Citation: HE Wen-Ying, YAO Xiao-Jun, HUA Ying-Jie, HUANG Guo-Lei, WU Xiu-Li, LI Xiao-Bao, HAN Chang-Ri, SONG Xiao-Ping. Effect of Kolavenic Acid on the Structure of Human Serum Albumin[J]. Acta Physico-Chimica Sinica, ;2014, 30(11): 2142-2148. doi: 10.3866/PKU.WHXB201409253 shu

Effect of Kolavenic Acid on the Structure of Human Serum Albumin

1.
1. College of Chemical and Chemical Engineering, Hainan Normal University, Haikou 571158, P. R. China;
2. College of Chemical and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China;
3. Key Laboratory of Tropical Medicimal Plant Chemistry of Ministry of Education, Hainan Normal University, Haikou 571158, P. R. China

Received Date: 1 July 2014
Available Online: 25 September 2014
Fund Project: 国家国际科技合作专项(2014DFA40850) (2014DFA40850)国家自然科学基金(81160391)资助项目 (81160391)

The effect of Kolavenic acid (KA), an active component isolated from the genus Polyalthia, on the structure of human serum albumin (HSA) was investigated by fluorescence polarization, synchronous fluorescence, three-dimensional (3D) fluorescence, and absorption spectroscopy in combination with molecular modeling techniques under physiological conditions. The synchronous and absorption fluorescence spectra confirmed that KA has an effect on the microenvironment around HSA in aqueous solution. The two-dimensional (2D) and 3D fluorescence spectra showed that KA could quench the intrinsic fluorescence of HSA and make its conformation change. Fluorescence polarization measurements provided useful information on the relaxation time and aggregation behavior of the complex formed between HSA and KA, and indicated that the presence of KA caused changes in the fluidity and microviscosity of HSA. The binding constants and thermodynamic parameters for KA-HSA systems were obtained under different temperatures (298, 308, and 318 K). Molecular docking showed that the KAmoiety bound to the hydrophobic cavity of HSA, and there were three hydrogenbonding interactions between KAand the Lys195 andAsp451 residues. Fluorescent displacement measurements confirmed that KA bound to HSA at site Ⅱ. In addition, the binding mechanism of KA and HSA was revealed by the physicochemical parameters at the molecular level. The results showed that the interaction between KA and HSA was strong, indicating that KA may be stored and transferred by serum albumin.
- Kolavenic acid,
- Human serum albumin,
- Molecular modeling,
- Fluorescence spectroscopy,
- UV-visible absorption spectroscopy,
- Interaction
1. [1]
  
  (1) Li, X. B. Studies on Chemical Constituents and Pharmacogical Activities of the Roots of Polyalthia laui. Master Dissertation, Hainan Normal University, Haikou, 2012. [李小宝. 海南暗罗根化学成分及其药理活性研究[M]. 海口: 海南师范大学, 2012.]
2. [2]
  (2) http://www.med126.com/pharm/2010/20100123233057_164906.shtml, 2010 (accessed July 1, 2014).
3. [3]
  (3) Liu, Y.; Long, M.; Xie, M. X. Acta Phys. -Chim. Sin. 2013, 29, 2647. [刘媛, 龙梅, 谢孟峡. 物理化学学报, 2013, 29, 2647.] doi: 10.3866/PKU.WHXB201310311
4. [4]
  (4) Ning, A. M.; Meng, L.; Zhao, Z. L.; Zheng, X. F.;Wan, X. S. Acta Phys. -Chim. Sin. 2013, 29, 2639. [宁爱民, 孟磊, 赵仲麟, 郑先福, 宛新生. 物理化学学报, 2013, 29, 2639.] doi: 10.3866/PKU.WHXB201310281
5. [5]
  (5) wda, J. I.; Nandibewoor, S. T. Spectrochimica Acta Part A 2014, 124, 397. doi: 10.1016/j.saa.2014.01.028
6. [6]
  (6) Tunc, S.; Duman, O.; Soylu, I.; Bozo?lan, B. J. Lumin. 2014, 151, 22. doi: 10.1016/j.jlumin.2014.02.004
7. [7]
  (7) Tao,W. S.; Li,W.; Jiang, Y. M. The Basic of Protein Molecules, 2nd ed.; Higher Education Press: Beijing, 1995; pp 350-355. [陶慰孙, 李惟, 姜涌明. 蛋白质分子基础. 第二版. 北京: 高等教育出版社, 1995: 350-355.]
8. [8]
  (8) Yang, P.; Gao, F. Principles of Bioinorganic Chemistry; Science Press: Beijing, 2002; pp 322-342. [杨频, 高飞. 生物无机化学原理. 北京: 科学出版社, 2002: 322-342.]
9. [9]
  (9) Zhu, B.; Du, X. L.; Li, R. C.;Wang, K.; Jin, J.;Wang, B. C. Chem. J. Chin. Univ. 2001, 22, 26. [朱兵, 杜秀莲, 李荣昌, 王夔, 金坚, 王博诚. 高等学校化学学报, 2001, 22, 26.]
10. [10]
  (10) Liu, G. Q.; Yue, H. Q.; Zhang, Y. Mod. Instruments Med. Treat. 2004, 6, 33. [刘桂琴, 岳慧琴, 张勇. 现代仪器与医疗, 2004, 6, 33.]
11. [11]
  (11) Lakowicz, J. R. Principles of Fluorescence Spectroscopy; Plenum: New York, 1983; pp 341-379.
12. [12]
  (12) SYBYL Software, Version 6.9; Tripos Associates Inc.: St. Louis, MO, USA, 2002.
13. [13]
  (13) Morris, G. M.; odsell, D. S.; Huey, R.; Olson, A. J. J. Comput-Aided. Mol. Des. 1996, 10, 293. doi: 10.1007/BF00124499
14. [14]
  (14) Morris, G. M.; odsell, D. S.; Halliday, R. S.; Huey, R.; Hart, W. E.; Belew, R. K.; Olson, A. J. J. Comput. Chem. 1998, 19, 1639.
15. [15]
  (15) Carter, D. C.; He, X. M.; Munson, S. H.; Twigg, P. D.; Gernert, K. M.; Broom, M. B.; Miller, T. Y. Science 1989, 244, 1195. doi: 10.1126/science.2727704
16. [16]
  (16) Wolfbeis, O. S.; Leiner, M.; Hochmuth, P.; Geiger, H.; Bunsenges, B. Bunsenges. Phys. Chem. 1984, 88, 759. doi: 10.1002/bbpc.19840880817
17. [17]
  (17) Valeur, B. Molecular Fluorescence: Principles and Applications;Wiley-VCH Verlag GmbH. ISBNs: 3-527-29919-X (Hardcover); 3-527-60024-8 (Electronic). 2001; pp 56-68.
18. [18]
  (18) Ross, P. D.; Subramanian, S. Biochemistry 1981, 20, 3096. doi: 10.1021/bi00514a017
19. [19]
  (19) Hill, J. J.; Royer, C. A. Methods in Enzymology; Academic Press: San Die , 1997; pp 390-416.
20. [20]
  (20) Kido, C.; Murano, S.; Tsuruoka, M. Gene 2000, 259, 123. doi: 10.1016/S0378-1119(00)00457-1
21. [21]
  (21) Yang, Y.; Ye, X. L.; Li, X. G. Negative 2009, 17, 1627. [杨勇, 叶小利, 李学刚. 医学争鸣, 2009, 17, 1627.](22) Sudlow, G.; Birkett, D. J.;Wade, D. N. Mol. Pharmacol. 1976, 12, 1052.(23) Khan, A. M.; Muzammil, S.; Musarrat, J. Int. J. Biol. Macromol. 2002, 30, 243. doi: 10.1016/S0141-8130(02)00038-7
22. [22]
  (24) Mohammed, H. R.; Toru, M.; Tomoko, O.; Keishi, Y.; Masaki, O. Biochem. Pharm. 1993, 46, 1721. doi: 10.1016/0006-2952(93)90576-I
23. [23]
  (25) Wuhan University. Analytical Chemistry; People Press: Beijing, 1978; pp 131-132. [武汉大学. 分析化学. 北京: 人民出版社, 1978: 131-132.]
24. [24]
  (26) Gao, H.; Lei, L. D.; Liu, J. Q.; Kong, Q.; Chen, X. G.; Hu, Z. D. J. Photochem. Photobiol. A 2004, 167, 213. doi: 10.1016/j.jphotochem.2004.05.017
1. [1]
  Yi Li , Zhaoxiang Cao , Peng Liu , Xia Wu , Dongju Zhang . Revealing the Coloration and Color Change Mechanisms of the Eriochrome Black T Indicator through Computational Chemistry and UV-Visible Absorption Spectroscopy. University Chemistry, 2025, 40(3): 132-139. doi: 10.12461/PKU.DXHX202405154
2. [2]
  Feng Zheng , Ruxun Yuan , Xiaogang Wang . “Research-Oriented” Comprehensive Experimental Design in Polymer Chemistry: the Case of Polyimide Aerogels. University Chemistry, 2024, 39(10): 210-218. doi: 10.12461/PKU.DXHX202404027
3. [3]
  Jiaxun Wu , Mingde Li , Li Dang . The R eaction of Metal Selenium Complexes with Olefins as a Tutorial Case Study for Analyzing Molecular Orbital Interaction Modes. University Chemistry, 2025, 40(3): 108-115. doi: 10.12461/PKU.DXHX202405098
4. [4]
  Chun-Lin Sun , Yaole Jiang , Yu Chen , Rongjing Guo , Yongwen Shen , Xinping Hui , Baoxin Zhang , Xiaobo Pan . Construction, Performance Testing, and Practical Applications of a Home-Made Open Fluorescence Spectrometer. University Chemistry, 2024, 39(5): 287-295. doi: 10.3866/PKU.DXHX202311096
5. [5]
  Jizhou Liu , Chenbin Ai , Chenrui Hu , Bei Cheng , Jianjun Zhang . 六氯锡酸铵促进钙钛矿太阳能电池界面电子转移及其飞秒瞬态吸收光谱研究. Acta Physico-Chimica Sinica, 2024, 40(11): 2402006-. doi: 10.3866/PKU.WHXB202402006
6. [6]
  Mengyao Shi , Kangle Su , Qingming Lu , Bin Zhang , Xiaowen Xu . Determination of Potassium Content in Tobacco Stem Ash by Flame Atomic Absorption Spectroscopy. University Chemistry, 2024, 39(10): 255-260. doi: 10.12461/PKU.DXHX202404105
7. [7]
  Huiying Xu , Minghui Liang , Zhi Zhou , Hui Gao , Wei Yi . Application of Quantum Chemistry Computation and Visual Analysis in Teaching of Weak Interactions. University Chemistry, 2025, 40(3): 199-205. doi: 10.12461/PKU.DXHX202407011
8. [8]
  Chuanming GUO , Kaiyang ZHANG , Yun WU , Rui YAO , Qiang ZHAO , Jinping LI , Guang LIU . Performance of MnO₂-0.39IrO_x composite oxides for water oxidation reaction in acidic media. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1135-1142. doi: 10.11862/CJIC.20230459
9. [9]
  Yaping Li , Sai An , Aiqing Cao , Shilong Li , Ming Lei . The Application of Molecular Simulation Software in Structural Chemistry Education: First-Principles Calculation of NiFe Layered Double Hydroxide. University Chemistry, 2025, 40(3): 160-170. doi: 10.12461/PKU.DXHX202405185
10. [10]
  Hailian Tang , Siyuan Chen , Qiaoyun Liu , Guoyi Bai , Botao Qiao , Fei Liu . Stabilized Rh/hydroxyapatite Catalyst for Furfuryl Alcohol Hydrogenation: Application of Oxidative Strong Metal-Support Interactions in Reducing Conditions. Acta Physico-Chimica Sinica, 2025, 41(4): 100036-. doi: 10.3866/PKU.WHXB202408004
11. [11]
  Zhuomin Zhang , Hanbing Huang , Liangqiu Lin , Jingsong Liu , Gongke Li . Course Construction of Instrumental Analysis Experiment: Surface-Enhanced Raman Spectroscopy for Rapid Detection of Edible Pigments. University Chemistry, 2024, 39(2): 133-139. doi: 10.3866/PKU.DXHX202308034
12. [12]
  Jingyi Chen , Fu Liu , Tiejun Zhu , Kui Cheng . Practice of Integrating Ideological and Political Education into Raman Spectroscopy Analysis Experiment Course. University Chemistry, 2024, 39(2): 140-146. doi: 10.3866/PKU.DXHX202310111
13. [13]
  Wei Peng , Baoying Wen , Huamin Li , Yiru Wang , Jianfeng Li . Exploration and Practice on Raman Scattering Spectroscopy Experimental Teaching. University Chemistry, 2024, 39(8): 230-240. doi: 10.3866/PKU.DXHX202312062
14. [14]
  Zhaoyue Lü , Zhehao Chen , Yi Ni , Duanbin Luo , Xianfeng Hong . Multi-Level Teaching Design and Practice Exploration of Raman Spectroscopy Experiment. University Chemistry, 2024, 39(11): 304-312. doi: 10.12461/PKU.DXHX202402047
15. [15]
  Liang MA , Honghua ZHANG , Weilu ZHENG , Aoqi YOU , Zhiyong OUYANG , Junjiang CAO . Construction of highly ordered ZIF-8/Au nanocomposite structure arrays and application of surface-enhanced Raman spectroscopy. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1743-1754. doi: 10.11862/CJIC.20240075
16. [16]
  Wenliang Wang , Weina Wang , Lixia Feng , Nan Wei , Sufan Wang , Tian Sheng , Tao Zhou . Proof and Interpretation of Severe Spectroscopic Selection Rules. University Chemistry, 2025, 40(3): 415-424. doi: 10.12461/PKU.DXHX202408063
17. [17]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
18. [18]
  Tianlong Zhang , Jiajun Zhou , Hongsheng Tang , Xiaohui Ning , Yan Li , Hua Li . Virtual Simulation Experiment for Laser-Induced Breakdown Spectroscopy (LIBS) Analysis. University Chemistry, 2024, 39(6): 295-302. doi: 10.3866/PKU.DXHX202312049
19. [19]
  Kaifu Zhang , Shan Gao , Bin Yang . Application of Theoretical Calculation with Fun Practice in Raman Spectroscopy Experimental Teaching. University Chemistry, 2025, 40(3): 62-67. doi: 10.12461/PKU.DXHX202404045
20. [20]
  Pingping Zhu , Yongjun Xie , Yuanping Yi , Yu Huang , Qiang Zhou , Shiyan Xiao , Haiyang Yang , Pingsheng He . Excavation and Extraction of Ideological and Political Elements for the Virtual Simulation Experiments at Molecular Level: Taking the Project “the Simulation and Computation of Conformation, Morphology and Dimensions of Polymer Chains” as an Example. University Chemistry, 2024, 39(2): 83-88. doi: 10.3866/PKU.DXHX202309063

Get Citation

PDF

XML

Metrics

PDF Downloads(337)
Abstract views(775)
HTML views(26)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142