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Citation: YUAN Lian, LIU Yu-Jiao, HE Huan, JIANG Feng-Lei, LI Hui-Rong, LIU Yi. Microcalorimetric Analysis of Isolated Rat Liver Mitochondrial Metabolism under Different Conditions[J]. Acta Physico-Chimica Sinica, ;2018, 34(1): 73-80. doi: 10.3866/PKU.WHXB201707043 shu

Microcalorimetric Analysis of Isolated Rat Liver Mitochondrial Metabolism under Different Conditions

YUAN Lian ^1, ,
LIU Yu-Jiao ¹ ,
HE Huan ¹ ,
JIANG Feng-Lei ¹ ,
LI Hui-Rong ² ,
LIU Yi ¹

1.
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China;
2.
College of Life Science and Chemistry, Wuhan Donghu University, Wuhan 430212, P. R. China

Received Date: 13 June 2017
Revised Date: 19 June 2017

Fund Project: The project was supported by the National Natural Science Foundation of China (21673166) and Natural Science Foundation of Hubei Province,China (2015CFC892).

Isolated rat liver mitochondria were proposed as a model to monitor real-time heat metabolism.A high-throughput and sensitive thermal activity monitor III (TAM III) was used to detect the P-t curves of mitochondria under different conditions,including different mitochondrial concentrations,different substrates,different buffers,respiratory inhibitors,Ca²⁺,and CsA.We determined the thermokinetic parameters through calculation.The results showed that:(1) higher concentration of mitochondria led to faster energetic metabolism;(2) when succinate was the direct respiratory substrate,it promoted mitochondrial metabolism,in contrast to the condition when an indirect substrate,pyruvate,was used;(3) high concentration of Ca²⁺(2.5 mmol·L^-1) stimulated mitochondrial metabolism,however CsA,an inhibitor of mitochondrial permeability transition pores,could not reverse the Ca²⁺-induced mitochondrial alteration;(4) mitochondria in various buffers displayed different rates of heat metabolism,because of the different composition of the buffers;(5) mitochondrial metabolism was inhibited by respiratory inhibitors,especially NaN₃,which is an inhibitor of Complex IV and which completely stopped the mitochondrial heat release.
- Mitochondria,
- Microcalorimetry,
- Metabolic rate,
- Respiratory inhibitor,
- Mitochondrial permeability transition
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