Citation: GUO Qing-Lian, HE Huan, PAN Ling-Li, LIU Yi. Thermodynamics of the Interaction of BCBP with Bovine Serum Albumin[J]. Acta Physico-Chimica Sinica, ;2016, 32(6): 1383-1390. doi: 10.3866/PKU.WHXB201603093 shu

Thermodynamics of the Interaction of BCBP with Bovine Serum Albumin

GUO Qing-Lian ^1, ,
HE Huan ² ,
PAN Ling-Li ³ ,
LIU Yi ²

1.
Department of Clinical Laboratory, Zhongnan Hospital, Wuhan University, Wuhan 430071, P. R. China;
2.
College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China;
3.
Department of Emergency, Huangshi Central Hospital (Affiliated Hospital of Hubei Polytechnic University), Edong Healthcare Group, Huangshi 435002, Hubei Province, P. R. China

Received Date: 14 January 2016
Revised Date: 8 March 2016

Fund Project: The project was supported by the Key Project of Health and Family Planning Commission of Hubei Province, China (WJ2015MB097) and Wuhan Yellow Crane Talents Program for Science and Technology, China (2014[10]).

Celecoxib derivatives are widely used, non-steroidal, anti-inflammatory drugs for the treatment of acute or chronic inflammation. Under simulated physiological conditions, we used fluorescence and ultraviolet absorption spectroscopy, circular dichroism, and methods of molecular simulation to study the thermodynamics of the interaction between the celecoxib derivative 1-benzenesulfonamides-3-carboxyl-5-phenyl pyrazole (BCBP) and bovine serum albumin (BSA). The fluorescence quenching of BSA by BCBP was a static process, which was confirmed by the UV-Vis absorption spectra. The calculated enthalpy (ΔH) and entropy (ΔS) changes implied that hydrogen bonds and van der Waals forces played a predominant role in the binding process. The circular dichroism demonstrated that the secondary structure of BSA changed after its interaction with BCBP, causing the α-helix content to decrease, accompanied by an increase in an unordered structure. Molecular docking results confirmed the experimental results.
- Celecoxib derivative,
- Bovine serum albumin,
- Interaction,
- Thermodynamics parameter
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