Citation: ZHOU Qian, YU Jian-Qiang, ZHAO Li-Bo, LI De-Sheng, WU Kui, ZHU Jian-Hua, YUAN Jing-He, FANG Xiao-Hong. Super-Resolution Optical Subtraction Microscopy Using Optical Scattering Imaging[J]. Acta Physico-Chimica Sinica, ;2016, 32(5): 1123-1128. doi: 10.3866/PKU.WHXB201603234 shu

Super-Resolution Optical Subtraction Microscopy Using Optical Scattering Imaging

ZHOU Qian ^1,2,3 ,
YU Jian-Qiang ⁴ ,
ZHAO Li-Bo ³ ,
LI De-Sheng ⁵ ,
WU Kui ⁶ ,
ZHU Jian-Hua ^1,2 ,
YUAN Jing-He ^3,, ,
FANG Xiao-Hong ³

1.
1 Department of Physics, Sichuan University, Chengdu 610064, P. R. China;
2.
2 Key Laboratory of High Energy Density Physics and Technology of Ministry of Education, Chengdu 610064, P. R. China;
3.
3 Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China;
4.
4 College of Science, North China University of Technology, Beijing 100144, P. R. China;
5.
5 State Key Laboratory of Polymer Physics and Chemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China;
6.
6 Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China

Corresponding author: YUAN Jing-He,
Received Date: 25 February 2016
Available Online: 22 March 2016
Fund Project: 国家重点基础研究发展规划项目(973)(2013CB933701) (973)(2013CB933701)国家自然科学基金(21127901,91413119) (21127901,91413119)

The existing form of super-resolution microscopy based on specific fluorescent tagging is unable to obtain super-resolution images of non-fluorescent samples. Hence, we have developed optical subtraction microscopy for obtaining super-resolution imaging in such cases. This method is based on image subtraction between the two optical scattering images from general confocal excitation and doughnut-shaped excitation, respectively. Unlike super-resolution fluorescence microscopy, subtraction microscopy requires no preprocessing of the sample, and the excitation power can be kept low to avoid sample damage. The non-fluorescent imaging of gold nanobeads and polymer nanofibers has been realized to demonstrate the feasibility of super-resolution subtraction microscopy. The lateral resolution decreases to 215 nm (0.33λ, 1λ = 650 nm) in subtraction imaging, and greater imaging detail of the sample is achieved via optical scattering.
- Super-resolution microscopy,
- Subtraction imaging,
- Non-fluorescence imaging,
- Optical scattering imaging
1. [1]
  (1) Rust, M. J.; Bates, M.; Zhuang, X.W. Nature Methods 2006, 3, 793. doi: 10.1038/nmeth929
2. [2]
  (2) Betzig, E.; Patterson, G. H.; Sougrat, R.; Lindwasser, O.W.; Olenych, S.; Bonifacino, J. S.; Davidson, M.W.; Lippincott-Schwartz, J.; Hess, H. F. Science 2006, 313, 1642. doi: 10.1126/science.1127344
3. [3]
  (3) Klar, T. A.; Jakobs, S.; Dyba, M.; Egner, A.; Hell, S.W. Proceedings of the National Academy of Sciences of the United States of America 2000, 97, 8206. doi: 10.1073/pnas.97.15.8206
4. [4]
  (4) Hell, S.W. Science 2007, 316, 1153. doi: 10.1126/science.1137395
5. [5]
  (5) Wang, P.; Slipchenko, M. N.; Mitchell, J.; Yang, C.; Potma, E. O.; Xu, X. F.; Cheng, J. X. Nat. Photonics 2013, 7, 450. doi: 10.1038/NPHOTON.2013.97
6. [6]
  (6) Ye, H.; Qiu, C.W.; Huang, K.; Teng, J.; Luk'Yanchuk, B.; Yeo, S. P. Laser Phys. Lett. 2013, 10, 64. doi: 10.1088/1612-2011/10/6/065004
7. [7]
  (7) Haeberle, O.; Simon, B. Opt. Commun. 2009, 282, 3657. doi: 10.1016/j.optcom.2009.06.025
8. [8]
  (8) Dehez, H.; Piche, M.; De Koninck, Y. Opt. Express 2013, 21, 15912. doi: 10.1364/OE.21.015912
9. [9]
  (9) Segawa, S.; Kozawa, Y.; Sato, S. Opt. Lett. 2014, 39, 4529. doi: 10.1364/OL.39.004529
10. [10]
  (10) Kuang, C. F.; Li, S.; Liu, W.; Hao, X.; Gu, Z. T.; Wang, Y. F.; Ge, J. H.; Li, H. F.; Liu, X. Sci. Rep-UK 2013, 3, 6. doi: 10.1038/srep01441
11. [11]
  (11) Wolf, E. Proceedings of the Royal Society of London Series a-Mathematical and Physical Sciences 1959, 253, 349. doi: 10.1098/rspa.1959.0199
12. [12]
  (12) Richards, B.; Wolf, E. Proceedings of the Royal Society of London Series a-Mathematical and Physical Sciences 1959, 253, 358. doi: 10.1098/rspa.1959.0200
13. [13]
  (13) Klar, T. A.; Engel, E.; Hell, S.W. Phys. Rev. E 2001, 64, 9. doi: 10.1103/PhysRevE.64.066613
14. [14]
  (14) Yu, J. Q.; Yuan, J. H.; Zhang, X. J.; Liu, J. L.; Fang, X. H. Chinese Science Bulletin 2013, 58, 4045. doi: 10.1007/s11434-013-6011-z
1. [1]
  Siyi ZHONG , Xiaowen LIN , Jiaxin LIU , Ruyi WANG , Tao LIANG , Zhengfeng DENG , Ao ZHONG , Cuiping HAN . Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1483-1490. doi: 10.11862/CJIC.20240093
2. [2]
  Jinlong YAN , Weina WU , Yuan WANG . A simple Schiff base probe for the fluorescent turn-on detection of hypochlorite and its biological imaging application. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1653-1660. doi: 10.11862/CJIC.20240154
3. [3]
  Liang TANG , Jingfei NI , Kang XIAO , Xiangmei LIU . Synthesis and X-ray imaging application of lanthanide-organic complex-based scintillators. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1892-1902. doi: 10.11862/CJIC.20240139
4. [4]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
5. [5]
  Donghui PAN , Yuping XU , Xinyu WANG , Lizhen WANG , Junjie YAN , Dongjian SHI , Min YANG , Mingqing CHEN . Preparation and in vivo tracing of ⁶⁸Ga-labeled PM_2.5 mimetic particles for positron emission tomography imaging. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 669-676. doi: 10.11862/CJIC.20230468
6. [6]
  Qiuyu Xiang , Chunhua Qu , Guang Xu , Yafei Yang , Yue Xia . A Journey beyond “Alum”. University Chemistry, 2024, 39(11): 189-195. doi: 10.12461/PKU.DXHX202404094
7. [7]
  Wei Peng , Baoying Wen , Huamin Li , Yiru Wang , Jianfeng Li . Exploration and Practice on Raman Scattering Spectroscopy Experimental Teaching. University Chemistry, 2024, 39(8): 230-240. doi: 10.3866/PKU.DXHX202312062
8. [8]
  Yingxian Wang , Tianye Su , Limiao Shen , Jinping Gao , Qinghe Wu . Introduction of Chinese Lacquer from the Perspective of Chemistry: Popularizing Chemistry in Lacquer and Inherit Lacquer Art. University Chemistry, 2024, 39(5): 371-379. doi: 10.3866/PKU.DXHX202312015
9. [9]
  Bing Sun . Practice of Ideological and Political Education in Physical Chemistry Courses for Non-Chemistry Majors. University Chemistry, 2024, 39(8): 28-35. doi: 10.3866/PKU.DXHX202311080
10. [10]
  Yan ZHAO , Xiaokang JIANG , Zhonghui LI , Jiaxu WANG , Hengwei ZHOU , Hai GUO . Preparation and fluorescence properties of Eu³⁺-doped CaLaGaO₄ red-emitting phosphors. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1861-1868. doi: 10.11862/CJIC.20240242
11. [11]
  Xinyu Liu , Weiran Hu , Zhengkai Li , Wei Ji , Xiao Ni . Algin Lab: Surging Luminescent Sea. University Chemistry, 2024, 39(5): 396-404. doi: 10.3866/PKU.DXHX202312021
12. [12]
  Xin Lv , Hongxing Zhang , Kaibo Duan , Wenhui Dai , Zhihui Wen , Wei Guo , Junsheng Hao . Lighting the Way Against Cancer: Photodynamic Therapy. University Chemistry, 2024, 39(5): 70-79. doi: 10.3866/PKU.DXHX202309090
13. [13]
  Chun-Lin Sun , Yaole Jiang , Yu Chen , Rongjing Guo , Yongwen Shen , Xinping Hui , Baoxin Zhang , Xiaobo Pan . Construction, Performance Testing, and Practical Applications of a Home-Made Open Fluorescence Spectrometer. University Chemistry, 2024, 39(5): 287-295. doi: 10.3866/PKU.DXHX202311096
14. [14]
  Jianjun Liu , Xue Yang , Chi Zhang , Xueyu Zhao , Zhiwei Zhang , Yongmei Chen , Qinghong Xu , Shao Jin . Preparation and Fluorescence Characterization of CdTe Semiconductor Quantum Dots. University Chemistry, 2024, 39(7): 307-315. doi: 10.3866/PKU.DXHX202311031
15. [15]
  Zishuo Yi , Peng Liu , Yan Xu . Fluorescent “Chameleon”: A Popular Science Experiment Based on Dynamic Luminescence. University Chemistry, 2024, 39(9): 304-310. doi: 10.12461/PKU.DXHX202311079
16. [16]
  Jin Tong , Shuyan Yu . Crystal Engineering for Supramolecular Chirality. University Chemistry, 2024, 39(3): 86-93. doi: 10.3866/PKU.DXHX202308113
17. [17]
  Laiying Zhang , Yinghuan Wu , Yazi Yu , Yecheng Xu , Haojie Zhang , Weitai Wu . Innovation and Practice of Polymer Chemistry Experiment Teaching for Non-Polymer Major Students of Chemistry: Taking the Synthesis, Solution Property, Optical Performance and Application of Thermo-Sensitive Polymers as an Example. University Chemistry, 2024, 39(4): 213-220. doi: 10.3866/PKU.DXHX202310126
18. [18]
  Peiran ZHAO , Yuqian LIU , Cheng HE , Chunying DUAN . A functionalized Eu³⁺ metal-organic framework for selective fluorescent detection of pyrene. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 713-724. doi: 10.11862/CJIC.20230355
19. [19]
  Jiakun BAI , Ting XU , Lu ZHANG , Jiang PENG , Yuqiang LI , Junhui JIA . A red-emitting fluorescent probe with a large Stokes shift for selective detection of hypochlorous acid. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1095-1104. doi: 10.11862/CJIC.20240002
20. [20]
  Lin Song , Dourong Wang , Biao Zhang . Innovative Experimental Design and Research on Preparing Flexible Perovskite Fluorescent Gels Using 3D Printing. University Chemistry, 2024, 39(7): 337-344. doi: 10.3866/PKU.DXHX202310107

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(786)
HTML views(76)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142