Citation: FAN Zhi-Bo, GONG Xiao-Qing, LU Dan-Feng, GAO Ran, QI Zhi-Mei. Benzo[a]pyrene Sensing Properties of Surface Plasmon Resonance Imaging Sensor Based on the Hue Algorithm[J]. Acta Physico-Chimica Sinica, ;2017, 33(5): 1001-1009. doi: 10.3866/PKU.WHXB201701131 shu

Benzo[a]pyrene Sensing Properties of Surface Plasmon Resonance Imaging Sensor Based on the Hue Algorithm

FAN Zhi-Bo ^1,3, ,
GONG Xiao-Qing ^1,3 ,
LU Dan-Feng ¹ ,
GAO Ran ¹ ,
QI Zhi-Mei ^1,2

1.
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, P. R. China;
2.
State Key Laboratory of NBC Protection for Civilian, Beijing 102205, P. R. China;
3.
University of Chinese Academy of Sciences, Beijing 100049, P. R. China

Received Date: 1 December 2016
Revised Date: 13 January 2017

Fund Project: The project was supported by the National Key Basic Research Program of China (973) (2015CB352100), National Natural Science Foundation of China (61377064, 61675203) and Research Equipment Development Project of Chinese Academy of Sciences (YZ201508).

A colorful surface plasmon resonance imaging sensor for the in-situ detection of benzopyrene (BaP) in water is presented in this paper. The sensor can provide intuitive image information and can also quantitatively analyze the concentration and adsorption/desorption processes of the analyte by combining the hue algorithm. Both the resonance wavelengths and resonance images for a bare gold film chip were obtained at different incident angles using a home-made surface plasmon resonance (SPR) sensor that possesses wavelengthinterrogating and imaging capabilities. The relationship between the resonance wavelength and the average hue of the color image was established based on the hue algorithm. From this relationship, the initial resonance wavelength at which the SPR sensor can provide optimal hue sensitivity was derived, which was ~650 nm. Polytetrafluoroethylene (PTFE)-coated SPR sensor chips were prepared for the in-situ rapid detection of BaP in water based on the reversible enrichment of BaP molecules in the PTFE film. The results showed that: (1) the average hue of the SPR color image decreases linearly as BaP concentration increases from 20 to 100 nmol·L^-1; (2) both the response time and recovery times of the SPR sensor for 100 nmol·L^-1 BaP are 7 and 5 s, respectively; (3) since the thickness of the PTFE filmis greater than the penetration depth of the surface plasmon field, the BaP detection is not affected by the refractive index of the solution sample; and (4) in the case of a non-uniform PTFE film, the sensor allows to determine the hue sensitivities for equal-thickness microscale areas of the sensing film. The experimental results show that this type of colorful SPR imaging sensor has widespread applicability for chemical and biological detection.
- Color surface plasmon resonance imaging,
- Hue,
- Benzo[a]pyrene,
- PTFE sensing film,
- In-situ detection
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