Citation: XU Li, CHEN Yu, WU Jia-Huan, WEN Ban-Kang. DNA Interaction and Antitumor Activities of Ruthenium(Ⅱ) Polypyridyl Complex[J]. Chinese Journal of Inorganic Chemistry, ;2013, 29(3): 613-620. doi: 10.3969/j.issn.1001-4861.2013.00.107 shu

DNA Interaction and Antitumor Activities of Ruthenium(Ⅱ) Polypyridyl Complex

XU Li ¹ ,
CHEN Yu ² ,
WU Jia-Huan ¹ ,
WEN Ban-Kang ¹

1.
1. School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Zhongshan, Guangdong 528458, China;

Received Date: 22 September 2012
Available Online: 27 November 2012
Fund Project: 2012年广东省大学生创新创业训练计划(No.1057312013)资助项目。 (No.1057312013)

The interactions of the Ru(Ⅱ) complex, [Ru(phen)₂(Hecip)]₂₊ (phen=1,10-phenanthroline, Hecip=N-ethyl-4-([1,10]-phenanthroline[5,6-f]imidazol-2-yl)carbazole), with calf thymus DNA (CT DNA) were studied by using absorption spectroscopy, binding stoichiometry, viscosity measurement and photoactivated cleavage. A tight 2:1 complex is formed by the Ru(Ⅱ) polypyridyl complex and CT DNA with a binding constant exceeding 10⁵ mol^-1·L and with a binding mode of intercalation. Furthermore, the complex exhibits efficient DNA cleavage activity on UV (365 nm) irradiation via a mechanistic pathway involving formation of singlet oxygen as the reactive species. On the other hand, the cytotoxic activity of the complex was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) method. The complex shows prominent anticancer activity against selected tumor cell lines with IC₅₀ values lower than those of cisplatin. Further flow cytometry experiments show that the cytotoxic Ru(Ⅱ) complex can cause cell cycle arrest in the S phase.
- ruthenium complex;cytotoxicity;DNA-binding;cell cycle arrest
1. [1]
  [1] Greguric I, Aldrich-Wright J R, Collins J G. J. Am. Chem. Soc., 1997,119:3621-3622
2. [2]
  [2] Nair R B, Teng E S, Kirkland S L, et al. Inorg. Chem., 1998, 37:139-141
3. [3]
  [3] Erkkila K E, Odom D T, Barton J K. Chem. Rev., 1999,99: 2777-2796
4. [4]
  [4] Friedman A E, Chambron J C, Sauvage J P, et al. J. Am. Chem. Soc., 2004,126:8630-8631
5. [5]
  [5] Zeglis B M, Barton J K. J. Am. Chem. Soc., 2006,128:5654-5655
6. [6]
  [6] Liu Y, Hammitt R, Lutterman D A, et al. Inorg. Chem., 2007, 46:6011-6021
7. [7]
  [7] Cosgrave L, Devocelle M, Forster R J, et al. Chem. Commun., 2010,46:103-105
8. [8]
  [8] Tan C P, Wu S H, Lai S S, et al. Dalton Trans., 2011,40: 8611-8621
9. [9]
  [9] Tan L F, Shen J L, Liu J, et al. Dalton Trans., 2012,41:4575-4587
10. [10]
  [10] Liu Y J, Li Z Z, Liang Z H, et al. DNA Cell Biol., 2011,30: 839-848
11. [11]
  [11] Tan C P, Lai S S, Wu S H, et al. J. Med. Chem., 2010,53: 7613-7624
12. [12]
  [12] Schatzschneider U, Niesel J, Ott I, et al. ChemMedChem, 2008,3:1104-1109
13. [13]
  [13] Van Dijken A, Bastiaansen J J A M, Kiggen N M M, et al. J. Am. Chem. Soc., 2004,126:7718-7727
14. [14]
  [14] Grabowski Z R, Rotkiewicz K. Chem. Rev., 2003,103:3899-4032
15. [15]
  [15] Zhang Y, Wang L, Wada T, et al. Macromol. Chem. Phys., 1996,197:1877-1888
16. [16]
  [16] Wagner J, Pielichowski J, Hinsch A, et al. Synth. Met., 2004,146:159-165
17. [17]
  [17] Xin H, Sun M, Wang K Z, et al. Chem. Phys. Lett., 2004, 388:55-57
18. [18]
  [18] Liu F R, Wang K Z, Bai G Y, et al. Inorg. Chem., 2004,43: 1799-1806
19. [19]
  [19] Lü Y Y, Gao L H, Han M J, et al. Eur. J. Inorg. Chem., 2006,430:430-436
20. [20]
  [20] Xu L, Liu P X, Liao G L, et al. Aust. J. Chem., 2010,63:1-9
21. [21]
  [21] Marmur J A. J. Mol. Biol., 1961,3:208-218
22. [22]
  [22] Reichmann M E, Rice S A, Thomas C A, et al. J. Am. Chem. Soc., 1954,76:3047-3053
23. [23]
  [23] Wolf A, Shimer Jr G H, Meehan T. Biochemistry, 1987,26: 6392-6396
24. [24]
  [24] Chaires J B, Dattagupta N, Crothers D M. Biochemistry, 1982,21:3933-3940
25. [25]
  [25] Cohen G, Eisenberg H. Biopolymers, 1969,8:45-55
26. [26]
  [26] Job P. Ann. Chim., 1928,9:113-203
27. [27]
  [27] Mosmann T. J. Immunol. Methods., 1983,65:55-63
28. [28]
  [28] Tan L F, Song F C, Zou X Q, et al. DNA Cell Biol., 2011, 30:277-285
29. [29]
  [29] Pyle A M, Rehmann J P, Meshoyrer R, et al. J. Am. Chem. Soc., 1989,111:3051-3058
30. [30]
  [30] Han M J, Duan Z M, Wang K Z, et al. J. Phys. Chem. C., 2007,111:16577-16585
31. [31]
  [31] Friedman A E, Chambron J C, Sauvage J P, et al. J. Am. Chem. Soc., 1990,112:4960-4962
32. [32]
  [32] Tselepi-Kalouli E, Katsaros N. J. Inorg. Biochem., 1989,37: 271-282
33. [33]
  [33] Liu J G, Zhang Q L, Shi X F, et al. Inorg. Chem., 2001,40: 5045-5050
34. [34]
  [34] Satyanarayana S, Dabrowiak J C, Chaires J B. Biochemistry, 1992,31:9319-9324
35. [35]
  [35] Satyanaryana S, Daborusak J C, Chaires J B. Biochem., 1993,32:2573-2584
36. [36]
  [36] Cheng C C, Rokita S E, Burrows C J. Angew. Chem. Int. Ed. Engl., 1993,32:277-278
37. [37]
  [37] Lesko S A, Lorentzen R J, Ts'o P O. Biochemistry, 1980,19: 3023-3028
38. [38]
  [38] Nilsson R, Merkel P B, Kearns D R. Photochem. Photobiol., 1972,16:117-124
39. [39]
  [39] Patra A K, Nethaji M, Chakravarty A R. J. Inorg. Biochem., 2007,101:233-244
40. [40]
  [40] Deshpande M S, Kumbhar A A, Kumbhar A S, et al. Bioconjugate Chem., 2009,20:447-459
41. [41]
  [41] Gao F, Chao H, Ji L N. Chem. Biodivers., 2008,5:1962-1979
42. [42]
  [42] Yu H J, Chen Y, Yu L, et al. Eur. J. Med. Chem., 2012,55: 146-154
43. [43]
  [43] Karna P, Sharp S M, Yates C, et al. Mol. Cancer., 2009,8: 93
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