Marmur J A. J. Mol. Biol., 1961,3:208-218
-
[22]
[22] Reichmann M E, Rice S A, Thomas C A, et al. J. Am. Chem. Soc., 1954,76:3047-3053
-
[23]
[23] Wolf A, Shimer Jr G H, Meehan T. Biochemistry, 1987,26: 6392-6396
-
[24]
[24] Chaires J B, Dattagupta N, Crothers D M. Biochemistry, 1982,21:3933-3940
-
[25]
[25] Cohen G, Eisenberg H. Biopolymers, 1969,8:45-55
-
[26]
[26] Job P. Ann. Chim., 1928,9:113-203
-
[27]
[27] Mosmann T. J. Immunol. Methods., 1983,65:55-63
-
[28]
[28] Tan L F, Song F C, Zou X Q, et al. DNA Cell Biol., 2011, 30:277-285
-
[29]
[29] Pyle A M, Rehmann J P, Meshoyrer R, et al. J. Am. Chem. Soc., 1989,111:3051-3058
-
[30]
[30] Han M J, Duan Z M, Wang K Z, et al. J. Phys. Chem. C., 2007,111:16577-16585
-
[31]
[31] Friedman A E, Chambron J C, Sauvage J P, et al. J. Am. Chem. Soc., 1990,112:4960-4962
-
[32]
[32] Tselepi-Kalouli E, Katsaros N. J. Inorg. Biochem., 1989,37: 271-282
-
[33]
[33] Liu J G, Zhang Q L, Shi X F, et al. Inorg. Chem., 2001,40: 5045-5050
-
[34]
[34] Satyanarayana S, Dabrowiak J C, Chaires J B. Biochemistry, 1992,31:9319-9324
-
[35]
[35] Satyanaryana S, Daborusak J C, Chaires J B. Biochem., 1993,32:2573-2584
-
[36]
[36] Cheng C C, Rokita S E, Burrows C J. Angew. Chem. Int. Ed. Engl., 1993,32:277-278
-
[37]
[37] Lesko S A, Lorentzen R J, Ts'o P O. Biochemistry, 1980,19: 3023-3028
-
[38]
[38] Nilsson R, Merkel P B, Kearns D R. Photochem. Photobiol., 1972,16:117-124
-
[39]
[39] Patra A K, Nethaji M, Chakravarty A R. J. Inorg. Biochem., 2007,101:233-244
-
[40]
[40] Deshpande M S, Kumbhar A A, Kumbhar A S, et al. Bioconjugate Chem., 2009,20:447-459
-
[41]
[41] Gao F, Chao H, Ji L N. Chem. Biodivers., 2008,5:1962-1979
-
[42]
[42] Yu H J, Chen Y, Yu L, et al. Eur. J. Med. Chem., 2012,55: 146-154
-
[43]
[43] Karna P, Sharp S M, Yates C, et al. Mol. Cancer., 2009,8: 93
-
[1]
-
-
[1]
Qilu DU , Li ZHAO , Peng NIE , Bo XU . Synthesis and characterization of osmium-germyl complexes stabilized by triphenyl ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1088-1094. doi: 10.11862/CJIC.20240006
-
[2]
Haitang WANG , Yanni LING , Xiaqing MA , Yuxin CHEN , Rui ZHANG , Keyi WANG , Ying ZHANG , Wenmin WANG . Construction, crystal structures, and biological activities of two LnⅢ3 complexes. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1474-1482. doi: 10.11862/CJIC.20240188
-
[3]
Jingjing QING , Fan HE , Zhihui LIU , Shuaipeng HOU , Ya LIU , Yifan JIANG , Mengting TAN , Lifang HE , Fuxing ZHANG , Xiaoming ZHU . Synthesis, structure, and anticancer activity of two complexes of dimethylglyoxime organotin. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1301-1308. doi: 10.11862/CJIC.20240003
-
[4]
Xin MA , Ya SUN , Na SUN , Qian KANG , Jiajia ZHANG , Ruitao ZHU , Xiaoli GAO . A Tb2 complex based on polydentate Schiff base: Crystal structure, fluorescence properties, and biological activity. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1347-1356. doi: 10.11862/CJIC.20230357
-
[5]
Yingchun ZHANG , Yiwei SHI , Ruijie YANG , Xin WANG , Zhiguo SONG , Min WANG . Dual ligands manganese complexes based on benzene sulfonic acid and 2, 2′-bipyridine: Structure and catalytic properties and mechanism in Mannich reaction. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1501-1510. doi: 10.11862/CJIC.20240078
-
[6]
Zhaoyang WANG , Chun YANG , Yaoyao Song , Na HAN , Xiaomeng LIU , Qinglun WANG . Lanthanide(Ⅲ) complexes derived from 4′-(2-pyridyl)-2, 2′∶6′, 2″-terpyridine: Crystal structures, fluorescent and magnetic properties. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1442-1451. doi: 10.11862/CJIC.20240114
-
[7]
Xinting XIONG , Zhiqiang XIONG , Panlei XIAO , Xuliang NIE , Xiuying SONG , Xiuguang YI . Synthesis, crystal structures, Hirshfeld surface analysis, and antifungal activity of two complexes Na(Ⅰ)/Cd(Ⅱ) assembled by 5-bromo-2-hydroxybenzoic acid ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1661-1670. doi: 10.11862/CJIC.20240145
-
[8]
Yuanpei ZHANG , Jiahong WANG , Jinming HUANG , Zhi HU . Preparation of magnetic mesoporous carbon loaded nano zero-valent iron for removal of Cr(Ⅲ) organic complexes from high-salt wastewater. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1731-1742. doi: 10.11862/CJIC.20240077
-
[9]
Chang Liu , Tao Wu , Lijiao Deng , Xuzi Li , Xin Fu , Shuzhen Liao , Wenjie Ma , Guoqiang Zou , Hai Yang . Programmed DNA walkers for biosensors. Chinese Chemical Letters, 2024, 35(9): 109307-. doi: 10.1016/j.cclet.2023.109307
-
[10]
Jia-Li Xie , Tian-Jin Xie , Yu-Jie Luo , Kai Mao , Cheng-Zhi Huang , Yuan-Fang Li , Shu-Jun Zhen . Octopus-like DNA nanostructure coupled with graphene oxide enhanced fluorescence anisotropy for hepatitis B virus DNA detection. Chinese Chemical Letters, 2024, 35(6): 109137-. doi: 10.1016/j.cclet.2023.109137
-
[11]
Jiaqi AN , Yunle LIU , Jianxuan SHANG , Yan GUO , Ce LIU , Fanlong ZENG , Anyang LI , Wenyuan WANG . Reactivity of extremely bulky silylaminogermylene chloride and bonding analysis of a cubic tetragermylene. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1511-1518. doi: 10.11862/CJIC.20240072
-
[12]
Yang Qin , Jiangtian Li , Xuehao Zhang , Kaixuan Wan , Heao Zhang , Feiyang Huang , Limei Wang , Hongxun Wang , Longjie Li , Xianjin Xiao . Toeless and reversible DNA strand displacement based on Hoogsteen-bond triplex. Chinese Chemical Letters, 2024, 35(5): 108826-. doi: 10.1016/j.cclet.2023.108826
-
[13]
Xiaohong Wen , Mei Yang , Lie Li , Mingmin Huang , Wei Cui , Suping Li , Haiyan Chen , Chen Li , Qiuping Guo . Enzymatically controlled DNA tetrahedron nanoprobes for specific imaging of ATP in tumor. Chinese Chemical Letters, 2024, 35(8): 109291-. doi: 10.1016/j.cclet.2023.109291
-
[14]
Jingwen Zhao , Jianpu Tang , Zhen Cui , Limin Liu , Dayong Yang , Chi Yao . A DNA micro-complex containing polyaptamer for exosome separation and wound healing. Chinese Chemical Letters, 2024, 35(9): 109303-. doi: 10.1016/j.cclet.2023.109303
-
[15]
Zongfei YANG , Xiaosen ZHAO , Jing LI , Wenchang ZHUANG . Research advances in heteropolyoxoniobates. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 465-480. doi: 10.11862/CJIC.20230306
-
[16]
Tian Feng , Yun-Ling Gao , Di Hu , Ke-Yu Yuan , Shu-Yi Gu , Yao-Hua Gu , Si-Yu Yu , Jun Xiong , Yu-Qi Feng , Jie Wang , Bi-Feng Yuan . Chronic sleep deprivation induces alterations in DNA and RNA modifications by liquid chromatography-mass spectrometry analysis. Chinese Chemical Letters, 2024, 35(8): 109259-. doi: 10.1016/j.cclet.2023.109259
-
[17]
Zhe-Han Yang , Jie Yin , Lei Xin , Yuanfang Li , Yijie Huang , Ruo Yuan , Ying Zhuo . Research advancement of DNA-based intelligent hydrogels: Manufacture, characteristics, application of disease diagnosis and treatment. Chinese Chemical Letters, 2024, 35(10): 109558-. doi: 10.1016/j.cclet.2024.109558
-
[18]
Siyi ZHONG , Xiaowen LIN , Jiaxin LIU , Ruyi WANG , Tao LIANG , Zhengfeng DENG , Ao ZHONG , Cuiping HAN . Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1483-1490. doi: 10.11862/CJIC.20240093
-
[19]
Di WU , Ruimeng SHI , Zhaoyang WANG , Yuehua SHI , Fan YANG , Leyong ZENG . Construction of pH/photothermal dual-responsive delivery nanosystem for combination therapy of drug-resistant bladder cancer cell. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1679-1688. doi: 10.11862/CJIC.20240135
-
[20]
Chuanming GUO , Kaiyang ZHANG , Yun WU , Rui YAO , Qiang ZHAO , Jinping LI , Guang LIU . Performance of MnO2-0.39IrOx composite oxides for water oxidation reaction in acidic media. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1135-1142. doi: 10.11862/CJIC.20230459
-
[1]
Metrics
- PDF Downloads(0)
- Abstract views(279)
- HTML views(52)