Citation: HU Li-Mei, LIN Cun-Guo, WANG Li, YUAN Shi-Ling. Lysozyme Protein Adsorbed on Antifouling Polymer Film Surface[J]. Acta Physico-Chimica Sinica, ;2014, 30(11): 2149-2156. doi: 10.3866/PKU.WHXB201409021 shu

Lysozyme Protein Adsorbed on Antifouling Polymer Film Surface

HU Li-Mei ^1,2 ,
LIN Cun-Guo ² ,
WANG Li ² ,
YUAN Shi-Ling ^1,

1.
1. Key Laboratory of Colloid and Interface Chemistry, Shandong University, Jinan 250100, P. R. China;
2. State Key Laboratory for Marine Corrosion and Protection, Luoyang Ship Material Research Institute LSMRI, Qingdao 266101, Shandong Province, P. R. China

Received Date: 31 July 2014
Available Online: 2 September 2014
Fund Project: 国家重点基础研究发展规划项目(973) (2014CB643305)资助 (973) (2014CB643305)

Molecular dynamics simulations were used to compare the adsorption behavior of lysozyme on two typical antifouling polymer materials: poly(ethylene) glycol (PEG) and poly(dimethylsiloxane) (PDMS). The influence of the surface properties of the polymer films on protein adsorption is discussed at the microscale. Based on the interactions, energy changes between the protein and polymer films, and dynamical behavior of the hydration molecules near the polymer film, the reasons why the PEG antifouling coating has a better antifouling effect than the PDMS surface were determined as follows. (1) The lower binding energy between the protein and the PEG coating than between the protein and the PDMS coating makes the protein adsorb weaker on the PEG coating than on the PDMS coating. (2) The protein would adsorb on the film surface when overcoming the energy barrier caused by the hydration layer. Molecular water adsorbs on the PEG surface stronger than on the PDMS surface, and is difficult to desorb. Therefore, the protein needs to overcome a higher energy barrier to adsorb to the PEG surface than to the PDMS surface, and thus it is more difficult for protein to absorb on the PEG surface than on the PDMS surface.
- Poly(ethylene) glycol,
- Poly(dimethylsiloxane),
- Antifouling material,
- Molecular dynamics
1. [1]
  
  (1) Ostuni, E.; Chapman, R. G.; Holmlin, R. K.; Takayama, S.; Whitesides, G. M. Langmuir 2001, 17, 5605. doi: 10.1021/la010384m
2. [2]
  (2) Shen, M. C.; Martinson, L.;Wagner, M. S.; Castner, D. G.; Ratner, B. D.; Horbett, T. A. J. Biomater. Sci. Polym. Ed. 2002, 13, 367. doi: 10.1163/156856202320253910
3. [3]
  (3) Chambers, L. D.; Stokes, K. R.;Walsh, F. C.;Wood, R. J. Surface and Coatings Technology 2006, 201 (6), 364.
4. [4]
  (4) Zheng, J.; Li, L.; Chen, S.; Jiang, S. Langmuir 2004, 20 (20), 8931. doi: 10.1021/la036345n
5. [5]
  (5) Cedervall, T.; Lynch, I.; Foy, M.; Berggård, T.; Donnelly, S. C.; Cagney, G.; Dawson, K. A. Angewandte Chemie International Edition 2007, 46 (30), 5754.
6. [6]
  (6) Aggarwal, P.; Hall, J. B.; McLeland, C. B.; Dobrovolskaia, M. A.; McNeil, S. E. Advanced Drug Delivery Reviews 2009, 61 (6), 428. doi: 10.1016/j.addr.2009.03.009
7. [7]
  (7) Kitano, H.; Sudo, K.; Ichikawa, K.; Ide, M.; Ishihara, K. The Journal of Physical Chemistry B 2000, 104 (47), 11425. doi: 10.1021/jp000429c
8. [8]
  (8) Zheng, H. R.;Wang, X.W.; Lin, X. H.; Geng, Q.; Chen, X.; Dai,W. X.;Wang, X. X. Acta Phys. -Chim. Sin. 2012, 28, 1764. [郑华荣, 王晓韡, 林霞晖, 耿强, 陈旬, 戴文新, 王绪绪. 物理化学学报, 2012, 28, 1764.] doi: 10.3866/PKU.WHXB201205112
9. [9]
  (9) Lüsse, S.; Arnold, K. Macromolecules 1996, 29 (12), 4251. doi: 10.1021/ma9508616
10. [10]
  (10) Wang, Y. Q.;Wang, T.; Su, Y. L.; Peng, F. B.;Wu, H.; Jiang, Z. Y. Langmuir 2005, 21 (25), 11856. doi: 10.1021/la052052d
11. [11]
  (11) Zhao,W.; Su, Y.; Li, C.; Shi, Q.; Ning, X.; Jiang, Z. Journal of Membrane Science 2008, 318 (1), 405.
12. [12]
  (12) Chen, H.; Yuan, L.; Song,W.;Wu, Z.; Li, D. Progress in Polymer Science 2008, 33 (11), 1059. doi: 10.1016/j.progpolymsci.2008.07.006
13. [13]
  (13) Michalkova, A.; Tulyani, S.; Beals, J.; Leszczynski, J. Journal of Molecular Modeling 2012, 18 (1), 239. doi: 10.1007/s00894-011-1058-8
14. [14]
  (14) Shen, J.W.;Wu, T.;Wang, Q.; Pan, H. H. Biomaterials 2008, 29 (5), 513. doi: 10.1016/j.biomaterials.2007.10.016
15. [15]
  (15) Bai, S.; Li, H.; Zhang, L. Acta Phys. -Chim. Sin. 2013, 29, 849. [白姝, 李浩, 张麟. 物理化学学报, 2013, 29, 849.] doi: 10.3866/PKU.WHXB201301182
16. [16]
  (16) Fang, Y. Y.; Hu, X. G.; Yu, L.; Li,W. B.; Lin, R. S. Acta Phys. -Chim. Sin. 2007, 23, 84. [方盈盈, 胡新根, 于丽, 李文兵, 林瑞森. 物理化学学报, 2007, 23, 84.] doi: 10.3866/PKU.WHXB20070117
17. [17]
  (17) Panos, M.; Sen, T. Z.; Ahunbay, M. G. Langmuir 2012, 28 (34), 12619. doi: 10.1021/la301546v
18. [18]
  (18) Schuler, L. D.; Daura, X.; Van Gunsteren,W. F. Journal of Computational Chemistry 2001, 22 (11), 1205.
19. [19]
  (19) Van der Spoel, D.; Lindahl, E.; Hess, B.; Groenhof, G.; Mark, A. E.; Berendsen, H. J. Journal of Computational Chemistry 2005, 26 (16), 1701.
20. [20]
  (20) Hess, B.; Kutzner, C.; Van der Spoel, D.; Lindahl, E. Journal of Chemical Theory and Computation 2008, 4 (3), 435. doi: 10.1021/ct700301q
21. [21]
  (21) Lindahl, E.; Hess, B.; Van der Spoel, D. Molecular Modeling Annual 2001, 7 (8), 306.
22. [22]
  (22) Essmann, U.; Perera, L.; Berkowitz, M. L.; Darden, T.; Lee, H.; Pedersen, L. G. The Journal of Chemical Physics 1995, 103 (19), 8577. doi: 10.1063/1.470117
23. [23]
  (23) Berendsen, H. J.; Postma, J. P. M.; van Gunsteren,W. F.; DiNola, A. R. H. J.; Haak, J. R. The Journal of Chemical Physics 1984, 81 (8), 3684. doi: 10.1063/1.448118
24. [24]
  (24) Hess, B.; Bekker, H.; Berendsen, H. J.; Fraaije, J. G. Journal of Computational Chemistry 1997, 18 (12), 1463.
25. [25]
  (25) Dismer, F.; Hubbuch, J. Journal of Chromatography A 2007, 1149 (2), 312. doi: 10.1016/j.chroma.2007.03.074
26. [26]
  (26) Hower, J. C.; He, Y.; Bernards, M. T.; Jiang, S. The Journal of Chemical Physics 2006, 125 (21), 214704. doi: 10.1063/1.2397681
27. [27]
  (27) Vanderah, D. J.; La, H.; Naff, J.; Silin, V.; Rubinson, K. A. Journal of the American Chemical Society 2004, 126 (42), 13639. doi: 10.1021/ja047744n
28. [28]
  (28) Shao, Q.; He, Y.; White, A. D.; Jiang, S. The Journal of Physical Chemistry B 2010, 114 (49), 16625. doi: 10.1021/jp107272n
29. [29]
  (29) He, Y.; Hower, J.; Chen, S.; Bernards, M. T.; Chang, Y.; Jiang, S. Langmuir 2008, 24 (18), 10358. doi: 10.1021/la8013046
30. [30]
  (30) He, Y.; Chang, Y.; Hower, J. C.; Zheng, J.; Chen, S.; Jiang, S. Physical Chemistry Chemical Physics 2008, 10 (36),5539. doi: 10.1039/b807129b
1. [1]
  Xinhao Yan , Guoliang Hu , Ruixi Chen , Hongyu Liu , Qizhi Yao , Jiao Li , Lingling Li . Polyethylene Glycol-Ammonium Sulfate-Nitroso R Salt System for the Separation of Cobalt (II). University Chemistry, 2024, 39(6): 287-294. doi: 10.3866/PKU.DXHX202310073
2. [2]
  Shule Liu . Application of SPC/E Water Model in Molecular Dynamics Teaching Experiments. University Chemistry, 2024, 39(4): 338-342. doi: 10.3866/PKU.DXHX202310029
3. [3]
  Yue Wu , Jun Li , Bo Zhang , Yan Yang , Haibo Li , Xian-Xi Zhang . Research on Kinetic and Thermodynamic Transformations of Organic-Inorganic Hybrid Materials for Fluorescent Anti-Counterfeiting Application information: Introducing a Comprehensive Chemistry Experiment. University Chemistry, 2024, 39(6): 390-399. doi: 10.3866/PKU.DXHX202403028
4. [4]
  Xiaochen Zhang , Fei Yu , Jie Ma . 多角度数理模拟在电容去离子中的前沿应用. Acta Physico-Chimica Sinica, 2024, 40(11): 2311026-. doi: 10.3866/PKU.WHXB202311026
5. [5]
  Yaling Chen . Basic Theory and Competitive Exam Analysis of Dynamic Isotope Effect. University Chemistry, 2024, 39(8): 403-410. doi: 10.3866/PKU.DXHX202311093
6. [6]
  Jinfu Ma , Hui Lu , Jiandong Wu , Zhongli Zou . Teaching Design of Electrochemical Principles Course Based on “Cognitive Laws”: Kinetics of Electron Transfer Steps. University Chemistry, 2024, 39(3): 174-177. doi: 10.3866/PKU.DXHX202309052
7. [7]
  Yeyun Zhang , Ling Fan , Yanmei Wang , Zhenfeng Shang . Development and Application of Kinetic Reaction Flasks in Physical Chemistry Experimental Teaching. University Chemistry, 2024, 39(4): 100-106. doi: 10.3866/PKU.DXHX202308044
8. [8]
  Xuzhen Wang , Xinkui Wang , Dongxu Tian , Wei Liu . Enhancing the Comprehensive Quality and Innovation Abilities of Graduate Students through a “Student-Centered, Dual Integration and Dual Drive” Teaching Model: A Case Study in the Course of Chemical Reaction Kinetics. University Chemistry, 2024, 39(6): 160-165. doi: 10.3866/PKU.DXHX202401074
9. [9]
  Dexin Tan , Limin Liang , Baoyi Lv , Huiwen Guan , Haicheng Chen , Yanli Wang . Exploring Reverse Teaching Practices in Physical Chemistry Experiment Courses: A Case Study on Chemical Reaction Kinetics. University Chemistry, 2024, 39(11): 79-86. doi: 10.12461/PKU.DXHX202403048
10. [10]
  Zhuoming Liang , Ming Chen , Zhiwen Zheng , Kai Chen . Multidimensional Studies on Ketone-Enol Tautomerism of 1,3-Diketones By ¹H NMR. University Chemistry, 2024, 39(7): 361-367. doi: 10.3866/PKU.DXHX202311029
11. [11]
  Yiying Yang , Dongju Zhang . Elucidating the Concepts of Thermodynamic Control and Kinetic Control in Chemical Reactions through Theoretical Chemistry Calculations: A Computational Chemistry Experiment on the Diels-Alder Reaction. University Chemistry, 2024, 39(3): 327-335. doi: 10.3866/PKU.DXHX202309074
12. [12]
  Baohua LÜ , Yuzhen LI . Anisotropic photoresponse of two-dimensional layered α-In₂Se₃(2H) ferroelectric materials. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1911-1918. doi: 10.11862/CJIC.20240105
13. [13]
  You Wu , Chang Cheng , Kezhen Qi , Bei Cheng , Jianjun Zhang , Jiaguo Yu , Liuyang Zhang . ZnO/D-A共轭聚合物S型异质结高效光催化产H₂O₂及其电荷转移动力学研究. Acta Physico-Chimica Sinica, 2024, 40(11): 2406027-. doi: 10.3866/PKU.WHXB202406027
14. [14]
  Yan Li , Xinze Wang , Xue Yao , Shouyun Yu . Kinetic Resolution Enabled by Photoexcited Chiral Copper Complex-Mediated Alkene E→Z Isomerization: A Comprehensive Chemistry Experiment for Undergraduate Students. University Chemistry, 2024, 39(5): 1-10. doi: 10.3866/PKU.DXHX202309053
15. [15]
  Tingting Yu , Si Chen , Lianglong Sun , Tongtong Shi , Kai Sun , Xin Wang . Comprehensive Experimental Design for the Photochemical Synthesis, Analysis, and Characterization of Difluoropyrroles. University Chemistry, 2024, 39(11): 196-203. doi: 10.3866/PKU.DXHX202401022
16. [16]
  Yonghui ZHOU , Rujun HUANG , Dongchao YAO , Aiwei ZHANG , Yuhang SUN , Zhujun CHEN , Baisong ZHU , Youxuan ZHENG . Synthesis and photoelectric properties of fluorescence materials with electron donor-acceptor structures based on quinoxaline and pyridinopyrazine, carbazole, and diphenylamine derivatives. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 701-712. doi: 10.11862/CJIC.20230373
17. [17]
  Shengbiao Zheng , Liang Li , Nini Zhang , Ruimin Bao , Ruizhang Hu , Jing Tang . Metal-Organic Framework-Derived Materials Modified Electrode for Electrochemical Sensing of Tert-Butylhydroquinone: A Recommended Comprehensive Chemistry Experiment for Translating Research Results. University Chemistry, 2024, 39(7): 345-353. doi: 10.3866/PKU.DXHX202310096
18. [18]
  Qi Wang , Yicong Gao , Feng Lu , Quli Fan . Preparation and Performance Characterization of the Second Near-Infrared Phototheranostic Probe: A New Design and Teaching Practice of Polymer Chemistry Comprehensive Experiment. University Chemistry, 2024, 39(11): 342-349. doi: 10.12461/PKU.DXHX202404141
19. [19]
  Kai Yang , Gehua Bi , Yong Zhang , Delin Jin , Ziwei Xu , Qian Wang , Lingbao Xing . Comprehensive Polymer Chemistry Experiment Design: Preparation and Characterization of Rigid Polyurethane Foam Materials. University Chemistry, 2024, 39(4): 206-212. doi: 10.3866/PKU.DXHX202308045
20. [20]
  Xiaoling LUO , Pintian ZOU , Xiaoyan WANG , Zheng LIU , Xiangfei KONG , Qun TANG , Sheng WANG . Synthesis, crystal structures, and properties of lanthanide metal-organic frameworks based on 2, 5-dibromoterephthalic acid ligand. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1143-1150. doi: 10.11862/CJIC.20230271

Get Citation

PDF

XML

Metrics

PDF Downloads(501)
Abstract views(584)
HTML views(54)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142